{"id":3488,"date":"2018-09-13T06:00:30","date_gmt":"2018-09-12T21:00:30","guid":{"rendered":"https:\/\/www.muscle-hypertrophy.com\/?p=3488"},"modified":"2019-04-25T08:25:25","modified_gmt":"2019-04-24T23:25:25","slug":"%e7%ad%8b%e5%8f%8e%e7%b8%ae%e3%81%ab%e3%81%8a%e3%81%91%e3%82%8b%e4%b8%bb%e8%a6%81%e3%81%aa%e9%a7%86%e5%8b%95%e5%8a%9b%e3%81%a8%e3%81%97%e3%81%a6%e3%81%ae%e3%82%bf%e3%82%a4%e3%83%81%e3%83%b3%e8%9b%8b","status":"publish","type":"post","link":"https:\/\/www.muscle-hypertrophy.com\/?p=3488","title":{"rendered":"\u7b4b\u53ce\u7e2e\u306b\u304a\u3051\u308b\u4e3b\u8981\u306a\u99c6\u52d5\u529b\u3068\u3057\u3066\u306e\u30bf\u30a4\u30c1\u30f3\u86cb\u767d\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u50cd\u304d"},"content":{"rendered":"

\u3000\u30bf\u30a4\u30c1\u30f3\u306e PEVK \u30c9\u30e1\u30a4\u30f3\u304c\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306b\u5dfb\u304d\u3064\u3044\u3066\u5f3e\u6027\u529b\u3092\u767a\u63ee\u3059\u308b\u3068\u3044\u3046 ‘winding filament’ \u4eee\u8aac\uff08\u30bf\u30a4\u30c1\u30f3\u306f’winding filament’\u306a\u306e\u304b\uff1f\u7b4b\u53ce\u7e2e\u306b\u304a\u3051\u308b\u65b0\u305f\u306a\u306d\u3058\u308c<\/a>\uff09\u306b\u306f\u4e00\u5b9a\u306e\u8aac\u5f97\u529b\u304c\u3042\u3063\u305f\u3082\u306e\u306e\uff0c\u6700\u65b0\u306e\u89b3\u6e2c\u6280\u8853\u306f\u305d\u306e\u4eee\u8aac\u3092\u5426\u5b9a\u3057\u3064\u3064\u3042\u308b\uff0e<\/p>\n

\u3000\u6b66\u5668\u3068\u306a\u3063\u305f\u306e\u306f\u539f\u5b50\u9593\u529b\u9855\u5fae\u93e1<\/a>\u3068\u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u3068\u3044\u3046\u30ca\u30ce\u30e1\u30fc\u30c8\u30eb\u30b5\u30a4\u30ba\u306e\u89b3\u6e2c\u6280\u8853\u304a\u3088\u3073\u64cd\u4f5c\u6280\u8853\u304c\u767a\u5c55\u3057\u305f\u3053\u3068\u3067\u3042\u308b\uff0e\u79d1\u5b66\u6280\u8853\u306e\u767a\u5c55\u304c\u6b21\u306e\u79d1\u5b66\u6280\u8853\u306e\u767a\u5c55\u3092\u4fc3\u3059\u3068\u3044\u3046\u6307\u6570\u95a2\u6570\u7684\u306a\u6280\u8853\u767a\u5c55\u306e\u6642\u4ee3\u306b\u6211\u3005\u306f\u3044\u308b. “Technology will always win.” \u3068\u3044\u3046\u540d\u8a00\u3092\u565b\u307f\u7de0\u3081\u3066\u3044\u308b\uff0e<\/p>\n

\"\"<\/a>
https:\/\/warwick.ac.uk\/fac\/sci\/physics\/current\/postgraduate\/regs\/mpagswarwick\/ex5\/techniques\/structural\/afm\/
\u539f\u5b50\u9593\u529b\u9855\u5fae\u93e1\u306e\u63a2\u91dd<\/figcaption><\/figure>\n

\u3000\u73fe\u5728\u3067\u306f\u30bf\u30a4\u30c1\u30f3\u5206\u5b50\u306e\u6298\u308a\u305f\u305f\u307f\uff08\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\uff09\u3068\u4f38\u5c55\uff08\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\uff09\u304c\u5f3e\u6027\u30a8\u30cd\u30eb\u30ae\u30fc\u306e\u6839\u6e90\u3067\u306f\u306a\u3044\u304b\u3068\u8003\u3048\u3089\u308c\u3066\u304a\u308a, titin folding \u4eee\u8aac<\/strong>\u3068\u3044\u3046\uff0e\u4eca\u56de\u306f\u305d\u306e\u6839\u62e0\u3068\u306a\u308b\u8ad6\u6587\u3092\u7d39\u4ecb\u3059\u308b\uff0e<\/p>\n

\u3000\u6b63\u76f4\u306a\u3068\u3053\u308d\uff0c\u4ffa\u306f\u5316\u5b66\u3084\u7269\u7406\u5b66\u306e\u5c02\u9580\u5bb6\u3067\u306f\u306a\u3044\uff0e\u82f1\u8a9e\u8ad6\u6587\u3092\u548c\u8a33\u3059\u308b\u306b\u3042\u305f\u308a\uff0c\u5c02\u9580\u7528\u8a9e\u306e\u30aa\u30f3\u30d1\u30ec\u30fc\u30c9\u306b\u840e\u7e2e\u3057\u304c\u3061\u3067\u3042\u308b\uff0e\u3053\u306e\u5206\u91ce\u306e\u82f1\u548c\u8f9e\u5178\u304c\u6b32\u3057\u3044\u3068\u5207\u5b9f\u306b\u9858\u3063\u3066\u3044\u308b\uff0e<\/p>\n

<\/p>\n

The work of titin protein folding as a major driver in muscle contraction<\/a> Annu Rev Physiol. 2018 February 10; 80: 327\u2013351. doi:10.1146\/annurev-physiol-021317-121254.<\/h3>\n

Abstract<\/h3>\n

Single molecule atomic force microscopy and magnetic tweezers experiments have demonstrated that titin Ig domains are capable of folding against a pulling force, generating mechanical work which exceeds that produced by a myosin motor. We hypothesize that upon muscle activation, formation of actomyosin crossbridges reduces the force on titin causing entropic recoil of the titin polymer and triggering the folding of the titin Ig domains. In the physiological force range of 4\u201315 pN under which titin operates in muscle, the folding contraction of a single Ig domain can generate 200% of the work of entropic recoil, and occurs at forces which exceed the maximum stalling force of single myosin motors. Thus titin operates like a mechanical battery storing elastic energy efficiently by unfolding Ig domains, and delivering the charge back by folding when the motors are activated during a contraction. We advance the hypothesis that titin folding and myosin activation act as inextricable partners during muscle contraction. <\/p>\n

Keywords<\/h3>\n

Muscle contraction; titin; protein folding; polymer physics; single molecule; force spectroscopy<\/p>\n

\u8981\u7d04<\/h3>\n

\u3000\u5358\u5206\u5b50\u539f\u5b50\u9593\u529b\u9855\u5fae\u93e1\u304a\u3088\u3073\u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u5b9f\u9a13\u304c\u660e\u3089\u304b\u306b\u3057\u305f\u306e\u306f\uff0c\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u304c\u727d\u5f15\u529b\u306b\u5bfe\u6297\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3068\u3044\u3046\u3053\u3068\u3067\u3042\u308a\uff0c\u305d\u308c\u306f\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306b\u3088\u308a\u7523\u751f\u3055\u308c\u308b\u4ed5\u4e8b\u3092\u8d85\u3048\u308b\u6a5f\u68b0\u7684\u4ed5\u4e8b\u3092\u767a\u751f\u3055\u305b\u308b\uff0e\u6211\u3005\u306f\u7b4b\u6d3b\u52d5\u3092\u4eee\u5b9a\u3057\u305f\uff0c\u30a2\u30af\u30c1\u30f3-\u30df\u30aa\u30b7\u30f3\u67b6\u6a4b\u5f62\u6210\u304c\u30bf\u30a4\u30c1\u30f3\u30dd\u30ea\u30de\u30fc\u306e\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u53cd\u52d5\u3092\u5f15\u304d\u8d77\u3053\u3057\uff0c\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u306e\u6298\u308a\u305f\u305f\u307f\u3092\u60f9\u8d77\u3059\u308b\u3053\u3068\u3067\u30bf\u30a4\u30c1\u30f3\u306b\u304b\u304b\u308b\u529b\u3092\u6e1b\u5c11\u3055\u305b\u308b\u3068\uff0e\u7b4b\u8089\u5185\u3067\u30bf\u30a4\u30c1\u30f3\u304c\u52d5\u4f5c\u3059\u308b\u72b6\u6cc1\u4e0b\u3067\u306e 4-15 pN \u3068\u3044\u3046\u751f\u7406\u5b66\u7684\u306a\u529b\u306e\u7bc4\u56f2\u3067\u306f\uff0c\u5358\u4e00\u306e Ig \u30c9\u30e1\u30a4\u30f3\u306e\u53ce\u7e2e\u306e\u6298\u308a\u305f\u305f\u307f\u306f\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u53cd\u52d5\u306e 200 % \u306e\u4ed5\u4e8b\u3092\u767a\u751f\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\uff0c\u5358\u4e00\u306e\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u3092\u5931\u901f\u3055\u305b\u308b\u6700\u5927\u306e\u529b\u3092\u8d85\u3048\u308b\u529b\u3092\u767a\u751f\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u308b\uff0e\u3086\u3048\u306b\u30bf\u30a4\u30c1\u30f3\u306f\u6a5f\u68b0\u5f0f\u30d0\u30c3\u30c6\u30ea\u30fc\u306e\u3088\u3046\u306b\u52d5\u4f5c\u3057, Ig \u30c9\u30e1\u30a4\u30f3\u3092\u4f38\u5c55\u3059\u308b\u3053\u3068\u3067\u5f3e\u6027\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u52b9\u7387\u7684\u306b\u84c4\u7a4d\u3057\uff0c\u53ce\u7e2e\u671f\u306b\u30e2\u30fc\u30bf\u30fc\u304c\u6d3b\u6027\u5316\u3057\u305f\u969b\u306b\u6298\u308a\u305f\u305f\u307f\u306b\u3088\u308a\u96fb\u8377\u3092\u518d\u5145\u96fb\u3059\u308b\uff0e\u6211\u3005\u306f\u3053\u306e\u4eee\u8aac\u3092\u767a\u5c55\u3055\u305b\u305f\uff0e\u3064\u307e\u308a, titin folding \u304a\u3088\u3073\u30df\u30aa\u30b7\u30f3\u6d3b\u6027\u5316\u306f\u7b4b\u53ce\u7e2e\u306b\u304a\u3044\u3066\u4e0d\u53ef\u5206\u306e\u30d1\u30fc\u30c8\u30ca\u30fc\u3068\u3057\u3066\u3075\u308b\u307e\u3046\u306e\u3067\u3042\u308b\u3068\uff0e<\/p>\n

Introduction<\/h3>\n

\u3000\u524d\u4e16\u7d00\u306e\u307b\u3068\u3093\u3069\u306e\u671f\u9593\uff0c\u4eba\u4f53\u306b\u304a\u3051\u308b\u6700\u5927\u306e\u86cb\u767d\u8cea\u3067\u3042\u308b\u30bf\u30a4\u30c1\u30f3\u306e\u5b58\u5728\u306f\u77e5\u3089\u308c\u3066\u3044\u306a\u304b\u3063\u305f (1<\/a>). \u9a5a\u304f\u3053\u3068\u3067\u306f\u306a\u3044\u304c\uff0c\u7b4b\u53ce\u7e2e\u306e\u7406\u8ad6\u306f\u5f53\u6642\u77e5\u3089\u308c\u3066\u3044\u305f\u5206\u5b50\u3067\u3042\u308b\u30a2\u30af\u30c1\u30f3\u3068\u30df\u30aa\u30b7\u30f3\u306b\u57fa\u3065\u3044\u3066\u3044\u305f\uff0e\u3057\u304b\u3057\uff0c\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u6ed1\u8d70\u8aac\u306e\u767a\u898b\u306e\u524d\u591c\u306b\u306f\uff0c\u3044\u304f\u3064\u304b\u7af6\u5408\u3059\u308b\u4eee\u8aac\u304c\u5b58\u5728\u3057\u3066\u304a\u308a\uff0c\u4e0d\u601d\u8b70\u306a\u3053\u3068\u306b\uff0c\u7b4b\u53ce\u7e2e\u306b\u304a\u3051\u308b\u30bf\u30a4\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306e\u3088\u3046\u306a\u5f79\u5272\u3092\u4e88\u8a00\u3057\u305f\u3082\u306e\u3082\u3042\u3063\u305f\uff0e1952 \u5e74\uff0c\u7269\u7406\u5316\u5b66\u8005\u306e Henry Erying \u3068\u89aa\u3057\u304b\u3063\u305f\u7814\u7a76\u8005\u306e Milton Polissar \u306f\u904b\u52d5\u901f\u5ea6\u7406\u8ad6\u3092\u7528\u3044\u3066 100 \u500b\u306e\u72ec\u7acb\u3057\u305f\u53ce\u7e2e\u5358\u4f4d\u304b\u3089\u306a\u308b\u5206\u7bc0\u72b6\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306e\u5b58\u5728\u3092\u793a\u5506\u3057\u3066\u304a\u308a\uff0c\u305d\u306e\u53ce\u7e2e\u5358\u4f4d\u306f\u529b\u306e\u5b58\u5728\u4e0b\u306b\u6a5f\u68b0\u7684\u4ed5\u4e8b\u3092\u306a\u3057\u3066\u77ed\u7e2e\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3068\u3044\u3046\u3082\u306e\u3067\u3042\u3063\u305f (2<\/a>,3<\/a>,4<\/a>,5<\/a>). \u5f7c\u306e\u30a2\u30a4\u30c7\u30a2\u306f\uff0c\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u6ed1\u8d70\u8aac\u304c\u5341\u5206\u306a\u5b9f\u9a13\u7d50\u679c (6<\/a>,7<\/a>,8<\/a>,9<\/a>) \u3092\u4f34\u3063\u3066\u767a\u5c55\u3057\u3066\u304d\u305f\u6642\u306b\u306f\u9053\u306e\u7aef\u306b\u843d\u3061\u3066\u3057\u307e\u3063\u305f\uff0e\u9b45\u60d1\u7684\u306a\u904b\u547d\u306e\u306d\u3058\u308c\u306b\u3088\u308a\uff0c\u6211\u3005\u306f\u3053\u3093\u306b\u3061\uff0c\u4e21\u65b9\u306e\u4eee\u8aac\u304c\u6b63\u3057\u304f\uff0c\u4e21\u65b9\u306e\u6a5f\u5e8f\u304c\u7b4b\u53ce\u7e2e\u306b\u5bc4\u4e0e\u3059\u308b\u3053\u3068\u3092\u77e5\u3063\u3066\u3044\u308b\uff0e\u4eca\u3084\uff0c\u6b21\u306e\u70b9\u3092\u78ba\u7acb\u3059\u308b\u6c7a\u5b9a\u7684\u306a\u6839\u62e0\u304c\u5b58\u5728\u3059\u308b\uff0e\u3064\u307e\u308a\uff0c\u7b4b\u30b5\u30eb\u30b3\u30e1\u30a2\u306f\u4e09\u3064\u306e\u4e3b\u8981\u306a\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\uff0c\u3064\u307e\u308a\u30bf\u30a4\u30c1\u30f3\uff0c\u30a2\u30af\u30c1\u30f3\u304a\u3088\u3073\u30df\u30aa\u30b7\u30f3\u304b\u3089\u306a\u308a\uff0c\u5171\u306b\u5f3e\u6027\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u84c4\u7a4d\u3057\uff0c\u6a5f\u68b0\u7684\u4ed5\u4e8b\u3092\u5b9f\u884c\u3059\u308b\u305f\u3081\u306b\u50cd\u304f (10<\/a>). \u7b4b\u8089\u306b\u304a\u3051\u308b\u4e09\u756a\u76ee\u306e\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u3068\u3057\u3066\u306e\u30bf\u30a4\u30c1\u30f3\u306e\u6839\u62e0\u306f\uff0c\u6700\u521d\u306f\u7591\u3044\u306e\u76ee\u3067\u63a1\u7528\u3055\u308c (11<\/a>), \u30b5\u30eb\u30b3\u30e1\u30a2\u5185\u90e8\u306e\u30bf\u30a4\u30c1\u30f3\u306e\u5b58\u5728\u304c\u5e83\u304f\u53d7\u3051\u5165\u308c\u3089\u308c\u308b\u307e\u3067\u306b\u306f\u6570\u5341\u5e74\u3092\u8981\u3057\u305f (12<\/a>,13<\/a>). \u30df\u30aa\u30b7\u30f3\u306e\u767a\u898b\u3068\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u6ed1\u8d70\u8aac (14<\/a>) \u306e\u767a\u5c55\u306e\u9593\u3067\u4e00\u4e16\u7d00\u8fd1\u304f\u304c\u7d4c\u904e\u3057\uff0c\u6211\u3005\u306f\u9593\u9055\u3044\u306a\u304f\u30bf\u30a4\u30c1\u30f3\u306e\u751f\u7406\u5b66\u7684\u6a5f\u80fd\u306e\u7406\u89e3\u306e\u521d\u671f\u6bb5\u968e\u306b\u9054\u3057\u305f\u3070\u304b\u308a\u3067\u3042\u308b\uff0e<\/p>\n

\u3000\u30bf\u30a4\u30c1\u30f3\u306f\u4eba\u4f53\u306b\u304a\u3044\u3066\u6700\u5927\u7d1a\u306e\u86cb\u767d\u8cea\u3067\u3042\u308a, 370 \u4e07\u30c0\u30eb\u30c8\u30f3 (15<\/a>) \u3068\u65e2\u77e5\u306e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u3067\u6700\u5927\u306e\u5206\u5b50\u91cf\u3092\u6301\u3064\u5358\u4e00\u306e\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u9396\u304b\u3089\u306a\u308b\uff0e\u5404\u30bf\u30a4\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306f\u534a\u30b5\u30eb\u30b3\u30e1\u30a2\u5168\u4f53\u306b\u308f\u305f\u308a\uff0c\u305d\u308c\u3089\u306f 4 \u3064\u306e\u7570\u306a\u308b\u9818\u57df\u306b\u5206\u5272\u3055\u308c\uff0c\u9855\u5fae\u93e1\u3067\u5bb9\u6613\u306b\u8a8d\u8b58\u3067\u304d\u308b (Figure 1A<\/strong>). \u7b4b\u8089\u306e I-band \u306f\u9577\u3055\u304c\u53ef\u5909\u3067\u4e3b\u306b\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u304b\u3089\u306a\u308a\uff0c\u30bf\u30a4\u30c1\u30f3\u3068\u5e73\u884c\u306b\u8d70\u884c\u3057\u3066\u3044\u308b\uff0e\u30b5\u30eb\u30b3\u30e1\u30a2\u306e\u7dca\u5f35\u306b\u4f9d\u5b58\u3057\u3066\uff0c\u30a2\u30af\u30c1\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306e\u3042\u308b\u90e8\u4f4d\u304c A-band \u5185\u90e8\u306b\u5411\u304b\u3063\u3066\u8d70\u884c\u3059\u308b\uff0e\u305d\u306e A-band \u306f\u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u672b\u7aef\u306b\u3088\u308a\u533a\u753b\u3055\u308c\u3066\u304a\u308a\uff0c\u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u3068\u306f 294 \u500b\u306e\u30df\u30aa\u30b7\u30f3\u5206\u5b50\u306e\u675f\u306e\u3053\u3068\u3067\u3042\u308b\u304c\uff0c\u591a\u304f\u306e\u810a\u690e\u52d5\u7269\u306b\u304a\u3044\u3066 1.65 µm \u306e\u9577\u3055\u3067\u898b\u51fa\u3055\u308c\u308b (16<\/a>). \u7b4b\u8089\u306e\u6a2a\u65ad\u9762\u3092\u96fb\u5b50\u9855\u5fae\u93e1\u3067\u89b3\u5bdf\u3059\u308b\u3068\uff0c\u7dda\u7dad\u304c\u516d\u89d2\u683c\u5b50\u72b6\u306b\u914d\u5217\u3057\u3066\u304a\u308a, 6 \u672c\u306e\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u304c\u5404\u30df\u30aa\u30b7\u30f3\u306e\u675f\u306e\u5468\u56f2\u3092\u53d6\u308a\u56f2\u3093\u3067\u3044\u308b\u306e\u304c\u89b3\u5bdf\u3067\u304d\u308b (17<\/a>). \u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306f M-line \u5185\u306e\u86cb\u767d\u8cea\u3067\u4e92\u3044\u306b\u56fa\u5b9a\u3055\u308c\u3066\u304a\u308a\uff0c\u4e00\u65b9\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306f Z \u76e4\u5185\u306e\u30a2\u30eb\u30d5\u30a1\u30a2\u30af\u30c1\u30cb\u30f3\u306b\u3088\u308a\u4fc2\u7559\u3055\u308c\u3066\u3044\u308b\uff0e<\/p>\n

\"\"<\/a>
Figure 1. \u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u539f\u5b50\u9593\u529b\u5206\u5149\u6cd5\u306f\u727d\u5f15\u529b\u306b\u6297\u3059\u308b\u30bf\u30a4\u30c1\u30f3\u518d\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3092\u793a\u3059. (A)<\/strong> \u7d04 2.6 \u03bcm \u306e\u30b5\u30eb\u30b3\u30e1\u30a2\u9577\u3067\u306e\u53b3\u683c\u306a\u9aa8\u683c\u7b4b\u7dda\u7dad\u3092\u63cf\u5199\u3059\u308b\u6a21\u5f0f\u56f3\uff0e\u3053\u306e\u4f38\u5c55\u306b\u304a\u3044\u3066\uff0c\u30bf\u30a4\u30c1\u30f3\u306f 4 pN \u306e\u4eee\u8aac\u7684\u306a\u529b\u306e\u4e0b\u306b\u3042\u308a\uff0c\u3059\u3079\u3066\u306e Ig \u30c9\u30e1\u30a4\u30f3\u306f\u6298\u308a\u7573\u307e\u308c\u3066\u3044\u308b (87<\/a>). \u3042\u308b\u30b5\u30eb\u30b3\u30e1\u30a2\u306e\u534a\u5206\u306b\u30ba\u30fc\u30e0\u30a4\u30f3\u3057\u3066\u307f\u308b: \u30bf\u30a4\u30c1\u30f3\u306f\u5358\u4e00\u306e\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u3067\u3042\u308a, Z \u76e4\uff08N \u672b\u7aef\uff09\u304b\u3089 M \u30d0\u30f3\u30c9\uff08C \u672b\u7aef\uff09\u306b\u5e83\u304c\u308a\uff0c\u6298\u308a\u305f\u305f\u307e\u308c\u305f\u30c9\u30e1\u30a4\u30f3\uff08\u5857\u308a\u3064\u3076\u3057\u305f\u5186\uff09\u304a\u3088\u3073\u975e\u69cb\u9020\u9818\u57df\uff08\u7070\u8272\u306e\u7dda\uff09\u306e\u914d\u5217\u3068\u3057\u3066\u914d\u7f6e\u3055\u308c\u308b. \u30bf\u30a4\u30c1\u30f3\u306f 4 \u3064\u306e\u9818\u57df\u304b\u3089\u306a\u308b: N \u672b\u7aef\u30bb\u30b0\u30e1\u30f3\u30c8\u306f\uff08\u7dd1\u8272\u306e\u5186\uff09\uff0c\u514d\u75ab\u30b0\u30ed\u30d6\u30ea\u30f3 (Ig) \u69d8\u30c9\u30e1\u30a4\u30f3\u304b\u3089\u306a\u308a\uff0c\u30bf\u30a4\u30c1\u30f3\u3092 Z \u76e4\u306b\u4fc2\u7559\u3057\u3066\u3044\u308b. I-band \u30bb\u30b0\u30e1\u30f3\u30c8\u306f\u5f3e\u6027\u8981\u7d20\u3067\u3042\u308a\u4e8c\u7a2e\u985e\u306e\u30c9\u30e1\u30a4\u30f3\u3092\u6709\u3059\u308b\uff0e\u4e00\u5bfe\u306e Ig \u30c9\u30e1\u30a4\u30f3\uff08\u8d64\u3044\u5186\uff09\u3068\u672c\u8cea\u7684\u306b\u7121\u79e9\u5e8f\u306a\u69cb\u9020 (N2-B \u304a\u3088\u3073 PEVK) \u3067\u3042\u308b\uff0e A-band \u9818\u57df\u306f\uff08\u767d\u3044\u5186\uff09\u306f Ig \u304a\u3088\u3073 III \u578b\u30d5\u30a3\u30d6\u30ed\u30cd\u30af\u30c1\u30f3\u30c9\u30e1\u30a4\u30f3\u304b\u3089\u69cb\u6210\u3055\u308c\u3066\u304a\u308a\uff0c\u30bf\u30a4\u30c1\u30f3\u3092\u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306b\u5805\u56fa\u306b\u7d50\u5408\u3059\u308b\u8cac\u4efb\u3092\u6709\u3059\u308b\uff08\u5099\u8003\uff0e\u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306f I-band \u3092\u62e1\u5927\u3059\u308b\u305f\u3081\u5207\u308a\u8a70\u3081\u3089\u308c\u3066\u3044\u308b\uff09\uff0e\u6700\u5f8c\u306b, C \u672b\u7aef\u30bb\u30b0\u30e1\u30f3\u30c8\uff08\u9752\u3044\u5186\uff09\u306f 10 \u500b\u306e Ig \u30c9\u30e1\u30a4\u30f3\u3092\u6709\u3057\u3066\u304a\u308a\uff0c\u30bf\u30a4\u30c1\u30f3\u3092 M-band \u306e\u86cb\u767d\u8cea\u306b\u4fc2\u7559\u3057\u3066\u3044\u308b\uff0e\u4f1d\u7d71\u7684\u306b\uff0c\u30bf\u30a4\u30c1\u30f3\u306f\u53d7\u52d5\u7684\u5f35\u529b\u3092\u8abf\u7bc0\u3059\u308b\u306e\u307f\u3067\uff0c\u3059\u3079\u3066\u306e\u6a5f\u68b0\u7684\u4ed5\u4e8b\u306f\u30a2\u30af\u30c1\u30f3\u30df\u30aa\u30b7\u30f3\u306e\u67b6\u6a4b\u5f62\u6210\u306b\u3088\u308a\u306a\u3055\u308c\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u304d\u305f\uff0e\u7570\u306a\u308b\u30bf\u30a4\u30c1\u30f3\u5207\u7247\u306e\u76f8\u5bfe\u7684\u306a\u9577\u3055\u306f\u4e00\u5b9a\u306e\u7e2e\u5c3a\u3067\u63cf\u304b\u308c\u3066\u3044\u306a\u3044\u3053\u3068\u306b\u7559\u610f\u3055\u308c\u305f\u3044. (B)<\/strong> \u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u5b9f\u9a13\u306e\u5178\u578b\u7684\u306a\u8a18\u9332\uff0e\u6700\u521d\u306b\u9ad8\u3044\u529b\u306e\u6ce2 (105 pN) \u306f 8 \u500b\u306e Ig \u30c9\u30e1\u30a4\u30f3\u3092\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3055\u305b\uff0c\u4e0a\u5411\u304d\u306e\u30b9\u30c6\u30c3\u30d7\uff08\u9ed2\u306e\u77e2\u5370\uff09\u3068\u3057\u3066\u8a18\u9332\u3055\u308c\uff0c\u305d\u306e\u9577\u3055\u306f 25 nm \u3067\u3042\u308b (Figure 2A<\/strong> \u53c2\u7167). \u529b\u3092\u6e1b\u5c11\u3055\u305b\u308b\u3068 (5 pN), \u305d\u306e\u5206\u5b50\u306f\u307e\u305a\u81ea\u767a\u7684\u306b\u5f3e\u6027\u53ce\u7e2e\u3057 (1), \u5f15\u304d\u7d9a\u3044\u3066\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ce\u7e2e\u304c\u8d77\u304d\u308b (2). \u305d\u3053\u3067\u306f\u500b\u3005\u306e\u30c9\u30e1\u30a4\u30f3\u306f\u96e2\u6563\u7684\u306a\u4e0b\u65b9\u30b9\u30c6\u30c3\u30d7\u3068\u3057\u3066\u518d\u6298\u308a\u305f\u305f\u307f\u3055\u308c\uff08\u8d64\u77e2\u5370\uff09\uff0c\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u306f\u77ed\u7e2e\u3059\u308b\uff0e\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u30d1\u30eb\u30b9\u306f\u975e\u751f\u7406\u7684\u306a\u9ad8\u3044\u529b\u3067\u5b9f\u65bd\u3055\u308c, 8 \u500b\u306e\u30c9\u30e1\u30a4\u30f3\u306f\u6570\u79d2\u9593\u3067\u4f38\u5c55\u3055\u308c\u305f\uff0e\u7070\u8272\u306e\u7834\u7dda\u306e\u8ecc\u8de1\u306f 10 pN \u306e\u751f\u7406\u7684\u306a\u529b\u3067\u306e\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u8ecc\u9053\u3092\u793a\u3057\uff0c\u5b8c\u5168\u306a\u4f38\u5c55\u306b 6 \u6642\u9593\u3092\u8981\u3057\u305f\uff0e\u305d\u306e\u529b\u304c 5 pN \u306b\u6e1b\u5c11\u3059\u308b\u3068\uff0c\u5f3e\u6027\u53ce\u7e2e\u306e\u5f37\u3055\u306f\u66f4\u306b\u5c0f\u3055\u304f\u306a\u3063\u305f\u304c\uff0c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ce\u7e2e\u306f\u540c\u3058\u307e\u307e\u3060\u3063\u305f\uff0e (Inset<\/strong>) \u5358\u5206\u5b50\u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u5b9f\u9a13\u3092\u793a\u3059\u6a21\u5f0f\u56f3\uff0e\u30bf\u30a4\u30c1\u30f3\u30dd\u30ea\u86cb\u767d\u8cea\u306f HaloTag \u5171\u6709\u7d50\u5408\u306e\u5316\u5b66\u7684\u6027\u8cea\u306b\u3088\u308a\u30ac\u30e9\u30b9\u8868\u9762\u306b\u4fc2\u7559\u3055\u308c\uff0c\u30d3\u30aa\u30c1\u30f3-\u30b9\u30c8\u30ec\u30d7\u30c8\u30a2\u30d3\u30b8\u30f3\u7d50\u5408\u3092\u7528\u3044\u305f\u8d85\u5e38\u78c1\u6027\u30d3\u30fc\u30ba\u306b\u56fa\u7740\u3055\u308c\u305f\uff0e\u305d\u306e\u529b\u306f\uff0c\u78c1\u6c17\u30d3\u30fc\u30ba\u3092\u542b\u3080\u6d41\u4f53\u30c1\u30e3\u30f3\u30d0\u30fc\u306e\u4e0a\u306b\u4e00\u5bfe\u306e\u6c38\u4e45\u78c1\u77f3\u3092\u914d\u7f6e\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\u5236\u5fa1\u3055\u308c\u308b\uff0e<\/figcaption><\/figure>\n

\u3000\u30bf\u30a4\u30c1\u30f3\u306f\u7b4b\u8089\u306e\u3053\u308c\u3089 4 \u3064\u306e\u9818\u57df\u3067\u5bb9\u6613\u306b\u898b\u3064\u304b\u308b\uff0e\u30bf\u30a4\u30c1\u30f3\u306e N \u672b\u7aef\u306f 9 \u3064\u306e\u514d\u75ab\u30b0\u30ed\u30d6\u30ea\u30f3 (Ig) \u30c9\u30e1\u30a4\u30f3\u304b\u3089\u306a\u308a (Figure 1A<\/strong>, \u7dd1\u8272), Z \u76e4\u306b\u86cb\u767d\u8cea\u3067\u7d50\u5408\u3057\u3066\u3044\u308b\uff0eI-band \u9818\u57df\u306e\u30bf\u30a4\u30c1\u30f3 (Figure 1A<\/strong>, \u8d64) \u306f\u9078\u629e\u7684\u306b\u30b9\u30d7\u30e9\u30a4\u30b9\u3055\u308c\u305f\u30a8\u30af\u30bd\u30f3\u304b\u3089\u306a\u308a, 100 \u500b\u306b\u3082\u53ca\u3076 Ig \u30c9\u30e1\u30a4\u30f3\u3092\u30b3\u30fc\u30c9\u3057\u3066\u304a\u308a\uff0c\u672c\u8cea\u7684\u306b\u7121\u79e9\u5e8f\u306a\u9818\u57df\u3068\u540c\u69d8\u3067\uff0c\u3044\u308f\u3086\u308b N2B \u914d\u5217\u304a\u3088\u3073 PEVK \u914d\u5217\u3068\u547c\u3070\u308c\uff0c\u30d7\u30ed\u30ea\u30f3\uff0c\u30b0\u30eb\u30bf\u30df\u30f3\u9178\uff0c\u30d0\u30ea\u30f3\u304a\u3088\u3073\u30ea\u30b8\u30f3\u6b8b\u57fa\u306b\u5bcc\u3080\uff0e\u7279\u8a18\u3059\u3079\u304d\u91cd\u8981\u306a\u3053\u3068\u3067\u3042\u308b\u304c\uff0c\u9078\u629e\u7684\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u306b\u3088\u308a I-band \u3067\u898b\u3064\u304b\u308b Ig \u30c9\u30e1\u30a4\u30f3\u304a\u3088\u3073\u975e\u69cb\u9020\u7684\u30a2\u30df\u30ce\u9178\u306e\u6570\u306f\uff0c\u5404\u7b4b\u8089\u7fa4\u306b\u3088\u308a\u7570\u306a\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b\uff0e\u6700\u9577\u306e\u9aa8\u683c\u7b4b\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u306f 100 \u500b\u306e Ig \u30c9\u30e1\u30a4\u30f3\u3092\u6709\u3059\u308b\u306e\u306b\uff0c\u5fc3\u7b4b\u7d44\u7e54\u3067\u652f\u914d\u7684\u306a N2B \u30bf\u30a4\u30c1\u30f3\u306e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u306f\u305f\u3063\u305f 47 \u500b\u306e Ig \u30c9\u30e1\u30a4\u30f3\u3068 735 \u500b\u306e\u975e\u69cb\u9020\u30a2\u30df\u30ce\u9178\u6b8b\u57fa\u3092\u6709\u3059\u308b\u306b\u904e\u304e\u306a\u3044\uff0e\u30bf\u30a4\u30c1\u30f3\u306e\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u306e\u5927\u304d\u3055\u306f\uff0c\u3044\u304b\u306a\u308b\u6240\u4e0e\u306e\u30b5\u30eb\u30b3\u30e1\u30a2\u9577\u3067\u30bf\u30a4\u30c1\u30f3\u304c\u529b\u3092\u7d4c\u9a13\u3059\u308b\u304b\u3092\u7406\u89e3\u3059\u308b\u305f\u3081\u306b\u91cd\u8981\u3067\u3042\u308b (18<\/a>). \u30bf\u30a4\u30c1\u30f3\u306e A-band \u9818\u57df\u306f (Figure 1A<\/strong>, \u7070\u8272) Ig \u30c9\u30e1\u30a4\u30f3\u304a\u3088\u3073\u30d5\u30a3\u30d6\u30ed\u30cd\u30af\u30c1\u30f3\u30c9\u30e1\u30a4\u30f3\u304b\u3089\u306a\u308a\uff0c\u305d\u308c\u3089\u306f\u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306b\u56fa\u304f\u7d50\u5408\u3057\u3066\u3044\u308b\uff0e\u6700\u5f8c\u306b\uff0c\u30bf\u30a4\u30c1\u30f3\u306e M-band \u90e8\u4f4d (Figure 1A<\/strong>, \u9752) \u306f\u30bf\u30a4\u30c1\u30f3\u507d\u30ad\u30ca\u30fc\u30bc (19<\/a>,20<\/a>) \u304a\u3088\u3073 10 \u500b\u306e Ig \u30c9\u30e1\u30a4\u30f3\u304b\u3089\u306a\u308a\uff0c\u96a3\u63a5\u3059\u308b\u30bf\u30a4\u30c1\u30f3\u304a\u3088\u3073\u4ed6\u306e\u69cb\u9020\u86cb\u767d\u8cea\u3092\u7d50\u5408\u3057\u3066\u3044\u308b\uff0e\u3053\u306e\u30ec\u30d3\u30e5\u30fc\u306f\u4e3b\u306b\u30bf\u30a4\u30c1\u30f3\u306e\u5f3e\u6027 I-band \u5207\u7247\u306e\u529b\u306e\u767a\u751f\u80fd\u529b\u306b\u95a2\u4fc2\u3057\u3066\u3044\u308b\u304c\uff0c\u30bf\u30a4\u30c1\u30f3\u3068\u7b4b\u8089\u306e\u5fae\u7d30\u69cb\u9020\u3092\u8b70\u8ad6\u3057\u3066\u3044\u308b\u512a\u79c0\u306a\u8cc7\u6e90\u306f\u591a\u6570\u5b58\u5728\u3059\u308b (21<\/a>,22<\/a>,23<\/a>,24<\/a>,25<\/a>). <\/p>\n

\u3000\u7b4b\u53ce\u7e2e\u306e\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u6ed1\u8d70\u8aac\u306b\u304a\u3044\u3066\u306f, ATP \u52a0\u6c34\u5206\u89e3\u3068\u7121\u6a5f\u30ea\u30f3\u9178\u306e\u653e\u51fa\u304c\u30df\u30aa\u30b7\u30f3\u9838\u90e8\u306e\u69cb\u9020\u5909\u5316\u3092\u3082\u305f\u3089\u3057\uff0c\u30df\u30aa\u30b7\u30f3\u982d\u90e8\u304c\u30a2\u30af\u30c1\u30f3\u306b\u7d50\u5408\u3059\u308b\uff0e\u3053\u306e\u52d5\u4f5c\u304c\u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u3092\u52d5\u304b\u3057\uff0c\u30a2\u30af\u30c1\u30f3\u306b\u5bfe\u3059\u308b\u529b\u3092\u767a\u751f\u3055\u305b\u308b (9<\/a>). \u7b4b\u53ce\u7e2e\u306e\u969b\u306b\u30df\u30aa\u30b7\u30f3\u304c\u5358\u72ec\u3067\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u3092\u6ed1\u8d70\u3055\u305b, ATP \u306e\u5316\u5b66\u30a8\u30cd\u30eb\u30ae\u30fc\u304c\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u5468\u671f\u306b\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u4f9b\u7d66\u3059\u308b\u3068\u3044\u3046\u70b9\u306f\u9593\u9055\u3044\u306a\u3044 (26<\/a>,27<\/a>,28<\/a>,29<\/a>) \u304c\uff0c\u77ed\u7e2e\u6642\u306e\u30bf\u30a4\u30c1\u30f3\u306e\u5f79\u5272\u306f\u307b\u3068\u3093\u3069\u7406\u89e3\u3055\u308c\u3066\u304a\u3089\u305a\uff0c\u660e\u78ba\u306b\u3059\u308b\u305f\u3081\u306b\u6301\u7d9a\u7684\u306a\u7126\u70b9\u3092\u5f53\u3066\u308b\u3053\u3068\u304c\u7b4b\u8089\u30b3\u30df\u30e5\u30cb\u30c6\u30a3\u306b\u6c42\u3081\u3089\u308c\u3066\u3044\u305f\uff0e\u30bf\u30a4\u30c1\u30f3\u304c\u3044\u304b\u306b\u3057\u3066\u7b4b\u53ce\u7e2e\u306e\u969b\u306b\u529b\u3092\u767a\u751f\u3059\u308b\u306e\u304b\u306b\u3064\u3044\u3066\u306f\uff0c\u3044\u304f\u3064\u304b\u7af6\u5408\u3059\u308b\u4eee\u8aac\u304c\u5b58\u5728\u3059\u308b (22<\/a>,30<\/a>,31<\/a>,32<\/a>) \u304c\uff0c\u7b4b\u7dda\u7dad\u30ec\u30d9\u30eb\u3067\u5206\u5b50\u6a5f\u69cb\u3092\u89e3\u660e\u3059\u308b\u8a66\u307f\u306f\u5931\u6557\u3057\u3066\u304d\u305f\uff0e\u904e\u53bb 20 \u5e74\u9593\u306b\u308f\u305f\u308a\uff0c\u30bf\u30f3\u30d1\u30af\u8cea\u5de5\u5b66\u3068\u7d44\u307f\u5408\u308f\u305b\u305f\u5206\u5b50\u9593\u529b\u5206\u5149\u6cd5\u306b\u304a\u3051\u308b\u9032\u6b69\u306b\u3088\u308a\uff0c\u529b\u306e\u4e0b\u3067\u306e\u30bf\u30a4\u30c1\u30f3\u306e\u529b\u5b66\u306e\u7814\u7a76\u304c\u9032\u3093\u3067\u304d\u305f (10<\/a>,33<\/a>,34<\/a>,35<\/a>,36<\/a>,37<\/a>,38<\/a>,39<\/a>). \u5206\u5b50\u9593\u529b\u5206\u5149\u6cd5\u306e\u30c7\u30fc\u30bf\u306e\u793a\u3059\u3068\u3053\u308d\u306f\uff0c\u30bf\u30a4\u30c1\u30f3\u306f Ig \u30c9\u30e1\u30a4\u30f3\u5206\u5b50\u306e\u591a\u304f\u306e\u8155\u3092\u4f38\u5c55\u3057\u305f\u308a\u518d\u5ea6\u6298\u308a\u305f\u305f\u3093\u3060\u308a\u3059\u308b\u3053\u3068\u3067\u5f3e\u6027\u30a8\u30cd\u30eb\u30ae\u30fc\u306e\u307b\u3068\u3093\u3069\u3092\u84c4\u7a4d\u3057\uff0c\u63d0\u4f9b\u3059\u308b\u3068\u3044\u3046\u3082\u306e\u3067\u3042\u308b\uff0e\u4eca\u3084\u6211\u3005\u306e\u8cac\u52d9\u306f\uff0c\u30bf\u30a4\u30c1\u30f3\u6298\u308a\u305f\u305f\u307f\u306e\u529b\u5b66\u3092\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u6ed1\u8d70\u8aac\u306e\u67a0\u7d44\u307f\u306b\u7d44\u307f\u8fbc\u3080\u3053\u3068\u3067\uff0c\u7b4b\u53ce\u7e2e\u306e\u77e5\u898b\u3092\u767a\u5c55\u3055\u305b\u308b\u3053\u3068\u3067\u3042\u308b\uff0e\u3055\u3042\uff0c\u3084\u3063\u3066\u307f\u3088\u3046\uff0e<\/p>\n

Studies of titin under force<\/h3>\n

\u3000\u5316\u5b66\u7684\u30fb\u71b1\u7684\u5909\u6027\u306a\u3069\u306e\u4f1d\u7d71\u7684\u306a\u904e\u534a\u6570\u306e\u751f\u5316\u5b66\u6280\u8853\u306f\u7b4b\u8089\u5185\u306e\u30bf\u30a4\u30c1\u30f3\u306e\u6a5f\u80fd\u3092\u7406\u89e3\u3059\u308b\u306b\u306f\u5b9f\u7528\u7684\u3067\u306f\u306a\u3044. \u751f\u4f53\u5185\u3067\u30bf\u30a4\u30c1\u30f3\u306f 15 pN \u307e\u3067\u306e\u6a5f\u68b0\u7684\u8ca0\u8377\u306e\u3082\u3068\u3067\u52d5\u4f5c\u3059\u308b\u304c\uff0c\u305d\u308c\u306f Z \u76e4\u3078\u306e\u4ed8\u7740\u3068\u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u5148\u7aef\u3068\u306e\u9593\u306e\u30d9\u30af\u30c8\u30eb\u306b\u6cbf\u3063\u3066\u9069\u7528\u3055\u308c\u308b\u8ca0\u8377\u3067\u3042\u308b\uff0e\u539f\u5b50\u9593\u529b\u9855\u5fae\u93e1 (AFM) \u3084\u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u304a\u3088\u3073\u5149\u30d4\u30f3\u30bb\u30c3\u30c8\u306a\u3069\u306e\u5358\u5206\u5b50\u9593\u529b\u5206\u5149\u6cd5\u6280\u8853\u306f\uff0c\u751f\u4f53\u5185\u3067\u7d4c\u9a13\u3059\u308b\u306e\u3068\u540c\u3058\u5354\u8abf\u53cd\u5fdc\u3092\u901a\u3058\u3066\u30bf\u30a4\u30c1\u30f3\u306e\u30ca\u30ce\u30b9\u30b1\u30fc\u30eb\u306e\u6a5f\u5e8f\u3092\u6398\u308a\u4e0b\u3052\uff0c\u904e\u534a\u6570\u306e\u6280\u8853\u3088\u308a\u3082\u3063\u3068\u751f\u7406\u5b66\u7684\u306a\u6d1e\u5bdf\u3092\u3082\u305f\u3089\u3057\u3066\u304f\u308c\u308b\uff0e\u86cb\u767d\u8cea\u30ec\u30d9\u30eb\u306e\u30b3\u30f3\u30d5\u30a9\u30e1\u30fc\u30b7\u30e7\u30f3\u5909\u5316\u3092\u5f15\u304d\u8d77\u3053\u3059\u7d30\u80de\u30d7\u30ed\u30bb\u30b9\u306e\u591a\u69d8\u6027\u3092\u4fee\u98fe\u3059\u308b\u529b\u306e\u65b0\u3057\u3044\u89b3\u70b9\u306b\u3088\u308a\uff0c\u3053\u308c\u3089\u306e\u624b\u6cd5\u306f\u6025\u901f\u306b\u7d30\u80de\u306e\u6a5f\u5e8f\u3092\u7406\u89e3\u3059\u308b\u305f\u3081\u306e\u4e0d\u53ef\u6b20\u306e\u6280\u8853\u3068\u306a\u308a\u3064\u3064\u3042\u308b\uff0e<\/p>\n

\u3000\u86cb\u767d\u8cea\u5206\u5b50\u9593\u529b\u5206\u5149\u6cd5\u306e\u5b9f\u9a13\u306e\u5b9f\u65bd\u306b\u306f\uff0c\u56fa\u5b9a\u8868\u9762\u3068\u9855\u5fae\u93e1\u30d7\u30ed\u30fc\u30d6\u306e\u9593\u306e\u5358\u4e00\u306e\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u4e00\u672c\u9396\u3092\u5fc5\u8981\u3068\u3057\uff0c\u305d\u306e\u30d7\u30ed\u30fc\u30d6\u306f\u5f35\u529b\u3092\u304b\u3051\u3089\u308c\u308b\u3088\u3046\u914d\u7f6e\u3067\u304d\u308b\u3082\u306e\u3067\u3042\u308b\uff0eAFM \u7814\u7a76\u306e\u305f\u3081\u306b\uff0c\u5f35\u529b\u30d7\u30ed\u30fc\u30d6\u306f\u7a92\u5316\u30b1\u30a4\u7d20\u88fd\u306e\u30de\u30a4\u30af\u30ed\u30ab\u30f3\u30c1\u30ec\u30d0\u30fc\u3067\u3042\u308a\uff0c\u86cb\u767d\u8cea\u672b\u7aef\u306b\u5171\u6709\u7d50\u5408\u3067\u3082\u975e\u5171\u6709\u7d50\u5408\u3067\u3082\u53d6\u308a\u4ed8\u3051\u308b\u3053\u3068\u304c\u3067\u304d\u308b (40<\/a>,41<\/a>). \u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u306b\u3064\u3044\u3066\u306f\uff0c\u78c1\u754c\u3092\u53d7\u3051\u305f\u78c1\u6027\u4f53\u7c92\u5b50\u3092\u4ed8\u7740\u3055\u305b\u305f\u86cb\u767d\u8cea\u3092\u4e00\u672c\u9396\u306b\u5f15\u304d\u4f38\u3070\u3059 (10<\/a>,40<\/a>,42<\/a>,43<\/a>). \u5149\u30d4\u30f3\u30bb\u30c3\u30c8\u306f\u30ec\u30fc\u30b6\u30fc\u30c8\u30e9\u30c3\u30d7\u3092\u7528\u3044\u308b\u3082\u306e\u3067\uff0c\u8a98\u96fb\u4f53\u7c92\u5b50\u3092\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u306b\u4ed8\u7740\u3055\u305b\u529b\u3092\u9069\u7528\u3059\u308b (44<\/a>,45<\/a>). \u3059\u3079\u3066\u306e\u4f8b\u3067\uff0c\u5f35\u529b\u30d7\u30ed\u30fc\u30d6\u306e\u4f4d\u7f6e\u306f\u9ad8\u901f\u30ec\u30fc\u30b6\u30fc\u691c\u51fa\u5668\u307e\u305f\u306f\u30d3\u30c7\u30aa\u9855\u5fae\u93e1\u3067\u30ca\u30ce\u30e1\u30fc\u30bf\u30fc\uff0c\u307e\u305f\u306f\u6700\u4f4e\u3067\u3082\u30aa\u30f3\u30b0\u30b9\u30c8\u30ed\u30fc\u30e0\u7cbe\u5ea6\u3067\u8a08\u6e2c\u3055\u308c\u308b\uff0e\u4f38\u5c55\u306b\u3088\u308b\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u9577\u306e\u6f38\u9032\u7684\u306a\u5909\u5316\uff0c\u307e\u305f\u306f\u30c9\u30e1\u30a4\u30f3\u306e\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304a\u3088\u3073\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u3088\u308b\u7a81\u7136\u306e\u5909\u5316\u306f\u9ad8\u3044\u6642\u7a7a\u9593\u89e3\u50cf\u5ea6\u3067\u8a08\u6e2c\u53ef\u80fd\u3067\u3042\u308b (46<\/a>,47<\/a>,48<\/a>,49<\/a>). <\/p>\n

\u3000\u521d\u671f\u306e\u5358\u5206\u5b50\u9593\u529b\u5206\u5149\u6cd5\u306e\u5b9f\u9a13\u3067\u306f, DNA (50<\/a>,51<\/a>) \u3068\u591a\u7cd6\u985e (52<\/a>,53<\/a>) \u306e\u6a5f\u5e8f\uff0c\u53d7\u5bb9\u4f53\u3068\u30ea\u30ac\u30f3\u30c9\u306e\u89e3\u96e2 (54<\/a>), \u30e2\u30fc\u30bf\u30fc\u86cb\u767d\u529b\u5b66 (26<\/a>,44<\/a>), \u305d\u3057\u3066\u9175\u7d20\u306e\u30b3\u30f3\u30d5\u30a9\u30e1\u30fc\u30b7\u30e7\u30f3\u5909\u5316 (55<\/a>) \u3092\u8abf\u67fb\u3057\u305f\uff0e\u3057\u304b\u3057\uff0c\u3054\u304f\u521d\u671f\u306e\u30ca\u30ce\u30e1\u30fc\u30c8\u30eb\u89e3\u50cf\u5ea6\u3067\u306e\u53ef\u9006\u7684\u306a\u86cb\u767d\u8cea\u306e\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u8a18\u9332\u306f\uff0c\u30de\u30a4\u30af\u30ed\u30e1\u30fc\u30c8\u30eb\u9577\u306e\u30bf\u30a4\u30c1\u30f3\u3092\u57fa\u8cea\u3068\u3057\u3066\u5b9f\u884c\u3055\u308c\u305f (37<\/a>). AFM \u3092\u7528\u3044\u3066\uff0c\u30a6\u30b7\u306e\u7d44\u7e54\u304b\u3089\u7cbe\u88fd\u3055\u308c\u305f\u5929\u7136\u306e\u30bf\u30a4\u30c1\u30f3\u306f\u9ad8\u3044\u529b\u3067\u4f38\u5c55\u3055\u308c\uff0c\u30ab\u30f3\u30c1\u30ec\u30d0\u30fc\u5148\u7aef\u3092\u5f15\u304d\u4e0a\u3052\u9762\u306b\u623b\u3059\u3053\u3068\u3067\u518d\u30d5\u30a9\u30fc\u30eb\u30c9\u3067\u304d\u308b\u3088\u3046\u306b\u3057\u305f\uff0e\u30bf\u30a4\u30c1\u30f3\u306f\u3053\u308c\u3089\u306e\u5b9f\u9a13\u306e\u305f\u3081\u306e\u81ea\u7136\u306e\u9078\u629e\u3067\u3042\u3063\u305f\uff0e\u3068\u3044\u3046\u306e\u306f\uff0c\u30bf\u30a4\u30c1\u30f3\u306f\u591a\u304f\u306e\u30c9\u30e1\u30a4\u30f3\u304c\u307b\u307c\u540c\u3058\u5927\u304d\u3055\u306e\u514d\u75ab\u30b0\u30ed\u30d6\u30ea\u30f3\u304b\u3089\u306a\u308a\uff0c\u4e00\u5b9a\u901f\u5ea6\u3067\u5358\u5206\u5b50\u3092\u727d\u5f15\u3059\u308b\u5b9f\u9a13\u306b\u304a\u3044\u3066\u7279\u5fb4\u7684\u306a\u300c\u92f8\u6b6f\u72b6\u300d\u30d1\u30bf\u30fc\u30f3\u3092\u5448\u3057\u3066\u3044\u305f\u304b\u3089\u3067\u3042\u308b\uff0e\u540c\u69d8\u306e\u7d50\u679c\u304c\u5149\u30d4\u30f3\u30bb\u30c3\u30c8\u3092\u7528\u3044\u3066\u5929\u7136\u306e\u30bf\u30a4\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u3092\u9ad8\u3044\u529b\u3067\u4f38\u5c55\u3059\u308b\u5b9f\u9a13\u3067\u793a\u3055\u308c\u3066\u304a\u308a\uff0c\u86cb\u767d\u8cea\u306e\u6bb5\u968e\u7684\u69d8\u5f0f\u307e\u305f\u306f\u6f38\u5897\u69d8\u5f0f\u304c\u5f97\u3089\u308c\uff0c\u3053\u308c\u306f\u5358\u4e00\u30c9\u30e1\u30a4\u30f3\u306e\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304c\u8d77\u304d\u305f\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u308b (35<\/a>,39<\/a>). \u907a\u4f1d\u5b50\u7d44\u63db\u3048\u86cb\u767d\u8cea\u5de5\u5b66\u304a\u3088\u3073\u5206\u5b50\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u6280\u8853\u306f\uff0c\u975e\u5e38\u306b\u8a73\u7d30\u306b\u7814\u7a76\u3055\u308c\u305f\u5f3e\u6027\u30bf\u30a4\u30c1\u30f3 I-band \u306e\u4f4d\u7f6e\u3092\u3088\u308a\u5c0f\u3055\u304f\uff0c\u3088\u308a\u5236\u5fa1\u53ef\u80fd\u306b\u3057\u3066\u3044\u308b (33<\/a>,56<\/a>). \u3053\u308c\u306b\u3088\u308a Ig \u30c9\u30e1\u30a4\u30f3\u5185\u90e8\u306e\u6a5f\u68b0\u7684\u968e\u5c64\u304c\u660e\u3089\u304b\u306b\u306a\u308a\uff0c\u8fd1\u4f4d Ig \u30c9\u30e1\u30a4\u30f3\uff08N \u672b\u7aef\u306b\u8fd1\u3044\u90e8\u4f4d\uff09\u306e\u6a5f\u68b0\u7684\u5b89\u5b9a\u6027\u304c I-band \u306e\u9060\u4f4d Ig \u30c9\u30e1\u30a4\u30f3\u3088\u308a\u3082\u4f4e\u3044\u3053\u3068\u304c\u5206\u304b\u3063\u305f\uff0e\u3055\u3089\u306b, HaloTag \u30a2\u30f3\u30ab\u30fc\u306e\u86cb\u767d\u8cea\u306e N \u307e\u305f\u306f C \u672b\u7aef\u3078\u306e\u5171\u6709\u7d50\u5408\u306e\u5de5\u5b66\u306f\uff0c\u5f37\u3044\u529b\u3067\u9577\u6642\u9593\u306e\u7814\u7a76\u306e\u305f\u3081\u306e\u907a\u4f1d\u5b50\u7d44\u307f\u63db\u3048\u30bf\u30a4\u30c1\u30f3\u306e\u7279\u7570\u7684\u56fa\u5b9a\u5316\u3078\u306e\u9053\u3092\u958b\u3044\u305f (41<\/a>,49<\/a>,57<\/a>). <\/p>\n

\u3000\u904e\u53bb 20 \u5e74\u9593\u306b\u308f\u305f\u308a\uff0c\u5206\u5b50\u9593\u529b\u5206\u5149\u6cd5\u306f\u8a08\u88c5\u3068\u6280\u8853\u306b\u304a\u3044\u3066\u5927\u304d\u306a\u6539\u5584\u306e\u6069\u6075\u3092\u53d7\u3051\u305f\uff0e\u96fb\u4f4d\u56fa\u5b9a\u6cd5\u3092\u9451\u307f\u308b\u306b\uff0c\u5358\u4e00\u306e\u30a4\u30aa\u30f3\u30c1\u30e3\u30cd\u30eb\u30b2\u30fc\u30c8\u306e\u5b9f\u9a13\u306e\u521d\u3081\u3066\u306e\u6210\u529f\u3092\u652f\u3048\u305f\u3082\u306e\u3067\u3042\u308b\u304c, AFM \u306e\u305f\u3081\u306e\u539f\u5b50\u9593\u529b\u56fa\u5b9a\u6cd5\u306e\u767a\u5c55\u306f\u86cb\u767d\u8cea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u8ad6\u306b\u3064\u3044\u3066\u6211\u3005\u306e\u7406\u89e3\u3092\u5927\u304d\u304f\u9032\u6b69\u3055\u305b\u305f (34<\/a>,38<\/a>,58<\/a>). \u539f\u5b50\u9593\u529b\u56fa\u5b9a\u5206\u5149\u6cd5\u306f\u660e\u767d\u306b\uff0c\u5b9a\u7fa9\u3055\u308c\u305f\u727d\u5f15\u529b\u306e\u4e0b\u3067\u306e\uff0c\u5358\u4e00\u306e\u86cb\u767d\u8cea\u30da\u30d7\u30c1\u30c9\u306e\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304a\u3088\u3073\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u8ecc\u8de1\u306b\u4ed8\u304d\u6dfb\u3044\uff0c\u30aa\u30f3\u30b0\u30b9\u30c8\u30ed\u30fc\u30e0\u5358\u4f4d\u306e\u89e3\u50cf\u5ea6\u3067\u86cb\u767d\u8cea\u306e\u672b\u7aef\u304b\u3089\u672b\u7aef\u307e\u3067\u306e\u9577\u3055\u3092\u8ffd\u8de1\u3059\u308b\uff0e\u3053\u308c\u306b\u3088\u308a\uff0c\u529b\u5b66\u3068\u5916\u529b\u4f9d\u5b58\u6027\u306e\u86cb\u767d\u8cea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3084\u4ed6\u306e\u751f\u5316\u5b66\u53cd\u5fdc\u3068\u306e\u7814\u7a76\u304c\u9032\u3093\u3060 (59<\/a>,60<\/a>). \u3082\u3063\u3068\u6700\u8fd1\u3067\u306f\uff0c\u53d7\u52d5\u7684\u306b\u7121\u9650\u306e\u5e2f\u57df\u5e45\u3067\u52d5\u4f5c\u3059\u308b\u86cb\u767d\u8cea\u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u306e\u767a\u5c55\u306b\u3088\u308a, 15 pN \u4ee5\u4e0b\u306e\u529b\u3067\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u306b\u304a\u3051\u308b\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u30fb\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u9077\u79fb\u5e73\u8861\u3092\u76f4\u63a5\u89b3\u6e2c\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u3063\u305f (10<\/a>,43<\/a>). \u3053\u306e\u7a2e\u306e\u5b9f\u9a13\u306f\uff0c\u751f\u4f53\u5185\u3067\u52d5\u4f5c\u3059\u308b\u306e\u3068\u6975\u3081\u3066\u3088\u304f\u4f3c\u305f\u72b6\u614b\u3067\u306e\u30bf\u30a4\u30c1\u30f3\u3092\u63a2\u7d22\u3059\u308b\u305f\u3081\u306e\u81ea\u7136\u306a\u30b7\u30ca\u30ea\u30aa\u3092\u63d0\u4f9b\u3057\u3066\u304f\u308c\u308b. Figure 1B<\/strong> \u306b\u5b9f\u9a13\u4f8b\u3092\u56f3\u793a\u3057\u305f\u304c\uff0c\u4eca\u65e5\u6211\u3005\u304c\u30bf\u30a4\u30c1\u30f3\u306e\u6a5f\u5e8f\u306b\u3064\u3044\u3066\u77e5\u3063\u3066\u3044\u308b\u3053\u3068\u306e\u591a\u304f\u3092\u8981\u7d04\u3057\u3066\u3042\u308b\uff0e\u3053\u306e\u5b9f\u9a13\u306b\u304a\u3044\u3066\u306f 8 \u672c\u306e\u8155\u3092\u6301\u3064\u5358\u4e00\u306e\u86cb\u767d\u8cea Ig 91 (I91) \u30c9\u30e1\u30a4\u30f3\u304c\u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u88c5\u7f6e\u306e\u4e2d\u3067\u4e00\u5b9a\u306e\u4f38\u5c55\u529b\u306e\u4e0b\u306b\u56fa\u5b9a\u3055\u308c\u3066\u3044\u308b\uff0e\u91cd\u8981\u306a\u3053\u3068\u306a\u306e\u3067\u66f8\u3044\u3066\u304a\u304f\u304c\uff0c\u3053\u306e I91 \u30c9\u30e1\u30a4\u30f3\u306f\u53e4\u3044\u6587\u732e (36<\/a>,61<\/a>) \u3067\u8a00\u53ca\u3055\u308c\u3066\u3044\u308b I27 \u30c9\u30e1\u30a4\u30f3\u3068\u540c\u4e00\u3067\u3042\u308a\uff0c\u547d\u540d\u6cd5\u304c\u5909\u5316\u3057\u305f\u306e\u307f\u3067\u3042\u308b\uff0e\u305d\u306e\u86cb\u767d\u8cea\u306f\u5f53\u521d, 105 pN \u3082\u306e\u975e\u751f\u7406\u7684\u306a\u5f37\u3044\u529b\u3067\u3042\u3063\u3068\u3044\u3046\u9593\u306b\u3059\u3079\u3066\u306e Ig \u30c9\u30e1\u30a4\u30f3\u304c\u4f38\u5c55\u3057\u3066\u3057\u307e\u3063\u305f\uff0e\u3053\u308c\u306f\u6700\u521d\u306b\u5f3e\u6027\u306e\u4f38\u5c55\u304c\u8d77\u3053\u308a\uff0c\u5f15\u304d\u7d9a\u3044\u3066\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3068 8 \u3064\u306e Ig \u30c9\u30e1\u30a4\u30f3\u306e\u4f38\u9577\u306b\u306f\u305f\u3063\u305f 20 \u79d2\u9593\u3057\u304b\u304b\u304b\u3089\u306a\u304b\u3063\u305f\uff0e\u5bfe\u7167\u7684\u306b, 10 pN \u306e\u751f\u7406\u7684\u306a\u529b\u3067\u306f I91 \u306e\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306f\u6570\u6642\u9593\u3092\u8981\u3057\u305f (Figure 1B<\/strong> \u7070\u8272\u70b9\u7dda). \u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u306b\u304b\u304b\u308b\u529b\u304c 5 pN \u306b\u307e\u3067\u6e1b\u5c11\u3059\u308b\u3068\uff0c\u30bf\u30a4\u30c1\u30f3\u306f\u5373\u6642\u5f3e\u529b\u30b3\u30a4\u30eb\u306b\u5909\u5316\u3057\u3066\u5f15\u304d\u7d9a\u304d\u6bb5\u968e\u7684\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53cd\u5fdc\u3092\u793a\u3057\u305f\uff0e<\/p>\n

\u3000\u539f\u5b50\u9593\u529b\u5206\u5149\u6cd5\u306b\u3088\u308a\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c9\u3057\u305f\u6700\u521d\u306e\u86cb\u767d\u8cea\u304c\u30bf\u30a4\u30c1\u30f3\u3067\u3042\u3063\u305f\u305f\u3081\uff0c\u5f15\u304d\u7d9a\u3044\u3066\u86cb\u767d\u8cea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u7269\u7406\u5b66\u3092\u7406\u89e3\u3059\u308b\u30e2\u30c7\u30eb\u3068\u306a\u3063\u305f\uff0e\u30bf\u30a4\u30c1\u30f3\u306e\u3088\u3046\u306b\u7403\u72b6\u306b\u6298\u308a\u305f\u305f\u307e\u308c\u308b\u3053\u3068\u306e\u6a5f\u68b0\u7684\u5b89\u5b9a\u6027\u306f\u9759\u96fb\u76f8\u4e92\u4f5c\u7528\uff0c\u6c34\u7d20\u7d50\u5408\u304a\u3088\u3073\u758e\u6c34\u76f8\u4e92\u4f5c\u7528\u306b\u4f9d\u5b58\u3057\uff0c\u86cb\u767d\u8cea\u306e\u914d\u5217\u304a\u3088\u3073\u4f4d\u76f8\u306b\u3088\u308a\u652f\u914d\u3055\u308c\u308b. Figure 2A<\/strong> \u306b, 6 pN \u306e\u6a5f\u68b0\u7684\u529b\u3067\u53ef\u9006\u7684\u306b\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3059\u308b\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u306e\u8a73\u7d30\u56f3\u3092\u793a\u3059\uff0e\u529b\u306e\u5927\u90e8\u5206\u306f\u6700\u521d\u306e (A-A’) \u3068\u6700\u5f8c\u306e (G) \u30d9\u30fc\u30bf\u9396\u3068\u306e\u9593\u306e\u6c34\u7d20\u7d50\u5408\u306b\u3088\u308a\u7dad\u6301\u3055\u308c (62<\/a>), \u5076\u767a\u7684\u306b\u30c9\u30e1\u30a4\u30f3\u306e\u5b8c\u5168\u306a\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304c\u8d77\u304d\u308b\uff0e\u3053\u308c\u304c\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u306b\u3068\u3063\u3066\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304c\u8d77\u304d\u308b\u6a5f\u5e8f\u3068\u8003\u3048\u3089\u308c\u3066\u304a\u308a, I91 \u3068\u306e\u9ad8\u3044\u69cb\u9020\u7684\u985e\u4f3c\u6027\u304c\u8a8d\u3081\u3089\u308c\u308b\uff0e\u4e00\u65e6\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304c\u8d77\u304d\u308b\u3068\uff0c\u305d\u306e\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u306f\u30e9\u30f3\u30c0\u30e0\u30b3\u30a4\u30eb\u3068\u306a\u308b\uff0e\u305d\u306e\u610f\u5473\u3059\u308b\u3068\u3053\u308d\u306f\uff0c\u672b\u7aef\u9593\u306e\u8ddd\u96e2\u306f\uff0c\u3088\u304f\u77e5\u3089\u308c\u3066\u3044\u308b\u30dd\u30ea\u30de\u30fc\u7269\u7406\u5b66\u6cd5\u5247\u306b\u3088\u308b\u5f35\u529b\u3068\u898f\u6a21\u306b\u3088\u308a\u5b9a\u7fa9\u3055\u308c\u308b\u3068\u3044\u3046\u3053\u3068\u3067\u3042\u308b (63<\/a>). \u4f8b\u3048\u3070, 6 pN \u306b\u304a\u3044\u3066\uff0c\u6298\u308a\u305f\u305f\u307e\u308c\u305f I91 \u30c9\u30e1\u30a4\u30f3\u306f\u672b\u7aef\u304b\u3089\u672b\u7aef\u3078\u306e\u9577\u3055\u304c\u7d04 4 nm \u3067\u3042\u308a\uff08\u539f\u5b50\u5ea7\u6a19\u304b\u3089\u63a8\u6e2c\uff0e\u8a73\u7d30\u306f PDB 1WAA \u53c2\u7167\u306e\u3053\u3068(64<\/a>)\uff09\uff0c\u4e00\u65b9\u3067\u4f38\u5c55\u3055\u308c\u305f\u30dd\u30ea\u30de\u30fc\u306f 17 nm \u306e\u9577\u3055\u3092\u6301\u3064\uff0e\u3086\u3048\u306b\uff0c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3068\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u9077\u79fb\u671f\u306b\u89b3\u5bdf\u3055\u308c\u305f\u30b9\u30c6\u30c3\u30d7\u306f 6 pN \u306e\u529b\u306e\u4e0b\u3067\u306f 13 nm \u306e\u30b5\u30a4\u30ba\u3092\u6709\u3059\u308b. Figure 1B<\/strong> \u3082\u307e\u305f 5.0 pN, 10.0 pN \u304a\u3088\u3073 105 pN \u306e\u529b\u3067\u306e\u3053\u306e\u5927\u304d\u3055\u306e\u5909\u5316\u3092\u793a\u3057\u3066\u3044\u308b\uff0e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304a\u3088\u3073\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u9077\u79fb\u3092\u30b9\u30ad\u30e3\u30f3\u3059\u308b\u3068\u5e83\u7bc4\u56f2\u306e\u529b\u304c\u81ea\u7531\u7d50\u5408\u9396\u30e2\u30c7\u30eb\u306b\u9069\u5408\u3059\u308b\u30c7\u30fc\u30bf\u3092\u8a31\u5bb9\u3059\u308b\u3053\u3068\u304c\u5206\u304b\u308b (63<\/a>). \u305d\u306e\u8f2a\u90ed\u9577\u306f 25 nm \u3067\u3042\u308a, Kuhn \u9577\u306f 0.9 nm \u3067\u3042\u308b (Figure 2B<\/strong>, \u5b9f\u7dda). \u30b4\u30e0\u30d0\u30f3\u30c9\u306e\u7dda\u5f62\u5f3e\u6027\u3068\u306f\u5bfe\u7167\u7684\u306b\uff0c\u30dd\u30ea\u30de\u30fc\u306e\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u53cd\u52d5\u306f\u9ad8\u5ea6\u306b\u975e\u7dda\u5f62\u6027\u3067\u3042\u308a\uff0c\u751f\u7406\u5b66\u7684\u306a\u529b\u306e\u7bc4\u56f2\u3092\u8d85\u3048\u3066\u305d\u306e\u6700\u5927\u4f38\u5c55\u306e 50 % \u306b\u3082\u9054\u3059\u308b\uff0e\u3053\u306e\u9ad8\u3044\u529b\u306b\u304a\u3044\u3066\u306f\uff0c\u86cb\u767d\u8cea\u306e\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u30b9\u30c6\u30c3\u30d7\u30b5\u30a4\u30ba\u306f\u6f38\u8fd1\u7684\u306b\u8f2a\u90ed\u9577\u306b\u8fd1\u3065\u304f\uff0e<\/p>\n

\"\"<\/a>
Figure 2<\/strong>
Ig \u30c9\u30e1\u30a4\u30f3\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u3088\u3063\u3066\u767a\u751f\u3057\u305f\u6a5f\u68b0\u7684\u4ed5\u4e8b\u306f\uff0c\u30d5\u30a9\u30fc\u30b9\u30af\u30e9\u30f3\u30d7\u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u3092\u7528\u3044\u3066\u5b9a\u91cf\u53ef\u80fd\u3067\u3042\u308b<\/strong>
(A)<\/strong> \u6a5f\u68b0\u7684\u5f35\u529b\u306e\u4e0b\u3067\u306e Ig \u30c9\u30e1\u30a4\u30f3\u306e\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\uff0c\u518d\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0. 6 pN \u307e\u3067\u306e\u727d\u5f15\u529b\u306e\u4e0b\u3067\u306f, Ig \u30c9\u30e1\u30a4\u30f3\u306f\uff0c\u30dd\u30ea\u30de\u30fc\u7269\u7406\u5b66\u306e\u30b9\u30b1\u30fc\u30ea\u30f3\u30b0\u5247\u306b\u5f93\u3044\uff0c\u5e73\u8861\u72b6\u614b\u3067\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304a\u3088\u3073\u518d\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3057\uff0c\u7d04 13 nm \u4f38\u9577\u307e\u305f\u306f\u77ed\u7e2e\u3059\u308b. (B)<\/strong> \u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u30fb\u518d\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u9593\u306b I10 \u304a\u3088\u3073 I91 \u306e\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u306e\u529b\u306e\u95a2\u6570\u3068\u3057\u3066\u6e2c\u5b9a\u3055\u308c\u305f\u30b9\u30c6\u30c3\u30d7\u30b5\u30a4\u30ba\u306e\u5927\u304d\u3055\uff0e\u30b9\u30c6\u30c3\u30d7\u30b5\u30a4\u30ba\u306e\u4f9d\u5b58\u6027\u306f, Kuhn \u9577 lk = 0.9 nm, \u8f2a\u90ed\u9577 Lc = 25 nm \u306e\u81ea\u7531\u7d50\u5408\u9396\u30e2\u30c7\u30eb\uff08\u5b9f\u7dda\uff09\u3067\u3088\u304f\u8a18\u8ff0\u3067\u304d\u308b\uff0e (C)<\/strong> \u6240\u4e0e\u306e\u727d\u5f15\u529b\u3067\uff0c\u6298\u308a\u7573\u307e\u308c\u305f\u30c9\u30e1\u30a4\u30f3\u306e\u5e73\u8861\u5206\u753b\uff08\u9752\u3044\u5186\uff09\u304b\u3089\u8a08\u7b97\u3055\u308c\u305f, Ig \u30c9\u30e1\u30a4\u30f3\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u78ba\u7387\uff0e\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u306f, 4 pN \u306e\u4f4e\u3044\u529b\u3067\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3057, 10 pN \u306e\u9ad8\u3044\u529b\u3067\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\uff0e\u305d\u306e\u30c7\u30fc\u30bf\u306f, 6 pN \u307e\u3067\u306e\u4e2d\u9593\u70b9\u3092\u6709\u3059\u308b\u30b7\u30b0\u30e2\u30a4\u30c9\u95a2\u6570\uff08\u5b9f\u7dda\uff09\u306b\u3088\u3063\u3066\u3088\u304f\u8868\u3059\u3053\u3068\u304c\u3067\u304d\u308b\uff0e (D)<\/strong> \u30bf\u30a4\u30c1\u30f3 Ig\u30c9\u30e1\u30a4\u30f3\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u3088\u308a\u751f\u6210\u3055\u308c\u308b\u4e88\u60f3\u3055\u308c\u308b\u4ed5\u4e8b\u306f\uff0c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u78ba\u7387\uff08\u9752\u7dda\uff09\u3068\u3001\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ce\u7e2e\u306b\u3088\u3063\u3066\u884c\u308f\u308c\u305f\u4ed5\u4e8b\uff08\u8d64\u7dda\uff09\u3068\u306e\u7a4d\u3068\u3057\u3066\u5f97\u3089\u308c\uff0c\u30b9\u30c6\u30c3\u30d7\u30b5\u30a4\u30ba\u306b\u9069\u5408\u3059\u308b\u3088\u3046\u7528\u3044\u305f\u81ea\u7531\u7d50\u5408\u9396\u30e2\u30c7\u30eb\u306e\u6570\u5024\u7a4d\u5206\u304b\u3089\u5f97\u3089\u308c\u308b\uff0e\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u306f 5.7 pN \u3067 46 zJ \u306e\u30d4\u30fc\u30af\u4ed5\u4e8b\u3092\u751f\u6210\u3057\uff0c\u305d\u308c\u306f\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306b\u3088\u308a\u4f9b\u7d66\u3055\u308c\u308b\u30a8\u30cd\u30eb\u30ae\u30fc\u306b\u5339\u6575\u3059\u308b\uff0e<\/figcaption><\/figure>\n

\u3000\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u306e\u305d\u308c\u305e\u308c\u304c\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3059\u308b\u969b\u306e\u7387\u306f\u5927\u5e45\u306b\u7570\u306a\u308b\uff0e\u305d\u306e\u305f\u3081\u751f\u7406\u7684\u306a\u4f38\u5c55\u4e2d\u306b\u306f\uff0c\u5168\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u306e\u4e00\u90e8\u306e\u307f\u304c\u4f38\u5c55\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b. I-band \u304b\u3089\u63a1\u53d6\u3057\u305f 8 \u672c\u8155\u306e Ig \u30c9\u30e1\u30a4\u30f3\u306e\u91ce\u751f\u578b\u30bb\u30b0\u30e1\u30f3\u30c8\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u3066\u307f\u308b\u3068\uff0c\u30bf\u30a4\u30c1\u30f3\u5185\u90e8\u306e\u6a5f\u68b0\u7684\u968e\u5c64\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\uff0e\u8fd1\u4f4d I-band \u5185\u90e8\u306e Ig \u30c9\u30e1\u30a4\u30f3\u306f Z \u76e4\u306b\u8fd1\u3044\u307b\u3069\uff0c\u9060\u4f4d I-band Ig \u30c9\u30e1\u30a4\u30f3\u3088\u308a\u3082\u306f\u308b\u304b\u306b\u5c0f\u3055\u3044\u529b\u3067\u4f38\u5c55\u3057\uff0c\u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306b\u8fd1\u3044 (36<\/a>). \u3086\u3048\u306b, I91 \u304c 10 pN \u3067\u4f38\u5c55\u3059\u308b\u306e\u306b\u6570\u6642\u9593\u3092\u8981\u3059\u308b\u306b\u3082\u304b\u304b\u308f\u3089\u305a (43,65), \u8fd1\u4f4d Ig \u30c9\u30e1\u30a4\u30f3 10 (I10) \u306f\u306f\u308b\u304b\u306b\u65e9\u304f\u4f38\u5c55\u3059\u308b\uff0eI10 \u3067\u306e\u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u5b9f\u9a13\u3067\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u306e\u306f, 6.0 pN \u306e\u529b\u3067\u306f, 20 \u5206\u9593\u3067\u7d04 66 \u56de\u306e\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u30a4\u30d9\u30f3\u30c8\u304c\u767a\u751f\u3057\uff0c\u305d\u308c\u305e\u308c\u306e\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u30a4\u30d9\u30f3\u30c8\u9593\u306b\u306f 15 \u79d2\u9593\u304c\u3042\u308b\u3068\u3044\u3046\u3053\u3068\u3067\u3042\u308b\uff0e\u305d\u308c\u3086\u3048 10 pN \u306b\u304a\u3044\u3066\u306f, I10 \u306f\u6570\u79d2\u9593\u3067\u4f38\u5c55\u3059\u308b\u3068\u63a8\u6e2c\u3055\u308c\u308b\u304c\uff0c\u3053\u308c\u306f\u5b9f\u9a13\u3067\u691c\u8a3c\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\uff0e\u91ce\u751f\u578b\u306e I4-I11 \u30bb\u30b0\u30e1\u30f3\u30c8\u3092\u7528\u3044\u305f\u5b9f\u9a13\u3067 5.0-6.6 pN \u9593\u306e\u529b\u3067\u306f\uff0c\u7d20\u65e9\u3044\u4f38\u5c55\u30a4\u30d9\u30f3\u30c8\u304c\u89b3\u6e2c\u3055\u308c\uff0c\u305d\u308c\u3086\u3048\u6a5f\u68b0\u7684\u5b89\u5b9a\u6027\u306f\u4f4e\u3044\u3068\u3055\u308c\u305f\uff0e\u3053\u308c\u306f\u8fd1\u4f4d Ig \u30c9\u30e1\u30a4\u30f3\u304c\u751f\u7406\u7684\u306a\u529b\u306e\u7bc4\u56f2\u3067\u5bb9\u6613\u306b\u4f38\u5c55\u3059\u308b\u3068\u3044\u3046\u8996\u70b9\u3092\u652f\u6301\u3057\u3066\u3044\u308b (10<\/a>). <\/p>\n

\u3000\u30bf\u30a4\u30c1\u30f3\u30c9\u30e1\u30a4\u30f3\u306e\u4f38\u5c55\u7d4c\u8def\u306b\u95a2\u3057\u3066\u306f\u8c4a\u5bcc\u306a\u60c5\u5831\u304c\u3042\u308b\u306b\u3082\u304b\u304b\u308f\u3089\u305a\uff0c\u6298\u308a\u305f\u305f\u307f\u7d4c\u8def\u306e\u89b3\u5bdf\u306f\u3055\u3089\u306b\u898b\u3064\u304b\u308a\u306b\u304f\u304f, force clamp \u6cd5\u306e\u3088\u3046\u306a\u6280\u8853\u306e\u767a\u5c55\u3092\u5f85\u305f\u306d\u3070\u306a\u3089\u306a\u304b\u3063\u305f (58<\/a>). \u305d\u306e force clamp \u6cd5\u306b\u3088\u308a\uff0c\u30e9\u30f3\u30c0\u30e0\u30b3\u30a4\u30eb\u304b\u3089\u81ea\u7136\u306a\u6298\u308a\u305f\u305f\u307f\u3078\u306e\u86cb\u767d\u8cea\u306e\u5f62\u614b\u306e\u5909\u5f62\u306e\u9593\u306b\uff0c\u4e09\u3064\u306e\u7570\u306a\u308b\u9077\u79fb\u304c\u3042\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f (34<\/a>). \u305d\u306e\u7d50\u679c\uff0c\u5f93\u6765\u306e\u4e00\u62ec\u6cd5\u3092\u7528\u3044\u3066\u898b\u3089\u308c\u305f\u306e\u3068\u306f\u5168\u304f\u7570\u306a\u308b\u56f3\u304c\u63cf\u304b\u308c\u308b\u3053\u3068\u3068\u306a\u3063\u305f\uff0e\u6700\u521d\u306b\uff0c\u3042\u308b\u86cb\u767d\u8cea\u306f\u529b\u306e\u5909\u5316\u306b\u5bfe\u5fdc\u3057\u3066\u529b\u306e\u4e0b\u3067\u5f3e\u6027\u53ce\u7e2e\u529b\u3092\u7d4c\u9a13\u3057\u3066\u4f38\u5c55\u3059\u308b. Figure 1B<\/strong> \u3092\u53c2\u7167\u3055\u308c\u305f\u3044\uff0e\u529b\u304c\u6e1b\u5f31\u3059\u308b\u3068\u30dd\u30ea\u30de\u30fc\u5185\u306e\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u304c\u5927\u5e45\u306b\u5897\u5927\u3057\uff0c\u86cb\u767d\u8cea\u304c\u6298\u308a\u305f\u305f\u307e\u308c\u308b\u3088\u3046\u306b\u3059\u308b\u306b\u9055\u3044\u306a\u3044\uff0e\u3053\u306e\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u3092\u9664\u53bb\u3059\u308b\u306b\u306f\u30a8\u30cd\u30eb\u30ae\u30fc\u969c\u58c1\u3092\u6a2a\u65ad\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\uff0c\u305d\u306e\u969c\u58c1\u306f\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u969c\u58c1\u3068\u3057\u3066\u77e5\u3089\u308c\u308b\u727d\u5f15\u529b\u306b\u3088\u308a\u4f5c\u3089\u308c\u308b (66<\/a>). \u3053\u306e\u9077\u79fb\u306f\uff0c\u30e2\u30eb\u30c6\u30f3\u30fb\u30b0\u30ed\u30d3\u30e5\u30fc\u30eb\u9077\u79fb\u3078\u306e\u6298\u308a\u305f\u305f\u307f\u3068\u3057\u3066\u77e5\u3089\u308c\u3066\u304a\u308a\uff0c\u758e\u6c34\u6027\u6b8b\u57fa\u306e\u86cb\u767d\u8cea\u30b3\u30a2\u3078\u306e\u9694\u96e2\u306b\u3088\u308a\u99c6\u52d5\u3055\u308c\uff0c\u305d\u306e\u7d50\u679c Figure 1B<\/strong> \u306b\u793a\u3059\u3088\u3046\u306b\u4e0b\u5411\u304d\u306e\u6bb5\u968e\u3068\u306a\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b\uff0e\u914d\u7f6e\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u306e\u3055\u3089\u306a\u308b\u6e1b\u5c11\u306f\uff0c\u30e2\u30eb\u30c6\u30f3\u30fb\u30b0\u30ed\u30d3\u30e5\u30fc\u30eb\u304b\u3089\u81ea\u7136\u72b6\u614b\u3078\u306e\u767a\u5c55\u306e\u904e\u7a0b\u3092\u901a\u3058\u3066\u9054\u6210\u3055\u308c\u308b\uff0e\u305d\u308c\u306f\u6a5f\u68b0\u7684\u30af\u30e9\u30f3\u30d7\u306b\u304a\u3051\u308b\u6c34\u7d20\u7d50\u5408\u306e\u5f62\u6210\u3092\u901a\u3058\u3066\u306a\u3055\u308c\u308b\u3088\u3046\u3067\u3042\u308b\uff0e\u3053\u306e\u30e2\u30eb\u30c6\u30f3\u30fb\u30b0\u30ed\u30d3\u30e5\u30fc\u30eb\u306e\u81ea\u7136\u9077\u79fb\u306f\u86cb\u767d\u8cea\u672b\u7aef\u9593\u306e\u9577\u3055\u306e\u5fae\u5c0f\u5909\u5316\u3092\u5f15\u304d\u8d77\u3053\u3057\uff0c\u30e2\u30eb\u30c6\u30f3\u30fb\u30b0\u30ed\u30d3\u30e5\u30fc\u30eb\u3068\u81ea\u7136\u72b6\u614b\u3068\u306e\u9593\u3067\u5287\u7684\u306b\u6a5f\u68b0\u7684\u5b89\u5b9a\u6027\u304c\u7570\u306a\u308b\u3053\u3068\u3067\uff0c\u7570\u306a\u308b\u72b6\u614b\u304c\u691c\u51fa\u3055\u308c\u308b\u3053\u3068\u306b\u306a\u308b (67<\/a>). \u5316\u5b66\u7684\u5909\u6027\u3084 phi-value analysis \u306e\u4e00\u62ec\u6cd5\u306e\u7814\u7a76\u3067\u60f3\u5b9a\u3055\u308c\u308b\u3053\u306e\u53e4\u5178\u7684\u306a\u300c\u4e8c\u72b6\u614b\u300d\u30e2\u30c7\u30eb\u306f\uff0c\u4f38\u5c55\u3055\u308c\u6298\u308a\u305f\u305f\u307e\u308c\u305f\u30e2\u30eb\u30c6\u30f3\u30fb\u30b0\u30ed\u30d3\u30e5\u30fc\u30eb\u3084\uff0c\u5358\u5206\u5b50\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u5b9f\u9a13\u3067\u89b3\u6e2c\u3055\u308c\u308b\u81ea\u7136\u72b6\u614b\u3068\u306f\u76f8\u5bb9\u308c\u306a\u3044\uff0e\u5358\u5206\u5b50\u539f\u5b50\u9593\u529b\u5206\u5149\u6cd5\u306f\uff0c\u3053\u308c\u3089\u5f93\u6765\u306e\u624b\u6cd5\u3088\u308a\u3082\uff0c\u3053\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u7d4c\u8def\u306b\u3064\u3044\u3066\u3088\u308a\u591a\u304f\u306e\u8a73\u7d30\u3092\u3082\u305f\u3089\u3057\u3066\u304f\u308c\u308b\uff0e<\/p>\n

Protein folding performs mechanical work<\/h3>\n

\u3000Force-clamp \u539f\u5b50\u9593\u529b\u9855\u5fae\u93e1\u306e\u767a\u660e\u4ee5\u524d\u306b\u306f\uff0c\u529b\u306e\u4e0b\u3067\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u8a08\u6e2c\u306f\u975e\u5e38\u306b\u5358\u7d14\u306a\u3082\u306e\u3057\u304b\u306a\u304b\u3063\u305f (33<\/a>). \u3053\u308c\u3089\u306e\u5b9f\u9a13\u3067\u306f\uff0c\u516b\u3064\u306e\u8155\u306e\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u3092\u542b\u3080\u86cb\u767d\u8cea\u306e\u69cb\u6210\u304c\uff0c\u4e00\u5b9a\u901f\u5ea6\u3067\u306e AFM \u30ab\u30f3\u30c1\u30ec\u30d0\u30fc\u306e\u727d\u5f15\u306b\u3088\u308b\u6700\u521d\u306e\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3068\u306a\u3063\u305f\uff0e\u5f15\u304d\u7d9a\u3044\u3066\u30ab\u30f3\u30c1\u30ec\u30d0\u30fc\u5148\u7aef\u304c\u8a66\u6599\u8868\u9762\u304b\u3089\u306e\u529b\u3067\u3054\u304f\u77ed\u3044\u8ddd\u96e2\uff08\u6570\u5341\u30ca\u30ce\u30e1\u30fc\u30c8\u30eb\uff09\u4e0b\u304c\u308a\uff0c\u63a5\u89e6\u306a\u3057\u306b\u3054\u304f\u77ed\u6642\u9593\u305d\u3053\u306b\u3068\u3069\u307e\u308b\uff0e\u3053\u306e\u904e\u7a0b\u304c\u86cb\u767d\u8cea\u306e residual force \u306e\u6b8b\u7559\u52b9\u679c\u3067\u3042\u308b\uff0e\u30ab\u30f3\u30c1\u30ec\u30d0\u30fc\u304c\u518d\u5ea6\u727d\u5f15\u3055\u308c\u308b\u3053\u3068\u3067\uff0c\u305d\u306e\u6298\u308a\u305f\u305f\u307e\u308c\u305f\u30c9\u30e1\u30a4\u30f3\u6570\u304c\u8a08\u6e2c\u3067\u304d\u308b\uff0e\u305d\u308c\u3089\u306e\u5b9f\u9a13\u304b\u3089\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u306e\u306f\uff0c\u518d\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u7387\u304c\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c9\u3057\u3066\u3044\u308b\u86cb\u767d\u8cea\u306e\u5f1b\u7de9\u306e\u7a0b\u5ea6\u306b\u5f37\u304f\u4f9d\u5b58\u3059\u308b\u3068\u3044\u3046\u3053\u3068\u3067\u3042\u308a (33<\/a>), \u518d\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u7387\u306f residual force \u306b\u6025\u6fc0\u306b\u4f9d\u5b58\u3059\u308b\u3068\u306e\u30a8\u30d3\u30c7\u30f3\u30b9\u304c\u5f97\u3089\u308c\u305f\uff0e\u3057\u304b\u3057\uff0c\u305d\u308c\u3089\u521d\u671f\u306e\u5b9f\u9a13\u306b\u304a\u3044\u3066\u306f\uff0c\u86cb\u767d\u8cea\u304c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3059\u308b\u529b\u306e\u4e0b\u3067\u306f\uff0c\u76f4\u63a5\u8a08\u6e2c\u304c\u3067\u304d\u306a\u304b\u3063\u305f\uff0eForce-clamp AFM \u306e\u767a\u5c55\u306f\uff0c\u4e16\u754c\u3067\u521d\u3081\u3066\u6a5f\u68b0\u7684\u306a\u529b\u306e\u4e0b\u3067\u306e\u86cb\u767d\u8cea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u5b8c\u5168\u3067\u92ed\u3044\u76f4\u63a5\u89b3\u5bdf\u3092\u3082\u305f\u3089\u3057\u3066\u304f\u308c\u305f (34<\/a>). \u30e6\u30d3\u30ad\u30c1\u30f3\u30c9\u30e1\u30a4\u30f3\u8155\u304b\u3089\u306a\u308b\u86cb\u767d\u8cea\u69cb\u6210\u3092\u7528\u3044\u3066, \u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c9\u3057\u305f\u86cb\u767d\u8cea\u3092\u727d\u5f15\u3059\u308b\u529b\u3092\u300c\u6025\u505c\u6b62\u300d\u3059\u308b\u3068\uff0c\u305d\u306e\u86cb\u767d\u8cea\u306f\u307e\u305a\u5f3e\u6027\u53ce\u7e2e\u3057\uff0c\u6b21\u3044\u3067\u9045\u5ef6\u306e\u5f8c\uff0c\u727d\u5f15\u529b\u306b\u62b5\u6297\u3057\u3066\u6a5f\u68b0\u7684\u4ed5\u4e8b\u3092\u884c\u3044\uff0c\u5b8c\u5168\u306b\u6298\u308a\u305f\u305f\u307e\u308c\u305f\u9577\u3055\u306b\u77ed\u7e2e\u3059\u308b\u3053\u3068\u3092 Fernandez \u3068 Li \u304c\u8a3c\u660e\u3057\u305f\uff0e\u529b\u306e\u6025\u505c\u6b62\u5f8c\uff0c\u7d9a\u3044\u3066\u9ad8\u3044\u529b\u306e\u30d1\u30eb\u30b9\u304c\u6298\u308a\u305f\u305f\u307e\u308c\u305f\u30c9\u30e1\u30a4\u30f3\u306e\u6307\u7d0b\u3092\u660e\u3089\u304b\u306b\u3057\uff0c\u3053\u308c\u3089\u306e\u6298\u308a\u305f\u305f\u307f\u306e\u8ecc\u8de1\u306f\uff0c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u904e\u7a0b\u304c\u771f\u5b9f\u306e\u89b3\u5bdf\u3067\u3042\u308b\u3053\u3068\u3092\u6559\u3048\u3066\u304f\u308c\u308b\uff0e\u3088\u308a\u77ed\u6642\u9593\u306e\u6025\u505c\u6b62\u3067\u306e\u4f3c\u305f\u5b9f\u9a13\u3067\u306f\u6a5f\u68b0\u7684\u306b\u8106\u5f31\u306a\u72b6\u614b\u306e\u5b58\u5728\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\uff0e\u305d\u3053\u3067\u306f\uff0c\u5b8c\u5168\u306b\u6298\u308a\u305f\u305f\u307e\u308c\u305f\u86cb\u767d\u8cea\u306e\u524d\u99c6\u4f53\u3068\u3057\u3066\u4fdd\u5b58\u3055\u308c\uff0c\u30e2\u30eb\u30c6\u30f3\u30fb\u30b0\u30ed\u30d3\u30e5\u30fc\u30eb\u72b6\u614b\u3092\u30b3\u30d4\u30fc\u3057\u305f\u72b6\u6cc1\u306b\u4f3c\u3066\u3044\u308b (67<\/a>,68<\/a>,69<\/a>). 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AFM \u3068\u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u6cd5\u3067\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u8ecc\u9053\u306e\u5f62\u304c\u7570\u306a\u308b\u7406\u7531\u306f\uff0c\u3059\u3079\u3066\u306e\u5358\u5206\u5b50\u539f\u5b50\u9593\u529b\u5206\u5149\u6cd5\u306e\u5b9f\u9a13\u306b\u304a\u3044\u3066\uff0c\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u306e\u529b\u5b66\u304c\u30c6\u30b6\u30fc\u4f9d\u5b58\u6027\u3067\u3042\u308b\u3068\u3044\u3046\u3053\u3068\u3067\u3042\u308b (73<\/a>). \u305d\u306e\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u306f\u63a2\u91dd\u304c\u63a5\u89e6\u3059\u308b\u307b\u3069\u901f\u304f\u52d5\u304f\u3053\u3068\u304c\u3067\u304d\u308b\u306e\u307f\u3067\uff0c\u727d\u5f15\u3059\u308b\u65b9\u5411\u306e\u9396\u306e\u529b\u5b66\u306f\uff0c\u63a2\u91dd\u3068\u540c\u3058\u6709\u52b9\u306a\u62e1\u6563\u4fc2\u6570\u3092\u6709\u3059\u308b\uff0e\u4e09\u89d2\u5f62\u306e\u30ab\u30f3\u30c1\u30ec\u30d0\u30fc\u306f\u901a\u5e38\u5358\u5206\u5b50\u306e\u5b9f\u9a13\u3067\u7528\u3044\u3089\u308c\uff0c\u9577\u3055\u3068\u5e45\u304c\u305d\u308c\u305e\u308c 310 µm \u304a\u3088\u3073 20 µm \u3067\u3042\u308a\uff0c\u62e1\u6563\u4fc2\u6570\u306f 1,200 nm2<\/sup> \u3068\u6e2c\u5b9a\u3055\u308c\u3066\u3044\u308b (73<\/a>). \u30ab\u30f3\u30c1\u30ec\u30d0\u30fc\u306e\u62e1\u6563\u4fc2\u6570\u3092\u7528\u3044\u305f\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u30d6\u30e9\u30a6\u30f3\u904b\u52d5\u529b\u5b66\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u306f\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u8ecc\u9053\u306e\u518d\u73fe\u6027\u3092\u53ef\u80fd\u3068\u3057, force-clamp AFM \u3092\u7528\u3044\u305f\u3082\u306e\u3068\u8fd1\u4f3c\u3057\u3066\u3044\u305f (66<\/a>,74<\/a>). \u5bfe\u7167\u7684\u306b\uff0c\u76f4\u5f84 2.8 µm \u306e\u81ea\u7531\u6d6e\u52d5\u7403\u4f53\u7c92\u5b50\u306f 175,000 nm2<\/sup> \u307e\u3067\u306e\u62e1\u6563\u4fc2\u6570\u3092\u6301\u3064\uff0e\u78c1\u6c17\u30d3\u30fc\u30ba\u304c\u8a66\u6599\u8868\u9762\u3068\u8fd1\u63a5\u3057\u3066\u3044\u3066\u305d\u306e\u5024\u3092\u82e5\u5e72\u6e1b\u5f31\u3059\u308b\u306b\u3082\u304b\u304b\u308f\u3089\u305a\u3067\u3042\u308b (75<\/a>,76<\/a>). \u3053\u308c\u306f\u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u3067\u898b\u3089\u308c\u308b\u6298\u308a\u305f\u305f\u307f\u8ecc\u9053\u306e\u9055\u3044\u306b\u5bc4\u4e0e\u3057\u3066\u3044\u308b\uff0e\u3053\u3053\u3067\u6ce8\u610f\u304c\u5fc5\u8981\u3067\u3042\u308b\uff0e\u7f8e\u3057\u3044\u6bb5\u968e\u7684\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u8ecc\u9053\u304c\u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u6cd5\u3067\u89b3\u6e2c\u3055\u308c\uff0c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u30a8\u30cd\u30eb\u30ae\u30fc\u306e\u98a8\u666f (77<\/a>) \u304a\u3088\u3073\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u4ed5\u4e8b (10<\/a>) \u306e\u753b\u50cf\u304c\u3088\u308a\u826f\u3044\u89e3\u50cf\u5ea6\u3067\u5f97\u3089\u308c\u305f\u304b\u3089\u3068\u8a00\u3063\u3066\uff0c\u305d\u308c\u304c\u751f\u4f53\u5185\u3067\u306e\u30bf\u30a4\u30c1\u30f3\u86cb\u767d\u8cea\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u771f\u5b9f\u306e\u62e1\u6563\u306e\u3075\u308b\u307e\u3044\u3092\u8868\u3057\u3066\u3044\u308b\u308f\u3051\u3067\u306f\u306a\u3044\uff0e\u30bf\u30a4\u30c1\u30f3\u304c Z \u76e4\u3068 M-line \u3068\u3044\u3046\u5de8\u5927\u306a\u5206\u5b50\u69cb\u9020\u306b\u4fc2\u7559\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304b\u3089\uff0c\u307e\u305f\uff0c\u30b7\u30e3\u30da\u30ed\u30f3\u3092\u542b\u3080\u6df7\u96d1\u3057\u305f\u74b0\u5883\u3067\u52d5\u4f5c\u3059\u308b\u3053\u3068\u304b\u3089\uff0c\u751f\u4f53\u5185\u3067\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ce\u7e2e\u306f AFM \u3067\u89b3\u6e2c\u3055\u308c\u308b\u3088\u3046\u306a\u6ed1\u3089\u304b\u306a\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ce\u7e2e\u306e\u3088\u3046\u306b\u3082\u3063\u3068\u62e1\u6563\u306e\u5236\u7d04\u3055\u308c\u305f\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u3075\u308b\u307e\u3044\u306b\u4f3c\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b (34<\/a>,74<\/a>). <\/p>\n

\u3000\u6211\u3005\u306f\u63d0\u6848\u3059\u308b\uff0e\u6b63\u5e38\u306a\u7b4b\u6d3b\u52d5\u6642\u306e\u30bf\u30a4\u30c1\u30f3\u3078\u306e\u8ca0\u8377\u306e\u751f\u7406\u7684\u306a\u529b\u306e\u7bc4\u56f2\u3068\u306f\uff0c\u6298\u308a\u305f\u305f\u307f\u3068\u4f38\u5c55\u306e\u5e73\u8861\u72b6\u614b\u3067 Ig \u30c9\u30e1\u30a4\u30f3\u304c\u5b58\u5728\u3059\u308b\u7bc4\u56f2\u3092\u8d85\u3048\u308b\u529b\u306e\u7bc4\u56f2\u3067\u3042\u308b\u3068\uff0e\u3053\u308c\u306f\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u78ba\u7387\u3092\u901a\u3058\u3066\u5b9a\u91cf\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\uff0c\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u304c\u4efb\u610f\u306e\u529b\u3067\u6298\u308a\u305f\u305f\u307e\u308c\u308b\u6642\u9593\u306e\u5272\u5408\u3092\u8a08\u6e2c\u3059\u308b\u3082\u306e\u3067\u3042\u308b\uff0e\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3 I10 \u304a\u3088\u3073 I91 \u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u78ba\u7387\u3092\u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u306e\u539f\u5b50\u9593\u529b\u5206\u5149\u6cd5\u3067\u8a08\u6e2c\u3057, Figure 2C<\/strong> \uff08\u9752\u4e38\uff09\u306b\u8981\u7d04\u3057\u3066\u3042\u308a\uff0c\u30b7\u30b0\u30e2\u30a4\u30c9\u95a2\u6570\u306b\u9069\u5408\u3057\u3066\u304a\u308a\uff0c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53cd\u5fdc\u306e midpoint \u3068 steepness \u3092\u8a18\u8ff0\u3057\u3066\u3042\u308b (\u9752\u7dda, Figure 2C<\/strong>, Figure 2D<\/strong>). \u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u304c 4 pN \u4ee5\u4e0b\u306e\u529b\u3067\u5b8c\u5168\u306b\u6298\u308a\u305f\u305f\u307e\u308c, 10 pN \u3088\u308a\u5927\u304d\u3044\u529b\u3067\u306f\u6298\u308a\u305f\u305f\u307e\u308c\u306a\u3044\u3053\u3068\u306f\u660e\u3089\u304b\u3067\u3042\u308b\uff0e\u73fe\u5728\u3067\u306f\u3053\u306e\u529b\u306e\u7bc4\u56f2\u306f 15 pN \u307e\u3067\u62e1\u5927\u3057\u3066\u3044\u308b\u3068\u306e\u6839\u62e0\u304c\u3042\u308b\uff0e\u30bf\u30a4\u30c1\u30f3\u306e\u3053\u306e I-band \u9818\u57df\u306f\u30b7\u30b9\u30c6\u30a4\u30f3\u304c\u8c4a\u5bcc\u3067 (78<\/a>,79<\/a>), \u3053\u308c\u3089\u306e\u591a\u304f\u306e\u6b8b\u57fa\u306f\u30b8\u30b9\u30eb\u30d5\u30a3\u30c9\u7d50\u5408\u3092\u5f62\u6210\u3057\uff0c\u30dd\u30ea\u30de\u30fc\u529b\u5b66\u3092\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u72b6\u614b\u306b\u5909\u3048\u308b\uff0e\u305d\u306e\u7d50\u679c\uff0c\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u304c 15 pN \u307e\u3067\u306e\u3088\u308a\u9ad8\u3044\u529b\u3067\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c9\u3057\u3084\u3059\u304f\u306a\u308a\uff0c\u6298\u308a\u305f\u305f\u307f\u72b6\u614b\u306b\u304a\u3051\u308b\u6a5f\u68b0\u7684\u5b89\u5b9a\u6027\u3092\u3088\u308a\u5931\u308f\u305b\u3084\u3059\u304f\u3059\u308b\u52b9\u679c\u304c\u3042\u308b (80<\/a>). <\/p>\n

\u3000Force-clamp \u5206\u5149\u6cd5\u304c\u4ed8\u52a0\u7684\u306b\u53ef\u80fd\u306b\u3057\u305f\u5358\u7d14\u306a\u5b9a\u91cf\u5316\u3068\u3057\u3066\uff0c\u30bf\u30a4\u30c1\u30f3\u30c9\u30e1\u30a4\u30f3\u304c\u9069\u7528\u3055\u308c\u305f\u529b\u306b\u6297\u3057\u3066\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3057\u3066\u5b9f\u884c\u3059\u308b\u53ce\u7e2e\u306e\u4ed5\u4e8b\u91cf\u304c\u3042\u308b\uff0e\u3044\u304b\u306a\u308b\u529b\u3092\u4e0e\u3048\u3066\u3082\uff0c\u30b7\u30b9\u30c6\u30e0\u4e0a\u307e\u305f\u306f\u30b7\u30b9\u30c6\u30e0\u306b\u3088\u308a\u306a\u3055\u308c\u308b\u53ce\u7e2e\u306e\u4ed5\u4e8b\u91cf\u306f\uff0c\u529b\u3068\u30b9\u30c6\u30c3\u30d7\u30b5\u30a4\u30ba\u306e\u7a4d\u3067\u3042\u308b. I10 \u304a\u3088\u3073 I91 \u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u3088\u308a\u306a\u3055\u308c\u308b\u4ed5\u4e8b\u306f Figure 2D<\/strong> \u306e\u8d64\u3044\u66f2\u7dda\u3067\u793a\u3057\u305f\u304c\uff0c\u529b\u306b\u5bfe\u5fdc\u3059\u308b\u81ea\u7531\u7d50\u5408\u9396\u66f2\u7dda\u306e\u7a4d\u5206\u3067\u3042\u308b\uff0e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u78ba\u7387\u3067\u306a\u3055\u308c\u305f\u53ce\u7e2e\u4ed5\u4e8b\u3092\u4ee3\u8868\u3059\u308b\u4e57\u7b97\u66f2\u7dda\u306f\uff0c\u4e0e\u3048\u3089\u308c\u305f\u529b\u3067\u306e\u86cb\u767d\u8cea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u3088\u308b\u30a8\u30cd\u30eb\u30ae\u30fc\u56de\u5fa9\u306e\u671f\u5f85\u5024\u3092\u4e0e\u3048\u3066\u304f\u308c\u308b (\u9ed2\u306e\u66f2\u7dda, Figure 2D<\/strong>). \u3053\u306e\u5206\u5e03\u304c\u793a\u3059\u3068\u3053\u308d\u306f\uff0c\u30bf\u30a4\u30c1\u30f3 Ig \u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304c, 5.7 pN \u306e\u8ca0\u8377\u306b\u5bfe\u3057\u3066 47 zJ \u3068\u671f\u5f85\u3055\u308c\u308b\u6700\u5927\u306e\u4ed5\u4e8b\u3092\u767a\u751f\u3055\u305b\u308b\u3068\u3044\u3046\u3053\u3068\u3067\u3042\u308b\uff0e10 pN \u306b\u8fd1\u3044\u9ad8\u3044\u8ca0\u8377\u3067\u306f\uff0c\u30a8\u30cd\u30eb\u30ae\u30fc\u56de\u5fa9\u306f\u6700\u5927\u5316\u3059\u308b\u304c\uff0c\u30c9\u30e1\u30a4\u30f3\u306e\u6298\u308a\u305f\u305f\u307f\u983b\u5ea6\u306f\u6e1b\u5c11\u3059\u308b\uff0e\u4ee3\u308f\u308a\u306b\uff0c\u8efd\u3044\u8ca0\u8377\u306b\u304a\u3044\u3066\u306f\u30a8\u30cd\u30eb\u30ae\u30fc\u56de\u5fa9\u306f\u6700\u5c0f\u5316\u3059\u308b\uff0e\u3068\u3044\u3046\u306e\u306f\uff0c\u86cb\u767d\u8cea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u30b9\u30c6\u30c3\u30d7\u30b5\u30a4\u30ba\u304c\u5c0f\u3055\u304f\uff0c\u307b\u3068\u3093\u3069\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u304c\u5f3e\u6027\u53ce\u7e2e\u3067\u6d88\u8cbb\u3055\u308c\u3066\u3057\u307e\u3046\u304b\u3089\u3067\u3042\u308b\uff0e\u3086\u3048\u306b\u6211\u3005\u306f\uff0c\u751f\u4f53\u5185\u3067\u5358\u4e00\u306e\u30bf\u30a4\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306b\u304b\u304b\u308b\u529b\u3092\u5b9a\u7fa9\u3067\u304d\u308c\u3070\uff0c\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3092\u901a\u3058\u3066\u84c4\u7a4d\u3055\u308c\u89e3\u653e\u3055\u308c\u308b\u30a8\u30cd\u30eb\u30ae\u30fc\u91cf\u3092\u4e88\u6e2c\u3067\u304d\u308b\uff0e<\/p>\n

How the three filaments work together<\/h3>\n

\u3000\u5358\u5206\u5b50\u306e\u5b9f\u9a13\u306f\uff0c\u86cb\u767d\u8cea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u7406\u8ad6\u306b\u3064\u3044\u3066\u7406\u89e3\u3059\u308b\u306e\u307f\u306a\u3089\u305a\uff0c\u5358\u4e00\u306e\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306b\u3088\u308b\u529b\u306e\u767a\u751f\u306e\u57fa\u790e\u306b\u3064\u3044\u3066\u7406\u89e3\u3059\u308b\u305f\u3081\u306e\u5f37\u56fa\u306a\u57fa\u76e4\u3092\u63d0\u4f9b\u3057\u3066\u304d\u305f\uff0e\u3053\u308c\u306f\u30bf\u30a4\u30c1\u30f3 Ig \u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u53ce\u7e2e\u7279\u6027\u3068\u30df\u30aa\u30b7\u30f3\u30d1\u30ef\u30fc\u30b9\u30c8\u30ed\u30fc\u30af\u3068\u306e\u9593\u306e\u516c\u5e73\u306a\u6bd4\u8f03\u3092\u610f\u5473\u3057\u3066\u3044\u308b\uff0e\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306e\u72ec\u594f\u307e\u305f\u306f\u5408\u594f\u306b\u304a\u3051\u308b\u5358\u5206\u5b50\u539f\u5b50\u9593\u529b\u5206\u5149\u6cd5\u306f, ATP \u3067\u99c6\u52d5\u3055\u308c\u308b\u30e2\u30fc\u30bf\u30fc\u304c\u5b9f\u884c\u3067\u304d\u308b\u5229\u7528\u53ef\u80fd\u306a\u4ed5\u4e8b\u91cf\u306b\u56fa\u6709\u306e\u5236\u9650\u304c\u3042\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u305f\uff0e\u4eee\u306b\u5341\u5206\u306b\u5927\u304d\u306a\u8ca0\u8377\u304c\u30e2\u30fc\u30bf\u30fc\u306b\u304b\u304b\u308b\u3068\uff0c\u305d\u306e\u30e2\u30fc\u30bf\u30fc\u51fa\u529b\u306f\u30bc\u30ed\u306b\u4f4e\u4e0b\u3057\u3066\u3057\u307e\u3046\uff0e\u81ea\u8ee2\u8eca\u3067\u5742\u9053\u3092\u767b\u308b\u6642\uff0c\u7269\u7406\u5b66\u306e\u3053\u306e\u4e00\u304b\u3051\u3089\u3092\u75db\u611f\u3055\u305b\u3089\u308c\u308b\uff0e\u521d\u671f\u306e\u7b4b\u8089\u306e\u30d1\u30a4\u30aa\u30cb\u30a2\u3067\u3042\u308b A. V. Hill \u306f\u3053\u306e\u52b9\u679c\u3092\u6349\u3048\u3066\u5f7c\u306e\u6709\u540d\u306a\u300cHill \u306e\u516c\u5f0f\u300d\u3068\u3057\u305f\uff0e\u3064\u307e\u308a\uff0c\u53ce\u7e2e\u901f\u5ea6\u3068\u8ca0\u8377\u3068\u306e\u9593\u306e\u53cc\u66f2\u7dda\u95a2\u4fc2\u3092\u8a18\u8ff0\u3057\u305f\u3082\u306e\u3067\u3042\u308b (81<\/a>). \u8a66\u9a13\u7ba1\u5185\u306e\u5b9f\u9a13\u304c\u660e\u3089\u304b\u306b\u3057\u305f\u3068\u3053\u308d\u3067\u306f\uff0c\u514d\u8377\u72b6\u614b\u306e\u7b4b\u8089\u306e\u901f\u5ea6\u306f\u6975\u3081\u3066\u901f\u3044\u304c\uff0c\u5c11\u3057\u3067\u3082\u8ca0\u8377\u3092\u304b\u3051\u308b\u3068, II \u578b\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306e\u6ed1\u8d70\u901f\u5ea6\u304c\u6025\u6fc0\u306b\u4f4e\u4e0b\u3057\u3066\u3057\u307e\u3046\uff0e\u9aa8\u683c\u7b4b\u306e\u30df\u30aa\u30b7\u30f3\u306e\u6a5f\u5e8f\u3092\u63a2\u7d22\u3059\u308b\u7cbe\u5bc6\u306a\u5b9f\u9a13\u3067\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u306e\u306f\uff0c\u7b49\u5c3a\u6027\u72b6\u614b\u4e0b\u3067\u30e2\u30fc\u30bf\u30fc 1 \u500b\u304c\u7523\u751f\u3059\u308b\u529b\u306e\u5e73\u5747\u5024\u306f 3.4 pN \u3067\u3042\u308b (26<\/a>). \u307e\u308c\u306b\uff0c\u3053\u306e\u30df\u30aa\u30b7\u30f3\u304c\u6301\u7d9a\u7684\u306b\u767a\u751f\u3055\u305b\u308b\u529b\u304c 6-7 pN \u306e\u7bc4\u56f2\u306b\u9077\u79fb\u3059\u308b\u3053\u3068\u304c\u3042\u308b\uff0e\u3055\u3089\u306b\u6700\u8fd1\u3067\u306f\uff0c\u5358\u5206\u5b50\u306e\u529b-\u901f\u5ea6\u95a2\u4fc2\u3092\u5b9a\u7fa9\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u3068\u3057\u3066\uff0c\u540c\u3058\u5b9f\u9a13\u304c 8 \u500b\u307e\u3067\u306e\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u3092\u675f\u306b\u3057\u3066\u5b9f\u65bd\u3055\u308c\u305f (Figure 3A<\/strong>, \u7dd1\u8272\u66f2\u7dda) (82<\/a>). \u3053\u306e\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306f\u3084\u306f\u308a 6.0 pN \u306e\u8ca0\u8377\u3092\u9069\u7528\u3059\u308b\u3068\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306e\u5909\u4f4d\u304c\u6b63\u5473\u30bc\u30ed\u306b\u306a\u3063\u3066\u3057\u307e\u3044\uff0c\u3053\u306e\u5206\u5b50\u30de\u30b7\u30f3\u306e\u5b9f\u884c\u306b\u306f\u4e0a\u9650\u5024\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u3092\u518d\u78ba\u8a8d\u3057\uff0c\u305d\u308c\u306f\u9ad8\u89e3\u50cf\u5ea6\u5149\u30d4\u30f3\u30bb\u30c3\u30c8\u6cd5\u3067\u3082\u518d\u78ba\u8a8d\u3055\u308c\u305f (29<\/a>). \u4ed6\u306b\u30ad\u30cd\u30b7\u30f3, V \u578b\u30df\u30aa\u30b7\u30f3\uff0c\u30c0\u30a4\u30cb\u30f3\u306e\u5931\u901f\u3059\u308b\u529b\u306f\u3059\u3079\u3066 6-7 pN \u306e\u7bc4\u56f2\u306b\u53ce\u307e\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u3066\u3044\u308b (83<\/a>,84<\/a>,85<\/a>). \u3053\u308c\u3089\u306e\u5b9f\u9a13\u306f force clamp \u6cd5\u3092\u7528\u3044\u3066\u5909\u63db\u5668\u306e\u5fdc\u7b54\u3092\u6e1b\u5f31\u3055\u305b\uff0c\u5206\u5b50\u30e2\u30fc\u30bf\u30fc\u306e\u52d5\u529b\u767a\u751f\u3092\u6700\u5927\u5316\u3057\u3066\u3044\u308b\uff0e\u3053\u308c\u3089\u306e\u30c7\u30fc\u30bf\u304b\u3089\u8ad6\u7406\u7684\u306b\u5c0e\u304b\u308c\u308b\u7d50\u679c\u3068\u306f, 6 pN \u306e\u529b\u3067\u306f II \u578b\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306f\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306b\u53ce\u7e2e\u306e\u4ed5\u4e8b\u3092\u767a\u751f\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u306a\u3044\u3068\u3044\u3046\u3053\u3068\u3067\u3042\u308b\uff0e\u5bfe\u7167\u7684\u306b\uff0c\u30bf\u30a4\u30c1\u30f3\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304c 6 pN \u306e\u8ca0\u8377\u306b\u304a\u3044\u3066\u30d4\u30fc\u30af\u30d1\u30ef\u30fc\u3092\u63d0\u4f9b\u3059\u308b\u3053\u3068\u3092\u6211\u3005\u306f\u793a\u3057\u305f\u304c\uff0c\u305d\u3053\u3067\u306f II \u578b\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306f\u5931\u901f\u3057\u3066\u3044\u305f (Figure 2D<\/strong>, Figure 3A<\/strong>) (10<\/a>). <\/p>\n

\"\"<\/a>
Figure 3<\/strong>
\u30bf\u30a4\u30c1\u30f3\u306f\uff0c\u7b4b\u8089\u304c\u30df\u30aa\u30b7\u30f3\u306e\u5931\u901f\u3059\u308b\u529b\u3092\u8d85\u3048\u308b\u8ca0\u8377\u3067\u50cd\u304f\u3053\u3068\u3092\u53ef\u80fd\u306b\u3059\u308b<\/strong>
(A)<\/strong> \u5358\u5206\u5b50\u7814\u7a76\u304b\u3089\u306e\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u3068\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u8ad6\u306e\u6bd4\u8f03: 8 \u500b\u307e\u3067\u306e\u30df\u30aa\u30b7\u30f3\u982d\u90e8\u304c\u5149\u30c8\u30e9\u30c3\u30d7\u4e2d\u306e\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u3092\u63a8\u9032\u3057\u305f\u5b9f\u9a13\u304b\u3089\u306e\u30c7\u30fc\u30bf\u306f\uff0c\u30df\u30aa\u30b7\u30f3\u304c 6 pN \u306e\u8ca0\u8377\u306b\u5bfe\u3057\u3066\u5931\u901f\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u3057\u3066\u3044\u308b (82<\/a>). \u305d\u306e\u30c7\u30fc\u30bf\u306f A.V.Hill \u306e\u516c\u5f0f\uff08\u7dd1\u8272\u306e\u66f2\u7dda\uff09\u306b\u5408\u81f4\u3059\u308b\uff0e\u3042\u308b\u3044\u306f\uff0c\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u306f 5.7 pN \u307e\u3067\uff08\u9752\u8272\u66f2\u7dda\u306e\u30d4\u30fc\u30af\uff09\u306e\u529b\u3067 46 zJ \u306e\u4ed5\u4e8b\u306e\u6700\u9069\u306a\u4f9b\u7d66\u3092\u9054\u6210\u3059\u308b\uff0e\u3057\u305f\u304c\u3063\u3066, 6-12 pN \uff08\u7070\u8272\u306e\u9670\u5f71\u90e8\u5206\uff09\u306e\u8ca0\u8377\u3067\u306f\uff0c\u30df\u30aa\u30b7\u30f3\u306e\u51fa\u529b\u304c\u30de\u30a4\u30ca\u30b9\u3067\u3042\u308b\u306e\u306b\u5bfe\u3057\u3066\uff0c\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306f\u6709\u7528\u306a\u53ce\u7e2e\u306e\u4ed5\u4e8b\u3092\u767a\u751f\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u308b. (B)<\/strong> \u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u3088\u3063\u3066\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u304c\u524d\u9032\u3059\u308b: (1) \u7b4b\u8089\u304c\u6d3b\u6027\u5316\u3055\u308c\u308b\u524d\u306b\u30bf\u30a4\u30c1\u30f3\u306f 12 pN \u306e\u53d7\u52d5\u7684\u8ca0\u8377\u5168\u4f53\u3092\u8ca0\u62c5\u3057\uff0c\u305d\u306e\u7d50\u679c, Ig \u30c9\u30e1\u30a4\u30f3\u306e\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304c\u8d77\u3053\u308b\uff0e (2) \u30ab\u30eb\u30b7\u30a6\u30e0\u304c\u653e\u51fa\u3055\u308c\u308b\u3068\uff0c\u30a2\u30af\u30c8\u30df\u30aa\u30b7\u30f3\u67b6\u6a4b\u304c\u5f62\u6210\u3055\u308c\u308b\u304c\uff0c\u305d\u306e\u30e2\u30fc\u30bf\u30fc\u306f\u5931\u901f\u3057\u3066\u3044\u308b\u305f\u3081, 6 pN \u306e\u529b\u3057\u304b\u767a\u751f\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u306a\u3044\uff0e\u3057\u304b\u3057\uff0c\u30e2\u30fc\u30bf\u30fc\u306b\u3088\u3063\u3066\u767a\u751f\u3057\u305f\u529b\u306f\u30bf\u30a4\u30c1\u30f3\u30c9\u30e1\u30a4\u30f3\u304c\u53d7\u3051\u308b\u8ca0\u8377\u3092 6 pN \u306b\u6e1b\u5c11\u3055\u305b\uff0c\u7d50\u679c\u3068\u3057\u3066 10 nm \u307e\u3067\u306e\u518d\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u30b9\u30c6\u30c3\u30d7\u3092\u3082\u305f\u3089\u3059. (3) \u86cb\u767d\u8cea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u30d7\u30ed\u30bb\u30b9\u306f\u30bf\u30a4\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u3092\u77ed\u7e2e\u3057\uff0c\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306e\u8ca0\u62c5\u3092\u8efd\u6e1b\u3057\u3066\u30d1\u30ef\u30fc\u30b9\u30c8\u30ed\u30fc\u30af\u306e\u5b8c\u4e86\u3092\u53ef\u80fd\u306b\u3059\u308b\uff0e\u3053\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u304b\u3089\uff0c\u30bf\u30a4\u30c1\u30f3\u306f\u67b6\u6a4b\u5f62\u6210\u306a\u3057\u3067\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u306a\u3044\u3060\u3051\u3067\u306a\u304f\uff0c\u30d1\u30ef\u30fc\u30b9\u30c8\u30ed\u30fc\u30af\u306e\u5b8c\u4e86\u3082\u307e\u305f\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u4f9d\u5b58\u3059\u308b\u3053\u3068\u3082\u660e\u3089\u304b\u3067\u3042\u308b\uff0e\u5f93\u3063\u3066\uff0c\u30bf\u30a4\u30df\u30f3\u3068\u30df\u30aa\u30b7\u30f3\u306f\u3001\u9ad8\u3044\u8ca0\u8377\u306b\u5bfe\u3057\u3066\u30b5\u30eb\u30b3\u30e1\u30a2\u3092\u77ed\u7e2e\u3059\u308b\u305f\u3081\u306b\u624b\u3092\u643a\u3048\u3066\u50cd\u304f\uff0e<\/figcaption><\/figure>\n

\u3000\u7b4b\u8089\u53ce\u7e2e\u6642\u306b\u3053\u308c\u304c\u767a\u751f\u3059\u308b\u969b\u306e\u3082\u3063\u3068\u3082\u3089\u3057\u3044\u30b7\u30ca\u30ea\u30aa\u306f\uff0c\u5404\u30bf\u30a4\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u304c 12 pN \u306e\u529b\u3092\u652f\u3048\u308b\u305f\u3081\u306b\uff0c\u3044\u3064\u30b5\u30eb\u30b3\u30e1\u30a2\u304c\u53d7\u52d5\u7684\u306b\u8ca0\u8377\u3055\u308c\u308b\u304b\u3067\u3042\u308b (86<\/a>,87<\/a>). \u7b4b\u8089\u306e\u6d3b\u52d5\u524d\u5f8c\u306b\u304a\u3044\u3066\u30bf\u30a4\u30c1\u30f3\u3068\u30df\u30aa\u30b7\u30f3\u9593\u3067\uff0c\u3069\u3046\u3084\u3063\u3066\u3053\u306e\u8ca0\u8377\u3092\u5206\u914d\u3057\u3066\u3044\u308b\u306e\u3060\u308d\u3046\uff1f\u4eee\u306b 147 \u672c\u306e\u53cc\u982d\u306e\u30df\u30aa\u30b7\u30f3\u5206\u5b50\u304c\u534a\u5206\u306e\u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u5185\u306b\u3042\u308a\uff0c\u307e\u305f\u30b5\u30eb\u30b3\u30e1\u30a2\u534a\u5206\u3042\u305f\u308a 6 \u500b\u306e\u30bf\u30a4\u30c1\u30f3\u5206\u5b50\u304c\u3042\u308b\u3068\u8003\u3048\u308b\u306a\u3089\uff0c\u30bf\u30a4\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u3042\u305f\u308a\u306e\u30df\u30aa\u30b7\u30f3\u5206\u5b50\u306e\u7d76\u5bfe\u7684\u306a\u6700\u5927\u6bd4\u306f 25 \u3067\u3042\u308b\uff0e\u3057\u304b\u3057\uff0c\u7b4b\u77ed\u7e2e\u4e2d\u306b\u3059\u3079\u3066\u306e\u30df\u30aa\u30b7\u30f3\u982d\u90e8\u304c\u30a2\u30af\u30c1\u30f3\u7d50\u5408\u90e8\u4f4d\u306b\u7d50\u5408\u3057\u3066\u3044\u308b\u308f\u3051\u3067\u306f\u306a\u3044\uff0e\u7b4b\u30df\u30aa\u30b7\u30f3\u306f\u975e\u9023\u7d9a\u30e2\u30fc\u30bf\u30fc\u3067\u3042\u308a\uff0c\u53ce\u7e2e\u4e2d\u306b\u30df\u30aa\u30b7\u30f3\u982d\u90e8\u304c\u30a2\u30af\u30c1\u30f3\u306b\u7d50\u5408\u3059\u308b\u30d1\u30fc\u30bb\u30f3\u30c6\u30fc\u30b8\u306f\u7d50\u5408\u90e8\u4f4d\u306b\u7d50\u5408\u53ef\u80fd\u306a\u6700\u5927\u6570\u306e 10-20 % \u7a0b\u5ea6\u3068\u63a8\u5b9a\u3055\u308c\u3066\u304d\u305f (88<\/a>). \u53ce\u7e2e\u4e2d\u306e\u7b4b\u7dda\u7dad\u306e\u8ffd\u5f93\u3092\u8a08\u6e2c\u3059\u308b\u3053\u3068\u3067\uff0c1 \u672c\u306e\u592a\u3044\u7dda\u7dad\u3042\u305f\u308a 84 \u500b (~ 24 %) \u307e\u3067\u306e\u30df\u30aa\u30b7\u30f3\u982d\u90e8\u304c\u5f93\u4e8b\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u3068\u8a08\u7b97\u3055\u308c\uff0c\u7d50\u5408\u3057\u305f\u982d\u90e8\u306e\u6570\u304c\u6025\u901f\u306b\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306e\u30aa\u30fc\u30d0\u30fc\u30e9\u30c3\u30d7\u304a\u3088\u3073\u77ed\u7e2e\u901f\u5ea6\u306e\u4e21\u8005\u3092\u5909\u5316\u3055\u305b\u3066\u3044\u305f (89<\/a>). \u3057\u304b\u3057\uff0c\u30bf\u30a4\u30c1\u30f3\u306e\u5f3e\u529b\u6027\u306f\u3053\u306e\u5b9f\u9a13\u3067\u306f\u8003\u616e\u3055\u308c\u3066\u304a\u3089\u305a\uff0c\u305d\u306e\u30c9\u30e1\u30a4\u30f3\u304c\u6298\u308a\u305f\u305f\u307e\u308c\u3066\u30bf\u30a4\u30c1\u30f3\u306f\u786c\u5316\u3057\u3066\u3044\u308b\u3068\u3057\u3066\uff0c\u53ce\u7e2e\u3057\u3066\u3044\u308b\u7b4b\u8089\u3067\u5f93\u4e8b\u3057\u3066\u3044\u308b\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306e\u6570\u3092\u904e\u5270\u63a8\u5b9a\u3059\u308b\u7d50\u679c\u3068\u306a\u3063\u305f\uff0e\u30a6\u30b5\u30ae\u306e\u5927\u8170\u7b4b\u306e 3.1 µm \u3084\u30d2\u30c8\u4f38\u5c55\u7b4b\u306e 3.4 µm \u306a\u3069\u751f\u7406\u7684\u306a\u30b5\u30eb\u30b3\u30e1\u30a2\u306e\u4f38\u5c55\u3067\u306f\uff0c\u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u3068\u7d30\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306e\u30aa\u30fc\u30d0\u30fc\u30e9\u30c3\u30d7\u306e\u7a0b\u5ea6\u304c\u6e1b\u5c11\u3059\u308b\u3068\u30df\u30aa\u30b7\u30f3\u982d\u90e8\u306e\u7d50\u5408\u6570\u304c\u5c11\u306a\u304f\u306a\u308b (10<\/a>,90<\/a>). \u3086\u3048\u306b\uff0c\u305d\u306e\u3088\u3046\u306a\u4f38\u5c55\u3055\u308c\u305f\u9577\u3055\u3067\u306f\uff0c\u53ce\u7e2e\u3092\u652f\u3048\u308b\u30bf\u30a4\u30c1\u30f3\u5206\u5b50 1 \u500b\u3042\u305f\u308a\u306e\u30df\u30aa\u30b7\u30f3\u982d\u90e8\u306e\u6570\u306f\u305f\u3063\u305f 1-2 \u500b\u306b\u904e\u304e\u306a\u3044\u3068\u8a08\u7b97\u3055\u308c\u305f\uff0e<\/p>\n

\u3000\u4eee\u8aac\u306e\u30b7\u30ca\u30ea\u30aa\u306b\u623b\u308b\u304c\uff0c\u30bf\u30a4\u30c1\u30f3\u306f\u7b4b\u53ce\u7e2e\u306b\u5148\u7acb\u3063\u3066 12 pN \u306e\u8ca0\u8377\u5168\u4f53\u3092\u652f\u3048\uff0c\u8fd1\u4f4d Ig \u30c9\u30e1\u30a4\u30f3\u3092\u52d5\u54e1\u3057\u3066\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c9\u72b6\u614b\u306b\u3059\u308b (Figure 3B<\/strong>, \u4e0a\u90e8\u30d1\u30cd\u30eb). \u4f8b\u3048\u3070\u91ce\u7403\u306e\u6295\u624b\u306e\u30ef\u30a4\u30f3\u30c9\u30a2\u30c3\u30d7\u4e2d\u306e\u3088\u3046\u306b\uff0c\u3053\u308c\u306f\u7b4b\u8089\u306e\u7206\u767a\u7684\u53ce\u7e2e\u306b\u5148\u7acb\u3063\u3066\u8d77\u304d\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\uff0e\u7b4b\u8089\u306e\u6d3b\u52d5\u5f8c\u306e\u6570\u30df\u30ea\u79d2\u9593\u3067\uff0c\u6700\u5927\u6570\u306e\u67b6\u6a4b\u304c\u5f62\u6210\u3055\u308c\u3066\u30b5\u30eb\u30b3\u30e1\u30a2\u5185\u306e\u4e09\u3064\u306e\u7b4b\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u304b\u3089\u306a\u308b\u6a5f\u68b0\u7684\u30cd\u30c3\u30c8\u30ef\u30fc\u30af\u306b\u8ca0\u8377\u304c\u5206\u914d\u3055\u308c\u308b\uff0e\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306f\u6700\u5927 6 pN \u306e\u52d5\u529b\u3092\u767a\u751f\u3055\u305b\u308b\u304c\uff0c\u30b5\u30eb\u30b3\u30e1\u30a2\u3092\u77ed\u7e2e\u3055\u305b\u308b\u307b\u3069\u5341\u5206\u3067\u306f\u306a\u304f\uff0c\u5168\u3066\u306e\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u306b\u304b\u304b\u308b\u8ca0\u8377\u3092 6 pN \u306b\u6e1b\u5f31\u3055\u305b\u308b (Figure 3B<\/strong>, \u4e2d\u90e8\u30d1\u30cd\u30eb). \u3053\u306e\u7d50\u679c\u307e\u305a\u975e\u5e38\u306b\u6025\u901f\u306b\u5168\u3066\u306e\u4f38\u5c55\u3055\u308c\u305f\u30bf\u30a4\u30c1\u30f3\u5206\u5b50\u304c\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u53cd\u52d5\u3092\u8d77\u3053\u3057\uff0c\u7d9a\u3044\u3066\u30bf\u30a4\u30c1\u30f3\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304c\u8d77\u304d\u3066\u6700\u5927\u53ef\u80fd\u306a\u4ed5\u4e8b\u3092\u306a\u3057\u3066\u529b\u3092\u56de\u5fa9\u3055\u305b\u308b (Figure 3A<\/strong>, \u9752\u66f2\u7dda\u30d4\u30fc\u30af). \u5f93\u6765\u306e\u4eee\u8aac\u3067\u306f\u30bf\u30a4\u30c1\u30f3\u306f\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u53cd\u52d5\u7d4c\u7531\u3067\u306e\u307f\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u56de\u5fa9\u3059\u308b\u3068\u307f\u306a\u3055\u308c\u3066\u3044\u305f\u304c\uff0c\u305d\u3046\u3067\u306f\u306a\u304f\uff0c\u3053\u3053\u3067\u56de\u5fa9\u3059\u308b\u30a8\u30cd\u30eb\u30ae\u30fc\u306e\u5927\u534a\u306f\u5b9f\u969b\u306b\u306f\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ce\u7e2e\u306b\u7531\u6765\u3057\u3066\u3044\u308b\u306e\u3067\u3042\u308b. 12 pN \u306e\u529b\u306b\u304a\u3044\u3066\uff0c\u6298\u308a\u305f\u305f\u307e\u308c\u305f\u72b6\u614b\u304b\u3089\u30e9\u30f3\u30c0\u30e0\u30b3\u30a4\u30eb\u306b\u884c\u304f\u3053\u3068\u3067\u9054\u6210\u3055\u308c\u308b\u8f2a\u90ed\u9577\u306e\u5909\u5316\u306f 15.8 nm \u3067\u3042\u308b. 6 pN \u307e\u3067\u306e\u529b\u3092\u6e1b\u5f31\u3055\u305b\u308b\u306b\u304a\u3044\u3066, 5.9 nm \u306e\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u53cd\u52d5\u306b\u5f15\u304d\u7d9a\u3044\u3066 9.9 nm \u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ce\u7e2e\u304c\u8d77\u304d\u308b (Figure 3B<\/strong>, \u4e2d\u90e8\u30d1\u30cd\u30eb). \u86cb\u767d\u8cea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306f\uff0c\u4f38\u5c55\u3055\u308c\u305f\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u304b\u3089\u306e\u56de\u5fa9\u306b\u3088\u308b\u30a8\u30cd\u30eb\u30ae\u30fc\u306e 63 % \u3092\u542b\u3080\u4e00\u65b9\uff0c\u5f3e\u6027\u53ce\u7e2e\u306f 37 % \u3092\u63d0\u4f9b\u3059\u308b\u306b\u904e\u304e\u306a\u3044\uff0e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ce\u7e2e\u3053\u305d\uff0c\u751f\u7406\u5b66\u7684\u306a\u529b\u306e\u7bc4\u56f2\u3067\u306e\u30bf\u30a4\u30c1\u30f3\u306e\u5f3e\u6027\u304a\u3088\u3073\u30a8\u30cd\u30eb\u30ae\u30fc\u84c4\u7a4d\u306e\u4e3b\u8981\u306a\u8981\u7d20\u306b\u4ed6\u306a\u3089\u306a\u3044<\/strong>\uff0e\u3086\u3048\u306b\uff0c\u3053\u306e\u30b7\u30ca\u30ea\u30aa\u3067\u306f, 12 pN \u4ee5\u4e0b\u306e\u8ca0\u8377\u3067\u306f\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3057\u305f Ig \u30c9\u30e1\u30a4\u30f3\u304c\u5931\u901f\u3057\u305f\u30e2\u30fc\u30bf\u30fc\u3092\u89e3\u653e\u3057\uff0c\u30b5\u30eb\u30b3\u30e1\u30a2\u306b\u306f\u4f38\u9577\u3059\u308b\u3088\u308a\u3080\u3057\u308d\u53ce\u7e2e\u3059\u308b\u3053\u3068\u3092\u8a31\u3059 (Figure 3B<\/strong>, \u4e0b\u90e8\u30d1\u30cd\u30eb). 12 pN \u3067\u30bf\u30a4\u30c1\u30f3\u3092\u4f38\u5c55\u3055\u305b\u308b\u3053\u3068\u3067\u84c4\u7a4d\u3055\u308c\u308b\u30a8\u30cd\u30eb\u30ae\u30fc\u306f\uff0c\u30e2\u30fc\u30bf\u30fc\u304c\u5f93\u4e8b\u3059\u308b\u6642\u306b\u306e\u307f\uff0c\u623b\u3063\u3066\u304f\u308b\uff0e\u3053\u306e\u7d20\u6674\u3089\u3057\u3044\u30e1\u30ab\u30cb\u30ba\u30e0\u306f, II \u578b\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u304c\u5931\u901f\u3057\u305f\u529b\u3092\u8d85\u3048\u308b\u8ca0\u8377\u3067\u3082\uff0c\u30b5\u30eb\u30b3\u30e1\u30a2\u304c\u52d5\u529b\u3092\u63d0\u4f9b\u3059\u308b\u53ef\u80fd\u6027\u3092\u5bb9\u6613\u306b\u62e1\u5f35\u3059\u308b\u3082\u306e\u3067\u3042\u308b\uff0e<\/p>\n

Titin folding dictates the optimal sarcomere length of a contracting muscle<\/h3>\n

\u3000\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ce\u7e2e\u306e\u5b58\u5728\u306f\uff0c\u7b4b\u751f\u7406\u5b66\u306e\u6559\u7fa9\u306e\u518d\u8a2a\u3092\u4fdd\u8a3c\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\uff0e\u4f8b\u3048\u3070\uff0c\u6b21\u306e\u3053\u3068\u306f\u4e00\u822c\u306b\u53d7\u3051\u5165\u308c\u3089\u308c\u3066\u3044\u308b\uff0e\u3064\u307e\u308a\uff0c\u4e0b\u80a2\u306e active force \u3068\u30b5\u30eb\u30b3\u30e1\u30a2\u9577\u3068\u306e\u95a2\u4fc2\u306f\u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u3068\u7d30\u3044\u30d5\u30a9\u30e9\u30e1\u30f3\u30c8\u306e\u30aa\u30fc\u30d0\u30fc\u30e9\u30c3\u30d7\u306e\u6e1b\u5c11\u306b\u3088\u308b\uff0c\u3068\u3044\u3046\u3053\u3068\u3067\u3042\u308b\uff0e\u3057\u304b\u3057\uff0c\u30d2\u30c8\u9aa8\u683c\u7b4b\u306b\u304a\u3044\u3066\u30b5\u30eb\u30b3\u30e1\u30a2\u9577 1.7-2.6 µm \u3067\u4e0a\u80a2\u306b\u304a\u3051\u308b\u529b\u306f\u65e2\u306b\u6700\u5927\u306b\u8fd1\u3044 (91<\/a>). \u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306f\u305b\u3044\u305c\u3044 1.6 µm \u307e\u3067\u306e\u9577\u3055\u3067\u3042\u308a\uff0c\u305d\u306e\u305f\u3081\u4e0a\u80a2\u306e\u3053\u306e\u90e8\u4f4d\u306b\u304a\u3044\u3066\u6e1b\u5c11\u3057\u305f\u529b\u306f\u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306e Z \u76e4\u306b\u5bfe\u3059\u308b\u5727\u7e2e\u3067\u306f\u306a\u3055\u305d\u3046\u3067\u3042\u308b\uff0e\u5225\u306e\u8aac\u660e\u3068\u3057\u3066\u53ef\u80fd\u6027\u304c\u3042\u308b\u306e\u306f\uff0c\u3053\u306e\u90e8\u4f4d\u3067\u306e\u7b49\u5c3a\u6027\u306e\u529b\u304c\u6e1b\u5c11\u3059\u308b\u306e\u306f\u5168\u3066\u306e\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u304c\u6298\u308a\u305f\u305f\u307e\u308c\u305f\u307e\u307e\u3067\u3042\u308b\u304b\u3089\uff0c\u3068\u3044\u3046\u3082\u306e\u304c\u3042\u308b\uff0e\u3086\u3048\u306b\uff0c\u30bf\u30a4\u30c1\u30f3\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u306e\u758e\u6c34\u6027\u5d29\u58ca\u306b\u3088\u308a\u767a\u751f\u3059\u308b\u4ed8\u52a0\u7684\u306a\u53ce\u7e2e\u529b\u3068\u3044\u3046\u3082\u306e\u306f\u5b58\u5728\u3057\u306a\u3044\uff0e\u4eee\u8aac\u7684\u306a\u9aa8\u683c\u7b4b\u3092\u8003\u3048\u3066\u307f\u3088\u3046\uff0e\u30bf\u30a4\u30c1\u30f3\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u304c\u4e2d\u9593\u7684\u306a\u9577\u3055\u3068\u786c\u5ea6\u3067\u5b58\u5728\u3059\u308b\u3068\u3059\u308b (Figure 4A<\/strong>, \u9752\u66f2\u7dda). \u3053\u306e\u30bf\u30a4\u30c1\u30f3\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u306f\u7b4b\u8089\u304b\u3089\u306e\u6700\u9ad8\u306e\u30d1\u30d5\u30a9\u30fc\u30de\u30f3\u30b9\u3092\u53ef\u80fd\u3068\u3059\u308b\uff0e\u3068\u3044\u3046\u306e\u306f\uff0c\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u78ba\u7387\u306b\u304a\u3051\u308b\u6700\u5927\u306e\u5909\u5316\u306f\uff0c\u6700\u5927\u306e active force \u767a\u751f\u3059\u308b\u90e8\u4f4d\u5185\u3067\u307e\u3063\u3059\u3050\u306b\u843d\u3061\u8fbc\u3080\u304b\u3089\u3067\u3042\u308b (Figure 4A, \u30d4\u30f3\u30af\u9818\u57df). \u30b0\u30ec\u30fc\u9818\u57df\u3067\u306e\u6700\u5927\u7b49\u5c3a\u529b\u306e\u8a08\u6e2c\u3067\u306f\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u95a2\u4e0e\u3092\u6b20\u3044\u3066\u3044\u308b\uff0e\u3068\u3044\u3046\u306e\u306f\uff0c\u8a72\u5f53\u3059\u308b\u30b5\u30eb\u30b3\u30e1\u30a2\u9577\u3067\u306e\u30bf\u30a4\u30c1\u30f3\u306f\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u3082\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u3082\u3044\u305a\u308c\u306e\u72b6\u614b\u3082\u3042\u308a\u3046\u308b\u304b\u3089\u3067\u3042\u308b (Figure 4A<\/strong>, \u30b0\u30ec\u30fc\u9818\u57df). \u3053\u308c\u3089\u306e\u4e00\u898b\u5358\u7d14\u306a\u5b9f\u9a13\u306f\u7b4b\u751f\u7406\u5b66\u306e\u57fa\u790e\u3092\u652f\u6301\u3057\u3066\u304a\u308a\uff0c\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u6587\u8108\u3067\u8003\u616e\u3059\u308b\u3068\uff0c\u305d\u3046\u7c21\u5358\u306a\u3082\u306e\u3067\u306f\u306a\u3044\uff0e<\/p>\n

\"\"<\/a>
Figure 4<\/strong>
\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306f\u751f\u7406\u5b66\u7684\u30b5\u30eb\u30b3\u30e1\u30a2\u9577\u3067\u751f\u3058\u308b<\/strong>
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Why protein folding?<\/h3>\n

\u3000\u7b4b\u8089\u306e\u53ef\u9006\u7684\u5909\u5f62\u306f\uff0c\u304a\u305d\u3089\u304f\u4ed6\u306e\u7d44\u7e54\u3068\u306f\u6bd4\u985e\u306e\u306a\u3044\u6750\u6599\u7279\u6027\u3067\u3042\u308b\uff0e\u7b4b\u8089\u306f\u6750\u6599\u75b2\u52b4\u3084\u30d2\u30b9\u30c6\u30ea\u30b7\u30b9\u306a\u3057\u306b\u4f38\u5c55\u3068\u5f1b\u7de9\u306e\u6301\u7d9a\u7684\u306a\u30b5\u30a4\u30af\u30eb\u3092\u7d4c\u9a13\u3057\u306a\u304f\u3066\u306f\u306a\u3089\u306a\u3044\uff0e\u3044\u304f\u3064\u304b\u306e\u7b4b\u8089\u7fa4\u3067\u306f\uff0c\u5b8c\u5168\u53ce\u7e2e\u304b\u3089\u5b8c\u5168\u4f38\u5c55\u72b6\u614b\u306b\u81f3\u308b\u3068\u30b5\u30eb\u30b3\u30e1\u30a2\u306e\u9577\u3055\u304c 300 nm \u306b\u307e\u3067\u4f38\u5c55\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\uff0c\u3053\u308c\u306f\u30b5\u30eb\u30b3\u30e1\u30a2\u9577\u306e 10 % \u306e\u5897\u52a0\u306b\u3042\u305f\u308b (90<\/a>,92<\/a>,93<\/a>). \u53ef\u9006\u7684\u306a\u7b4b\u5f3e\u6027\u306b\u5bc4\u4e0e\u3059\u308b\u305d\u306e\u86cb\u767d\u8cea\u306f\uff0c\u76f8\u53cd\u3059\u308b\u4e8c\u3064\u306e\u5f79\u5272\u3092\u6e80\u305f\u3055\u306a\u304f\u3066\u306f\u306a\u3089\u306a\u3044\uff0e\u3064\u307e\u308a\uff0c\u7b4b\u8089\u306f\u5bb9\u6613\u306b\u5909\u5f62\u3067\u304d\uff0c\u62ee\u6297\u7b4b\u306e\u53ce\u7e2e\u3092\u59a8\u5bb3\u3057\u306a\u3044\u7a0b\u5ea6\u306b\u306f\u67d4\u8edf\u3067\u306a\u304f\u3066\u306f\u306a\u3089\u306a\u3044\u304c\uff0c\u7b4b\u8089\u904e\u4f38\u5c55\u3084\u7b4b\u53ce\u7e2e\u6642\u306b\u89e3\u653e\u3055\u308c\u308b\u81a8\u5927\u306a\u5f3e\u6027\u30a8\u30cd\u30eb\u30ae\u30fc\u84c4\u7a4d\u306b\u3088\u308b\u640d\u50b7\u3092\u907f\u3051\u308b\u7a0b\u5ea6\u306b\u306f\u56fa\u304f\u306a\u304f\u3066\u306f\u306a\u3089\u306a\u3044\uff0e\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306f\uff0c\u6b63\u3057\u304f\u69cb\u9020\u5316\u3055\u308c\u308c\u3070\uff0c\u5f3e\u6027\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u84c4\u7a4d\u3057\u89e3\u653e\u3059\u308b\u305f\u3081\u306e\u512a\u308c\u305f\u6750\u6599\u3067\u3042\u308b\u3053\u3068\u304c\u5206\u304b\u3063\u305f\uff0e<\/p>\n

\u3000\u591a\u304f\u306e\u7d30\u80de\u9aa8\u683c\u69cb\u9020\u306f\u7b4b\u8089\u304c\u52d5\u4f5c\u3059\u308b\u7bc4\u56f2\u306e\u529b\u3067\u306f\u786c\u3044\uff0e\u5fae\u5c0f\u7ba1\u306f\u30df\u30ea\u30e1\u30fc\u30c8\u30eb\u4ee5\u4e0a\u306e\u6c38\u7d9a\u6027\u306e\u9577\u3055\u3092\u6709\u3057\u3066\u3044\u308b\u4e00\u65b9\u3067, f-\u30a2\u30af\u30c1\u30f3\u306f 10 \u30df\u30af\u30ed\u30f3\u306e\u6c38\u7d9a\u6027\u306e\u9577\u3055\u3092\u6709\u3057\u3066\u3044\u308b\u306e\u307f\u3067\u3042\u308b (94<\/a>). \u30b1\u30e9\u30c1\u30f3\u3084\u30d3\u30e1\u30f3\u30c1\u30f3\u306e\u3088\u3046\u306a\u4e2d\u9593\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306f\u6570\u767e\u30ca\u30ce\u30e1\u30fc\u30c8\u30eb\u304b\u3089\u30de\u30a4\u30af\u30ed\u30e1\u30fc\u30c8\u30eb\u307e\u3067\u306e\u6c38\u7d9a\u6027\u306e\u9577\u3055\u3092\u6709\u3059\u308b (95<\/a>). I \u578b\u30b3\u30e9\u30fc\u30b2\u30f3\u306f\u4e09\u3064\u306e\u72ec\u7acb\u3057\u305f\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u9396\u306e\u4e09\u91cd\u3089\u305b\u3093\u3067\u69cb\u6210\u3055\u308c\u308b\u304c\uff0c\u6570\u5343\u30ca\u30ce\u30e1\u30fc\u30c8\u30eb\u3082\u306e\u6c38\u7d9a\u6027\u306e\u9577\u3055\u3092\u6709\u3059\u308b\u3068\u8003\u3048\u3089\u308c\u3066\u3044\u308b (96<\/a>). \u3053\u308c\u3089\u306e\u7d20\u6750\u306e\u3046\u3061\u3067\u6709\u7528\u306a\u5f3e\u529b\u6027\u3092\u84c4\u7a4d\u3059\u308b\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u6709\u3059\u308b\u3082\u306e\u306f\u5b58\u5728\u3057\u306a\u3044\uff0e\u7406\u7531\u306f\u5358\u7d14\u3067\uff0c\u305d\u308c\u3089\u306f\u786c\u3059\u304e\u308b\u306e\u3067\u3042\u308b\uff0e\u6b63\u5e38\u306a\u7b4b\u8089\u306e\u52d5\u4f5c\u6642\u306b\u5358\u4e00\u306e\u86cb\u767d\u8cea\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u304c\u7d4c\u9a13\u3059\u308b\u529b\u306e\u7bc4\u56f2 (1-15 pN) \u3092\u8d85\u3048\u308b\u3068\uff0c\u3053\u308c\u3089\u306e\u86cb\u767d\u8cea\u306f\u65e2\u306b\u5b8c\u5168\u306b\u4f38\u5c55\u3055\u308c\u3066\u3044\u308b\u304c\uff0c\u786c\u3044\u7aff\u306e\u3088\u3046\u306b\uff0c\u7b4b\u8089\u306e\u4f38\u3073\u306b\u62b5\u6297\u3059\u308b\u3060\u308d\u3046\uff0e\u4ee3\u308f\u308a\u306b\uff0c\u975e\u69cb\u9020\u7684\u307e\u305f\u306f\u4f38\u5c55\u3057\u305f\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u9396\u306f\u591a\u69d8\u306a\u6280\u8853\u3067\u7279\u5fb4\u3065\u3051\u3089\u308c\u3066\u304d\u305f\u3057, Kuhn \u9577\u306e\u7bc4\u56f2\u306f 0.8 nm \u304b\u3089 1.2 mn \u3067\u3042\u308b\u3068\u793a\u3055\u308c\u305f\uff08\u6c38\u7d9a\u6027\u306e\u9577\u3055\u306f 0.4 nm \u304b\u3089 0.6 nm \u306b\u7b49\u3057\u3044\uff09. 5 pN \u4ee5\u4e0b\u306e\u529b\u3067\u306f\uff0c\u3042\u308b\u30e9\u30f3\u30c0\u30e0\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u30b3\u30a4\u30eb\u306f\u307b\u307c Hookean \u3070\u306d\u3068\u3057\u3066\u3075\u308b\u307e\u3046\u3060\u308d\u3046\u304c\uff0c\u3088\u308a\u5927\u304d\u306a\u529b\u3067\u306f\uff0c\u305d\u306e\u30e9\u30f3\u30c0\u30e0\u30b3\u30a4\u30eb\u306f\u975e\u7dda\u5f62\u306e\u3075\u308b\u307e\u3044\u3092\u793a\u3057\uff0c\u4f38\u5c55\u304c\u5897\u52a0\u3059\u308b\u306b\u3064\u308c\u3066\u3088\u308a\u6025\u901f\u306b\u529b\u3092\u5897\u3059\uff0e\u6700\u8fd1\u306e\u767a\u898b\u3067\u306f\uff0c\u751f\u7406\u7684\u306a\u7b4b\u8089\u306e\u6d3b\u52d5\u6642\u306e\u30bf\u30a4\u30c1\u30f3\u306f 4-15 pN \u306e\u529b\u306e\u4e0b\u3067\u306f\u6b21\u306e\u7591\u554f\u3092\u63d0\u793a\u3059\u308b\uff0e\u3053\u306e\u529b\u306e\u7bc4\u56f2\u3067\uff0c\u5358\u7d14\u306a\u30e9\u30f3\u30c0\u30e0\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u30b3\u30a4\u30eb\u3088\u308a\u3082\u591a\u304f\u306e\u5f3e\u6027\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u84c4\u7a4d\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3088\u3046\u306a\u5f62\u614b\u3092\uff0c\u3042\u308b\u7d20\u6750\u306b\u53d6\u3089\u305b\u308b\u3053\u3068\u306f\u53ef\u80fd\u306a\u306e\u3060\u308d\u3046\u304b\uff1f<\/p>\n

\u3000\u30bf\u30a4\u30c1\u30f3\u306e\u69cb\u9020\u306f\u3053\u306e\u554f\u984c\u306b\u5bfe\u3057\u3066\uff0c\u975e\u69cb\u9020\u30bb\u30b0\u30e1\u30f3\u30c8 (PEVK, N2B) \u304a\u3088\u3073\u591a\u304f\u306e\u30bf\u30f3\u30c7\u30e0\u304b\u3089\u306a\u308b Ig \u30c9\u30e1\u30a4\u30f3\u306e\u4e21\u8005\u3067\u3082\u3063\u3066\u7279\u5225\u306a\u56de\u7b54\u3092\u63d0\u4f9b\u3057\u3066\u304f\u308c\u308b. Ig \u30c9\u30e1\u30a4\u30f3\u3078\u5206\u7bc0\u5316\u3057\u305f\u30bf\u30a4\u30c1\u30f3 I-band \u306f\u7b4b\u8089\u306e\u5f3e\u529b\u6027\u3092\u751f\u7406\u7684\u306a\u529b\u306e\u7bc4\u56f2\u3067\u5927\u304d\u304f\u5897\u52a0\u3055\u305b\u308b\uff0e\u305d\u306e\u30dd\u30ea\u30da\u30d7\u30c1\u30c9\u306f\u81a8\u5927\u306a\u30a8\u30cd\u30eb\u30ae\u30fc (W = FΔL) \u3092\u84c4\u7a4d\u3059\u308b\uff0e\u3053\u3053\u3067 F \u306f FJC \u306b\u3088\u308a\u5b9a\u7fa9\u3055\u308c\u308b\u529b\u3067\u3042\u308a L \u306f\u89b3\u6e2c\u3055\u308c\u308b\u9577\u3055\u3067\u3042\u308b\uff0e\u4eee\u306b\u30bf\u30a4\u30c1\u30f3\u304c\u975e\u69cb\u9020\u30da\u30d7\u30c1\u30c9\u306e\u307f\u542b\u6709\u3059\u308b\u3068\u4eee\u5b9a\u3059\u308b\u3068, 12 pN \u304b\u3089 6 pN \u3078\u306e\u529b\u306e\u6e1b\u5f31\u306f\u305d\u306e\u4f38\u5c55\u9577\u306e 63 % \u306e\u5d29\u58ca\u306b\u7531\u6765\u3059\u308b\u3053\u3068\u306b\u306a\u308b. 90 \u500b\u307e\u3067\u306e\u30a2\u30df\u30ce\u9178\u3092\u542b\u6709\u3059\u308b\u975e\u69cb\u9020\u30bb\u30b0\u30e1\u30f3\u30c8\u306b\u3068\u3063\u3066\uff0c\u305d\u306e\u9577\u3055\u304c 15.7 nm \u304b\u3089 9.9 nm \u306b\u7e2e\u9000\u3059\u308b\u3053\u3068\u306b\u306a\u308a\uff0c\u305d\u306e\u7d50\u679c\u89e3\u653e\u3055\u308c\u308b\u5f3e\u6027\u30a8\u30cd\u30eb\u30ae\u30fc\u306f 34.8 zJ \u3068\u306a\u308b\uff0e\u4eca\u5ea6\u306f\u540c\u3058 90 \u500b\u306e\u30a2\u30df\u30ce\u9178\u3067\u5206\u7bc0\u5316\u3057\u3066\u6298\u308a\u305f\u305f\u3080\u3053\u3068\u306e\u3067\u304d\u308b\u3082\u306e\u3092\u8003\u3048\u3066\u307f\u3088\u3046\uff0e12 \u304b\u3089 6 pN \u306b\u529b\u304c\u6e1b\u5f31\u3059\u308b\u3068\uff0c\u307e\u305a 34.8 zJ\u3000\u304c\u653e\u51fa\u3055\u308c\uff0c\u6b21\u306b\u4ed8\u52a0\u7684\u306a 59.2 zJ \u304c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u4f34\u3063\u3066\u653e\u51fa\u3055\u308c\u308b\uff0e\u30a8\u30cd\u30eb\u30ae\u30fc\u56de\u5fa9\u306e\u5408\u8a08\u306f\u4eca\u3084 94 zJ \u306b\u5897\u52a0\u3057, 3 \u5206\u306e 2 \u306f\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ce\u7e2e\u306b\u7531\u6765\u3059\u308b\u3053\u3068\u306b\u306a\u308b. Ig \u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3068\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306f\u30bf\u30a4\u30c1\u30f3\u306b\u30c0\u30a4\u30ca\u30df\u30c3\u30af\u306a\u9069\u5fdc\u3092\u8a31\u3057\u3066\u30b5\u30eb\u30b3\u30e1\u30a2\u9577\u306e\u5e45\u5e83\u3044\u7bc4\u56f2\u306b\u308f\u305f\u308b\u5f35\u529b\u3092\u63d0\u4f9b\u3059\u308b\u3060\u3051\u3067\u306f\u306a\u304f\uff0c\u7b4b\u8089\u5185\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u84c4\u7a4d\u3068\u89e3\u653e\u3092\u5897\u3084\u3059\u3053\u3068\u306b\u3082\u306a\u308b\uff0e\u30a8\u30cd\u30eb\u30ae\u30fc\u84c4\u7a4d\u3068\u89e3\u653e\u306f\uff0c\u975e\u5e38\u306b\u7cbe\u5bc6\u306a\u529b\u306e\u7bc4\u56f2\u5185\u3067\u306e\u307f\u53ef\u80fd\u3067\u3042\u308a\uff0c\u305d\u3053\u3067\u306f\u86cb\u767d\u8cea\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3068\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304c\u8d77\u304d\u3066\u3044\u308b\uff0e\u6211\u3005\u306f\uff0c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u78ba\u7387\u3092\u307e\u305f\u3044\u3067\uff0c\u30bf\u30a4\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306e\u5f3e\u6027\u30dd\u30c6\u30f3\u30b7\u30e3\u30eb\u306e\u84c4\u7a4d\u3092\u6d3b\u7528\u3059\u308b\u3088\u3046\u306a\u9577\u3055\u3068\u529b\u3067\u7b4b\u8089\u304c\u52d5\u4f5c\u3059\u308b\u3088\u3046\u8abf\u6574\u3055\u308c\u3066\u3044\u308b\u3068\u63d0\u6848\u3059\u308b\uff0e\u3055\u3089\u306b\uff0c\u7d30\u80de\u30b7\u30b0\u30ca\u30ea\u30f3\u30b0\u3092\u901a\u3058\u3066\u30bf\u30a4\u30c1\u30f3\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3092\u64cd\u4f5c\u3059\u308b\u3053\u3068\u3067\uff0c\u7b4b\u8089\u306f\u5f3e\u6027\u30a8\u30cd\u30eb\u30ae\u30fc\u84c4\u7a4d\u3092\u5909\u66f4\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\uff0e<\/p>\n

The titin folding reaction can be biochemically tuned<\/h3>\n

\u3000\u30bf\u30a4\u30c1\u30f3\u306f\u7b4b\u8089\u306e\u7d14\u7c8b\u306a\u6a5f\u68b0\u7684\u86cb\u767d\u8cea\u3067\u3082\u306a\u3051\u308c\u3070\u69cb\u9020\u86cb\u767d\u8cea\u3067\u3082\u306a\u3044\uff0e\u305d\u308c\u306f\u7b4b\u8089\u306b\u304a\u3051\u308b\u5316\u5b66\u7684\u304b\u3064\u7269\u7406\u7684\u30b9\u30c8\u30ec\u30b9\u306e\u7a4d\u5206\u5668\u3067\u3082\u3042\u308a\uff0c\u30b7\u30b0\u30ca\u30eb\u7d4c\u8def\u306e\u4e00\u90e8\u3067\u3042\u308a\uff0c\u6700\u7d42\u7684\u306b\u306f\u907a\u4f1d\u5b50\u767a\u73fe\u306b\u5f71\u97ff\u3059\u308b\uff0e\u30bf\u30a4\u30c1\u30f3\u306e\u5f3e\u6027\u30bb\u30b0\u30e1\u30f3\u30c8\u306f\u7ffb\u8a33\u5f8c\u4fee\u98fe\u306e\u4e3b\u8981\u306a\u6a19\u7684\u3067\u3042\u308b\u3068\u793a\u3055\u308c\u305f\uff0e\u30bf\u30a4\u30c1\u30f3\u306e\u975e\u69cb\u9020\u90e8\u4f4d\u5185\u306e\u6b8b\u57fa\u306e\u30ea\u30f3\u9178\u5316\u306f\u30bf\u30a4\u30c1\u30f3\u306e\u5f3e\u6027\u306b\u304a\u3044\u3066\u306f\u7a4f\u3084\u304b\u306a\u5909\u5316\u3067\u3042\u308b\u3068\u793a\u3055\u308c\u305f\u4e00\u65b9\u3067 (97<\/a>,98<\/a>,99<\/a>), \u3055\u3089\u306b\u5287\u7684\u306a\u5909\u5316\u306f I-band \u306e Ig \u30c9\u30e1\u30a4\u30f3\u306e\u6a19\u7684\u5316\u306b\u3088\u308a\u9054\u6210\u3055\u308c\u308b\uff0e\u30de\u30a6\u30b9\u5fc3\u7b4b\u6897\u585e\u30e2\u30c7\u30eb\u306b\u304a\u3044\u3066\u306f\u865a\u8840\u518d\u704c\u6d41\u969c\u5bb3\u306b\u3088\u308b\u9178\u5316\u30b9\u30c8\u30ec\u30b9\u306f\u30b5\u30eb\u30b3\u30e1\u30a2\u86cb\u767d\u8cea\u306e S-\u30b0\u30eb\u30bf\u30c1\u30aa\u30cb\u30eb\u5316\u3092\u62db\u304d\uff0c\u305d\u308c\u306b\u306f\u975e\u5e38\u306b\u5de8\u5927\u306a\u5206\u5b50\u91cf\u306e\u86cb\u767d\u8cea\u304c\u542b\u307e\u308c\uff0c\u30bf\u30a4\u30c1\u30f3\u3067\u3042\u308b\u3068\u4e3b\u5f35\u3055\u308c\u3066\u3044\u308b (100<\/a>). \u30bf\u30a4\u30c1\u30f3 I-band \u306e Ig \u30c9\u30e1\u30a4\u30f3\u306f\u4e0d\u91e3\u308a\u5408\u3044\u306b\u9ad8\u3044\u30b7\u30b9\u30c6\u30a4\u30f3\u6b8b\u57fa\u3092\u542b\u6709\u3057\u3066\u3044\u308b\uff0e\u3057\u304b\u3057\uff0c\u3053\u308c\u3089\u5927\u534a\u306e\u30b7\u30b9\u30c6\u30a4\u30f3\u5074\u9396\u306f\u8b0e\u3081\u3044\u3066\u3044\u308b\uff0e\u305d\u308c\u3089\u306f Ig \u6298\u308a\u7573\u307f\u306e\u758e\u6c34\u6027\u30b3\u30a2\u306b\u57cb\u3081\u8fbc\u307e\u308c\u3066\u304a\u308a\uff0c\u6eb6\u5a92\u306b\u30a2\u30af\u30bb\u30b9\u3067\u304d\u306a\u3044\u3053\u3068\u3092\u610f\u5473\u3057\u3066\u3044\u308b\uff0e\u305d\u308c\u3089\u306f Ig \u30c9\u30e1\u30a4\u30f3\u304c\u4f38\u5c55\u3057\u59cb\u3081\u306a\u3044\u9650\u308a, S-\u30b0\u30eb\u30bf\u30c1\u30aa\u30cb\u30eb\u5316\u3067\u304d\u306a\u3044. Alegre-Cebollada \u3068\u305d\u306e\u4ef2\u9593\u304c\u793a\u3057\u305f\u3068\u3053\u308d\u3067\u306f\uff0c\u5358\u5206\u5b50 force-clamp AFM \u3092\u7528\u3044\u3066\uff0c\u5358\u5206\u5b50\u30bf\u30a4\u30c1\u30f3 I91 \u30c9\u30e1\u30a4\u30f3\u306e S-\u30b0\u30eb\u30bf\u30c1\u30aa\u30cb\u30eb\u5316\u304c\u529b\u306b\u3088\u308a\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3057\u305f (78<\/a>). \u3042\u308b\u91cd\u5927\u306a\u767a\u898b\u304c\u3042\u3063\u305f. S-\u30b0\u30eb\u30bf\u30c1\u30aa\u30cb\u30eb\u5316\u304c I91 \u30c9\u30e1\u30a4\u30f3\u306e\u758e\u6c34\u6027\u30b3\u30a2\u5185\u90e8\u306b\u597d\u307e\u3057\u304f\u306a\u3044\u7acb\u4f53\u69cb\u9020\u3092\u4f5c\u308a\u51fa\u3057\uff0c\u305d\u308c\u304c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3092\u963b\u5bb3\u3057\u305f\u306e\u3067\u3042\u308b\uff0e\u3086\u3048\u306b\u305d\u308c\u306f\u9178\u5316\u578b\u30b0\u30eb\u30bf\u30c1\u30aa\u30f3\u306f\u5358\u4e00\u5fc3\u7b4b\u5185\u306e\u53d7\u52d5\u7684\u5f35\u529b\u3092\u5927\u5e45\u306b\u6e1b\u5c11\u3055\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3068\u8a3c\u660e\u3059\u308b\u3053\u3068\u306b\u306a\u3063\u305f\u304c\uff0c\u5fc3\u7b4b\u7d30\u80de\u304c\u4f38\u5c55\u3057\u3066 Ig \u30c9\u30e1\u30a4\u30f3\u3092\u52d5\u54e1\u3057\uff0c\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u72b6\u614b\u306b\u306a\u3063\u305f\u5f8c\u306b\u9650\u3089\u308c\u308b\uff0e\u30ea\u30f3\u9178\u5316\u306e\u3088\u3046\u306b, S-\u30b0\u30eb\u30bf\u30c1\u30aa\u30cb\u30eb\u5316\u306f\u53ef\u9006\u7684\u3067\u3042\u308a\uff0c\u7b4b\u7d44\u7e54\u5185\u90e8\u3067\u9175\u7d20\u7684\u306a\u5207\u65ad\u3084\u5909\u6027\u3092\u901a\u3058\u3066\u30b0\u30eb\u30bf\u30c1\u30aa\u30f3\u3092\u6e1b\u5c11\u3055\u305b\u308b\u3053\u3068\u3055\u3048\u3059\u308b\uff0e\u3057\u304b\u3057\uff0c\u30ea\u30f3\u9178\u5316\u306f\u30bf\u30a4\u30c1\u30f3\u5206\u5b50\u306e\u786c\u5ea6\u307e\u305f\u306f Kuhn \u9577\u306b\u304a\u3044\u3066\u5c0f\u3055\u306a\u5909\u5316\u3057\u304b\u3082\u305f\u3089\u3055\u306a\u3044\u4e00\u65b9\u3067, Ig \u6298\u308a\u7573\u307f\u306e S-\u30b0\u30eb\u30bf\u30c1\u30aa\u30cb\u30eb\u5316\u3084\u4ed6\u306e\u975e\u7279\u7570\u7684\u9178\u5316\u306f\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53cd\u5fdc\u306b\u5927\u898f\u6a21\u306a\u9589\u585e\u3092\u3082\u305f\u3089\u3059\u3053\u3068\u304c\u3067\u304d\u308b\uff0e\u4eee\u306b\u5168\u30bf\u30a4\u30c1\u30f3\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304c\u963b\u5bb3\u3055\u308c\u305f\u3089\uff0c\u305d\u306e\u5f3e\u529b\u7279\u6027\u306f Figure 4B<\/strong> \u306b\u793a\u3057\u305f\u9752\u306e\u70b9\u7dda\u306e\u3088\u3046\u306b\u3075\u308b\u307e\u3046\u3060\u308d\u3046\uff0e\u53d7\u52d5\u7684\u5f35\u529b\u306e\u55aa\u5931\u306f\u4ed6\u306e\u30b5\u30eb\u30b3\u30e1\u30a2\u86cb\u767d\u8cea\u306b\u3088\u308a\u611f\u77e5\u3055\u308c\u308b\u3068\u3053\u308d\u3068\u306a\u308a\uff0c\u7d30\u80de\u30b7\u30b0\u30ca\u30ea\u30f3\u30b0\u306b\u5909\u63db\u3055\u308c\u308b\u7d50\u679c\u3068\u306a\u308b\u3068\u4e88\u60f3\u3055\u308c\u308b\uff0e\u4ed6\u306e\u4e3b\u8981\u306a\u30bf\u30a4\u30c1\u30f3\u306e\u9178\u5316\u7684\u4fee\u98fe\u306f\uff0c\u5206\u5b50\u5185\u306e\u30b8\u30b9\u30eb\u30d5\u30a3\u30c9\u7d50\u5408\u3067\u3042\u308b\uff0e\u305d\u306e\u30b8\u30b9\u30eb\u30d5\u30a3\u30c9\u7d50\u5408\u306f\uff0c\u6a5f\u68b0\u7684\u5b89\u5b9a\u6027\u3084\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u7387\u3078\u306e\u5927\u5e45\u306a\u5f71\u97ff\u306a\u3057\u306b, Ig \u30c9\u30e1\u30a4\u30f3\u304c\u6298\u308a\u305f\u305f\u307e\u308c\u308b\u7b87\u6240\u3067\u306e\uff0c\u5927\u5e45\u306a\u529b\u306e\u5897\u52a0\u3092\u53ef\u80fd\u3068\u3059\u308b (80<\/a>). \u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u78ba\u7387\u30b7\u30d5\u30c8\u306e\u7d50\u679c\uff0c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53cd\u5fdc\u304c\u53ce\u7e2e\u6642\u306b\u3088\u308a\u9ad8\u3044\u529b\u3092\u767a\u751f\u3055\u305b\u308b\u7d50\u679c\u3068\u306a\u308b (Figure 4B<\/strong>, \u91d1\u8272\u66f2\u7dda). \u9178\u5316\u30b9\u30c8\u30ec\u30b9\u306b\u52a0\u3048\u3066\uff0c\u30a2\u30b7\u30c9\u30fc\u30b7\u30b9\u304c\u7b4b\u8089\u306e\u6052\u5e38\u6027\u306b\u5bfe\u3059\u308b\u4e00\u822c\u7684\u306a\u6442\u52d5\u3067\u3042\u308a\uff0c\u86cb\u767d\u8cea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u76f4\u63a5\u7684\u306a\u7d50\u679c\u3092\u3082\u305f\u3089\u3059 (101<\/a>). pH 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\u65b0\u305f\u306a\u8996\u70b9\u306f\u6b21\u306e\u3088\u3046\u306a\u3082\u306e\u3067\u3082\u3042\u308b\uff0e\u3064\u307e\u308a\uff0c\u7b4b\u7279\u7570\u7684\u30b7\u30e3\u30da\u30ed\u30f3\u306f\u7269\u7406\u7684\u4f38\u5c55\u306b\u5bfe\u3057\u3066\u30bf\u30a4\u30c1\u30f3\u306e I-band \u30bb\u30b0\u30e1\u30f3\u30c8\u306b\u52d5\u54e1\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a (103<\/a>), \u3042\u308b\u7a2e\u306e\u7b4b\u75be\u60a3\u306b\u304a\u3044\u3066\u30ec\u30d9\u30eb\u304c\u3072\u3069\u304f\u5909\u66f4\u3055\u308c\u308b\u5206\u5b50\u30b7\u30e3\u30da\u30ed\u30f3\u304c\u3042\u308b (104<\/a>) \u3068\u3044\u3046\u3053\u3068\u3067\u3042\u308b\uff0e\u3053\u308c\u3089\u306e\u30b7\u30e3\u30da\u30ed\u30f3\u306e\u3044\u304f\u3064\u304b\uff0c\u4f8b\u3048\u3070 αB-\u30af\u30ea\u30b9\u30bf\u30ea\u30f3\u306f\uff0c\u6298\u308a\u305f\u305f\u307e\u308c\u305f Ig \u30c9\u30e1\u30a4\u30f3\u306b\u76f4\u63a5\u7d50\u5408\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3042\u308a\uff0c\u305d\u308c\u3089\u3092\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u5bfe\u3057\u3066\u3088\u308a\u62b5\u6297\u6027\u306b\u3059\u308b (Figure 4B<\/strong>, \u8d64\u66f2\u7dda) (103<\/a>,105<\/a>). \u5358\u5206\u5b50\u539f\u5b50\u9593\u529b\u5206\u5149\u6cd5\u306f\u6700\u8fd1\u5206\u5b50\u30b7\u30e3\u30da\u30ed\u30f3\u306e\u53ef\u80fd\u6027\u3092\u660e\u3089\u304b\u306b\u3057\u59cb\u3081\u3066\u304a\u308a\uff0c\u51dd\u96c6\u3092\u9632\u3050\u3060\u3051\u3067\u306a\u304f\uff0c\u3042\u308b\u7a2e\u306e\u86cb\u767d\u8cea\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53cd\u5fdc\u3092\u52a0\u901f\u3057\u3066\u3044\u308b\u3068\u3055\u308c\u308b (Haldar, \u672a\u516c\u958b\u30c7\u30fc\u30bf). \u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u529b\u5b66\u306b\u304a\u3051\u308b\u5897\u52a0\u306f\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53cd\u5fdc\u304b\u3089\u306e\u51fa\u529b\u5897\u52a0\u3068\u540c\u7fa9\u3067\u3042\u308a\uff0c\u3086\u3048\u306b 3 \u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u30e2\u30c7\u30eb\u306b\u5f93\u3063\u3066\u7b4b\u53ce\u7e2e\u306b\u76f4\u63a5\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u3060\u308d\u3046\uff0e<\/p>\n

A rechargeable gravitational battery<\/h3>\n

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\u306b\u3082\u9054\u3059\u308b\u3068\u63a8\u5b9a\u3055\u308c\u3066\u3044\u308b\uff0e\u305d\u306e\u529b\u306e\u95a2\u4fc2\u306f\u7570\u306a\u308b\u30c9\u30e1\u30a4\u30f3\u306b\u3088\u308b\u3082\u306e\u3067\u3042\u308d\u3046\u304c\uff0c\u751f\u4f53\u5185\u3067\u306e\u529b\u306e\u653e\u51fa\u306f\u3088\u308a\u8907\u96d1\u3067\u3042\u308b\u306b\u9055\u3044\u306a\u3044\uff0e\u52d5\u7269\u306e\u904b\u52d5\u306b\u304a\u3044\u3066\u7b4b\u8089\u304c\u3069\u3046\u3084\u3063\u3066\u52d5\u4f5c\u3059\u308b\u304b\u3092\u6975\u7aef\u306b\u5358\u7d14\u5316\u3059\u308b\u3068\uff0c\u30d2\u30e9\u30e1\u7b4b\u306e\u3088\u3046\u306a\u811a\u306e\u7b4b\u8089\u306f\u305d\u306e\u9759\u6b62\u72b6\u614b\u304b\u3089\u4f38\u5c55\u4f4d\u7f6e\u306b\u79fb\u52d5\u3057\uff0c\u9ad8\u3044\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u30bf\u30a4\u30c1\u30f3\u306b\u5909\u63db\u3059\u308b (Figure 5A<\/strong>, \u4e0b\u90e8\u30d1\u30cd\u30eb 1 \u304b\u3089 2). \u5f8c\u306b\uff0c\u5206\u5b50\u30e2\u30fc\u30bf\u30fc\u3068 ATP \u52a0\u6c34\u5206\u89e3\u304c\u53c2\u52a0\u3057\u305f\u5f8c\u306b\u30bf\u30a4\u30c1\u30f3\u306b\u5909\u63db\u3055\u308c\u308b\u529b\u306f\u6e1b\u5c11\u3057\uff0c\u30bf\u30a4\u30c1\u30f3\u304b\u3089\u6a5f\u68b0\u7684\u4ed5\u4e8b\u304c\u653e\u51fa\u3055\u308c\u3066\u7b4b\u8089\u304c\u53ce\u7e2e\u3059\u308b (Figure 5A<\/strong>, \u4e0b\u90e8\u30d1\u30cd\u30eb 2 \u304b\u3089 3). \u30bf\u30a4\u30c1\u30f3\u30c9\u30e1\u30a4\u30f3\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u30fb\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u4e0a\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u5909\u63db\u306e\u610f\u5473\u304b\u3089\u306f\uff0c\u4e0a\u8a18\u3067\u63d0\u6848\u3057\u305f\u84c4\u7a4d\u30fb\u653e\u51fa\u30b5\u30a4\u30af\u30eb\u306f\uff0c\u30bf\u30a4\u30c1\u30f3\u5206\u5b50\u306e\u81ea\u7531\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u80cc\u666f\u306b\u3057\u305f\u878d\u5408\u30d7\u30ed\u30bb\u30b9\u3068\u3057\u3066\u63cf\u304f\u3053\u3068\u304c\u3067\u304d\u308b (Figure 5B<\/strong>). 12 pN \u3067\u306e\uff08\u7dd1\u8272\u80cc\u666f\uff09\u84c4\u7a4d\u30d5\u30a7\u30fc\u30ba\u4e2d\u305d\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u80cc\u666f\u306f\u4f38\u5c55\u72b6\u614b\u306b\u50be\u3044\u3066\u304a\u308a\uff08\u5927\u4f38\u5c55\uff09\uff0c\u305d\u306e\u305f\u3081\u30bf\u30a4\u30c1\u30f3\u30c9\u30e1\u30a4\u30f3\u304c\u4f38\u5c55\u3057\uff0c\u9806\u6b21\u81ea\u7531\u30a8\u30cd\u30eb\u30ae\u30fc\u969c\u58c1\u3092\u8d8a\u3048\u308b\u306e\u3092\u9858\u3063\u3066\u3044\u308b\uff0e\u5916\u529b\u304c 6 pN \u306b\u307e\u3067\u6e1b\u5c11\u3059\u308b\u3068\uff08\u8d64\u8272\u80cc\u666f\uff09\uff0c\u305d\u306e\u5206\u5b50\u306f\u518d\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u3088\u308a\u77ed\u7e2e\u3057\uff0c\u305d\u306e\u30c9\u30e1\u30a4\u30f3\u306f\u53cd\u5bfe\u65b9\u5411\u306b\u969c\u58c1\u3092\u9858\u3044\uff0c\u3086\u3048\u306b\u53ce\u7e2e\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u653e\u51fa\u3059\u308b\uff0e<\/p>\n

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Figure 5. \u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u30e1\u30ab\u30cb\u30ba\u30e0\u3068\u3057\u3066\u306e\u7b4b\u53ce\u7e2e\u30e2\u30c7\u30eb<\/strong>
(A)<\/strong> \u7b4b\u53ce\u7e2e\u306b\u304a\u3051\u308b\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u30fb\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u5f79\u5272\uff08\u30d1\u30cd\u30eb\u4e0a\u90e8\uff09\uff0e \u30bf\u30a4\u30c1\u30f3\u30c9\u30e1\u30a4\u30f3\u306f\u300c\u30d0\u30c3\u30c6\u30ea\u30fc\u300d\u3068\u3057\u3066\u6a5f\u80fd\u3057\uff0c\u53ce\u7e2e\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u3088\u308a\u84c4\u3048\uff0c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u77ed\u7e2e\u6642\u306b\u4f9b\u7d66\u3059\u308b\uff0e\u7b4b\u8089\u304c\u4f38\u5c55\u3059\u308b\u6642\uff0c\u6a5f\u68b0\u7684\u5f35\u529b\u304c\u5897\u52a0\u3057\uff0c\u30bf\u30a4\u30c1\u30f3\u30c9\u30e1\u30a4\u30f3\u304c\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c9\u3059\u308b\uff08\u84c4\u7a4d\u30d5\u30a7\u30fc\u30ba (1)-(2)). \u7b4b\u8089\u304c\u53ce\u7e2e\u3059\u308b\u3068\u3001\u30df\u30aa\u30b7\u30f3\u982d\u90e8\u304c\u5f93\u4e8b\u3057\u3066\u30bf\u30a4\u30c1\u30f3\u306b\u304b\u304b\u308b\u529b\u304c 6 pN \u306b\u4f4e\u4e0b\u3057, Ig \u30c9\u30e1\u30a4\u30f3\u304c\u518d\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3057\u3066\u77ed\u7e2e\u3057\uff0c\u53ce\u7e2e\u30a8\u30cd\u30eb\u30ae\u30fc\u304c\u4f9b\u7d66\u3055\u308c\u3066\u7b4b\u53ce\u7e2e\u3092\u88dc\u52a9\u3059\u308b (3)\uff08\u4e0b\u90e8\u30d1\u30cd\u30eb\uff09\uff0e\u904b\u52d5\u4e2d\u306e\u52d5\u7269\u306e\u7b4b\u8089\u306e\u4f38\u5f35-\u53ce\u7e2e\u306e\u305f\u3081\u306e\u7c21\u7565\u5316\u3055\u308c\u305f\u30e2\u30c7\u30eb\uff0c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304a\u3088\u3073\u518d\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u6642\u306e\u4e09\u76f8\u306e\u30bf\u30a4\u30c1\u30f3\u3092\u793a\u3059\uff0e(B)<\/strong> \u30bf\u30a4\u30c1\u30f3\u88dc\u52a9\u306e\u7b4b\u53ce\u7e2e\u306e\u81ea\u7531\u30a8\u30cd\u30eb\u30ae\u30fc\u666f\u89b3\u89e3\u91c8\uff0e\u4f38\u5c55\u30d5\u30a7\u30fc\u30ba\u306b\u304a\u3044\u3066\u306f\u305d\u306e\u666f\u89b3\u306f\u5897\u52a0\u3059\u308b\u529b\u306b\u3088\u308a\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c9\u72b6\u614b\uff08\u3088\u308a\u9577\u3044\u9577\u3055\uff09\u306b\u50be\u304d (12 pN, \u9752\u8272), \u30bf\u30a4\u30c1\u30f3\u306f\u53ce\u7e2e\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u305d\u306e\u30c9\u30e1\u30a4\u30f3\u306e\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306b\u3088\u308a\u84c4\u7a4d\u3059\u308b\uff0e\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u304c\u53c2\u52a0\u3059\u308b\u6642\uff0c\u30bf\u30a4\u30c1\u30f3\u306b\u304b\u304b\u308b\u8ca0\u8377\u306f 6 pN \u306b\u6e1b\u5c11\u3059\u308b\u305f\u3081\uff0c\u305d\u306e\u666f\u89b3\u306f\u6298\u308a\u305f\u305f\u307e\u308c\u305f\u72b6\u614b\u306b\u6709\u5229\u3068\u306a\u308a\uff0c\u81ea\u7531\u30a8\u30cd\u30eb\u30ae\u30fc\u969c\u58c1\u3092\u4e57\u308a\u8d8a\u3048\u3066\u30c9\u30e1\u30a4\u30f3\u3092\u6298\u308a\u305f\u305f\u3080\u3053\u3068\u304c\u53ef\u80fd\u3068\u306a\u308a\uff0c\u5206\u5b50\u5168\u9577\u3092\u77ed\u7e2e\u3057\u3066\u53ce\u7e2e\u306e\u4ed5\u4e8b\u3092\u63d0\u4f9b\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3068\u306a\u308b\uff0e(C)<\/strong>\u3053\u306e\u4eee\u8aac\u3092\u8a66\u9a13\u3059\u308b\u306b\u306f\uff0c\u907a\u4f1d\u7684\u306b\u30b3\u30fc\u30c9\u5316\u3055\u308c\u305f\u529b\u5b66\u30bb\u30f3\u30b5\u30fc\u3092\u30bf\u30a4\u30c1\u30f3\u914d\u5217\u306b\u7d44\u307f\u8fbc\u3080\u3053\u3068\u304c\u5fc5\u8981\u3067\u3042\u308b\uff0e\u3053\u306e\u3088\u3046\u306a\u8a2d\u8a08\u306e\u4e00\u3064\u306f\uff0c\u30a8\u30ad\u30bd\u30f3 I86 \u3068 I87 \u3068\u306e\u9593\u306b\u5c0e\u5165\u3055\u308c\u305f HaloTag \u304a\u3088\u3073 SNAP \u30bf\u30b0\u5171\u6709\u6a19\u8b58\u6280\u8853\u3092\u5229\u7528\u3057\u3066\u63d0\u4f9b\u3055\u308c\u308b\uff0e\u4eee\u8aac\u7684\u306a\u7dd1\u8272\u4f9b\u4e0e\u4f53\u86cd\u5149\u56e3\u304a\u3088\u3073\u8d64\u8272\u30a2\u30af\u30bb\u30d7\u30bf\u30fc\u86cd\u5149\u56e3\u3092\u7528\u3044\u3066\uff0c\u30d1\u30cd\u30eb (A) \u306b\u793a\u3055\u308c\u308b\u6b69\u307f\u3092\u901a\u3057\u3066\uff0c\u7b4b\u8089\u306e FRET \u6bd4\u306f\u6700\u5927\u304b\u3089\u6700\u5c0f\u306b\u5909\u5316\u3059\u308b\uff0e\u3053\u308c\u306f\u751f\u4f53\u5185\u3067\u306e\u30bf\u30a4\u30c1\u30f3 1 \u5206\u5b50\u3042\u305f\u308a\u306b\u304b\u304b\u308b\u529b\u3092\u76f4\u63a5\u8aad\u307f\u51fa\u3059\u3053\u3068\u3092\u53ef\u80fd\u3068\u3059\u308b\uff0e<\/figcaption><\/figure>\n

\u3000\u6211\u3005\u306e\u73fe\u5728\u306e\u89b3\u5bdf\u306e\u9650\u754c\u306f\u78c1\u6c17\u30d4\u30f3\u30bb\u30c3\u30c8\u3067\u63a2\u7d22\u3057\u305f 8 \u56de\u7e70\u308a\u8fd4\u3057 Ig \u30c9\u30e1\u30a4\u30f3\u306e 6 pN \u3067\u306e\u9045\u3044\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u7387\u3067\u3042\u308b (Figure 1B<\/strong>). 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\u6700\u5f8c\u306b\uff0c\u30b5\u30eb\u30b3\u30e1\u30a2\u306f\u30bf\u30a4\u30c1\u30f3\u7279\u7570\u7684\u30b7\u30e3\u30da\u30ed\u30f3\u3067\u6df7\u96d1\u3057\u305f\u74b0\u5883\u3067\u3042\u308a\uff0c\u305d\u308c\u304c\u6211\u3005\u306e\u6e2c\u5b9a\u3088\u308a\u3082\u3055\u3089\u306b\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u7387\u3092\u5897\u52a0\u3055\u305b\u3066\u3044\u308b\u306b\u9055\u3044\u306a\u3044 (102<\/a>,103<\/a>). \u3053\u308c\u3089\u306e\u52b9\u679c\u306e\u5168\u3066\u304c, Figure 1B<\/strong> \u306b\u793a\u3057\u305f\u5b9f\u9a13\u3067\u306f\u6b20\u3051\u3066\u3044\u308b\u304c\uff0c\u30b5\u30eb\u30b3\u30e1\u30a2\u5185\u90e8\u3067\u306e\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u51fa\u529b\u3092\u5f37\u5316\u3059\u308b\u3060\u308d\u3046\uff0e<\/p>\n

Synchronization of titin folding with actomyosin contraction<\/h3>\n

\u3000\u30bf\u30a4\u30c1\u30f3\u306e\u305f\u3081\u306e\u6211\u3005\u306e\u4eee\u8aac\u306f\u7b4b\u53ce\u7e2e\u72b6\u614b\u3092\u5f37\u5316\u3059\u308b\u3082\u306e\u3067\u3042\u308b\uff0e\u3064\u307e\u308a\uff0c\u30bf\u30a4\u30c1\u30f3\u3068\u30df\u30aa\u30b7\u30f3\u306f\u5354\u529b\u3057\u3066\u50cd\u304f\uff0e\u305d\u308c\u306f\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304c\u67b6\u6a4b\u306e\u53c2\u4e0e\u306b\u3088\u308a\u5f15\u304d\u8d77\u3053\u3055\u308c\u308b\u5916\u529b\u306e\u6e1b\u5c11\u306b\u4f9d\u5b58\u3057\u3066\u3044\u308b\u305f\u3081\u3067\u3042\u308b\uff0e\u3053\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u793a\u5506\u3059\u308b\u3068\u3053\u308d\u306f\uff0c\u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306b\u4ed8\u7740\u3057\u3066\u3044\u308b 6 \u3064\u306e\u30bf\u30a4\u30c1\u30f3\u5206\u5b50\u306f\u540c\u6642\u306b\u6298\u308a\u305f\u305f\u307e\u308c\u308b\u3060\u308d\u3046\u3068\u3044\u3046\u3053\u3068\u3067\u3042\u308b\uff0e\u5358\u5206\u5b50\u539f\u5b50\u9593\u529b\u5206\u5149\u6cd5\u306e\u8a66\u9a13\u306f\u5e73\u884c\u306a\u30dd\u30ea\u86cb\u767d\u8cea\u306e\u30b9\u30b1\u30fc\u30ea\u30f3\u30b0\u306e\u6cd5\u5247\u3092\u8a3c\u660e\u3057\u305f\uff0e\u86cb\u767d\u8cea\u30c9\u30e1\u30a4\u30f3\u304c\u5e73\u884c\u306b\u4f38\u5c55\u3055\u308c\u308b\u6642\uff0c\u305d\u308c\u3089\u306f\u540c\u6642\u306b\u7834\u88c2\u3057\uff0c\u529b\u306f\u4e8c\u500d\u3068\u306a\u308a\uff0c\u6c38\u7d9a\u9577\u306f\u534a\u5206\u3068\u306a\u308b (109<\/a>,110<\/a>). \u305d\u308c\u3086\u3048\uff0c\u6211\u3005\u306e\u793a\u5506\u3059\u308b\u3068\u3053\u308d\u306f\uff0c6 \u672c\u306e\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u3082\u540c\u6642\u306b\u6298\u308a\u305f\u305f\u307e\u308c\uff0c\u305d\u308c\u306f\u592a\u3044\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u5468\u56f2\u306e\u5e73\u884c\u306b\u914d\u5217\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u306b\u3088\u308b\uff0c\u3068\u3044\u3046\u3082\u306e\u3067\u3042\u308b. 6 \u672c\u306e\u5e73\u884c\u306a\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u306e\u6700\u521d\u306e\u4e00\u672c\u304c\u78ba\u7387\u7684\u306b\u6298\u308a\u305f\u305f\u307e\u308c\u308b\u6642\uff0c\u4ed6\u306e 5 \u672c\u306b\u304b\u304b\u308b\u529b\u306f\u5373\u5ea7\u306b\u6e1b\u5c11\u3059\u308b\uff0e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3084\u30a2\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u7387\u306f\u5916\u529b\u306b\u6307\u6570\u95a2\u6570\u7684\u306b\u4f9d\u5b58\u3057\uff0c\u5916\u529b\u306e\u307b\u3093\u306e\u5c11\u3057\u306e\u6e1b\u5c11\u3067\u3055\u3048\uff0c\u4ed6\u306e\u5e73\u884c\u306a\u30c9\u30e1\u30a4\u30f3\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3092\u52a0\u901f\u3059\u308b\u3060\u308d\u3046\uff0e\u3086\u3048\u306b\uff0c\u30bf\u30a4\u30c1\u30f3 Ig \u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304b\u3089\u306e\u51fa\u529b\u306f\u9ad8\u5ea6\u306b\u5354\u8abf\u7684\u3067\u3042\u308b\uff0e\u3055\u3089\u306b\uff0c\u30a2\u30af\u30c1\u30f3-\u30df\u30aa\u30b7\u30f3\u53ce\u7e2e\u304c\u540c\u671f\u3057\u3066\u3044\u308b\u306e\u306b\u30bf\u30a4\u30c1\u30f3\u306f\u305d\u3046\u3067\u306f\u306a\u3044\uff0c\u3068\u8a00\u3046\u306e\u306f\u8aa4\u89e3\u3092\u62db\u304f (111<\/a>). \u6b21\u306e\u70b9\u306f\u4e00\u822c\u306b\u53d7\u3051\u5165\u308c\u3089\u308c\u3066\u3044\u308b\uff0e\u3064\u307e\u308a\uff0c\u7b4b\u8089\u77ed\u7e2e\u306b\u99c6\u52d5\u3055\u308c\u308b\u30df\u30aa\u30b7\u30f3\u306f “\u30b5\u30a4\u30af\u30ea\u30c3\u30af\u975e\u540c\u671f ATP \u99c6\u52d5\u5f62\u30a2\u30af\u30c1\u30f3-\u30df\u30aa\u30b7\u30f3\u53ce\u7e2e” \u306b\u3088\u308a\u767a\u751f\u3059\u308b\u3068 (112). \u67b6\u6a4b\u5f62\u6210\u306f\u30df\u30aa\u30b7\u30f3\u982d\u90e8\u3068\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306e\u62e1\u6563\u906d\u9047\u306b\u4f9d\u5b58\u3057\u3066\u304a\u308a\uff0c\u78ba\u7387\u7684\u30d7\u30ed\u30bb\u30b9\u3067\u3042\u308b\u3068\u5b9a\u7fa9\u3055\u308c\u308b\uff0e\u52a0\u3048\u3066, ATP \u52a0\u6c34\u5206\u89e3\u306b\u3088\u308a\u653e\u51fa\u3055\u308c\u308b\u30a8\u30cd\u30eb\u30ae\u30fc\u306f kT \u3088\u308a\u5927\u304d\u306a\u30aa\u30fc\u30c0\u30fc\u306e\u5927\u304d\u3055\u306b\u904e\u304e\u306a\u3044\u305f\u3081\uff0c\u305d\u306e\u5f8c\u306e\u30df\u30aa\u30b7\u30f3\u306e\u69cb\u9020\u5909\u5316\u306f\uff0c\u5c11\u306a\u304f\u3068\u3082\u90e8\u5206\u7684\u306b\u306f\uff0c\u30d6\u30e9\u30a6\u30f3\u904b\u52d5\u306b\u4f9d\u5b58\u3059\u308b (113<\/a>). \u3053\u306e\u8996\u70b9\u304b\u3089\uff0c\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306e\u30b5\u30a4\u30af\u30ea\u30f3\u30b0\u3068\u30bf\u30a4\u30c1\u30f3 Ig \u30c9\u30e1\u30a4\u30f3\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3068\u306f\u5171\u306b\u78ba\u7387\u7684\u3067\u3042\u308b\u304c\uff0c\u6211\u3005\u306e\u30e2\u30c7\u30eb\u3067\u306f\uff0c\u7b4b\u8089\u306e\u5316\u5b66\u7684\u6d3b\u6027\u5316\u306b\u304a\u3044\u3066\u540c\u6642\u306b\u767a\u751f\u3059\u308b\uff0e<\/p>\n

Testable predictions and the techniques to examine them<\/h3>\n

\u3000\u7b4b\u8089\u306f\u8907\u96d1\u306a\u30b7\u30b9\u30c6\u30e0\u3067\u3042\u308a\uff0c\u968e\u5c64\u69cb\u9020\u306b\u914d\u5217\u3055\u308c\u305f\u86cb\u767d\u8cea\u306e\u3044\u304f\u3064\u304b\u306e\u76f8\u4e92\u4f5c\u7528\u3092\u542b\u3093\u3067\u3044\u308b\uff0e\u5206\u5b50\u30ec\u30d9\u30eb\u304b\u3089\u3053\u308c\u3089\u306e\u5404\u86cb\u767d\u8cea\u306e\u7279\u5b9a\u306e\u5f79\u5272\u3092\u5b9a\u7fa9\u3059\u308b\u3053\u3068\u306f\u56f0\u96e3\u306a\u4e8b\u696d\u3067\u3042\u308b\uff0e\u3057\u304b\u3057\uff0c\u30bf\u30a4\u30c1\u30f3\u5206\u5b50\u306e\u30bf\u30f3\u30c7\u30e0\u30e2\u30b8\u30e5\u30fc\u30eb\u5f0f\u914d\u7f6e\u306f\u7b4b\u8089\u306e\u53d7\u52d5\u7684\u5f35\u529b\u306e\u5f79\u5272\u306b\u5358\u306b\u5c02\u5ff5\u3059\u308b\u3060\u3051\u3067\u306f\u306a\u304f\uff0c\u5b9f\u969b\u306b\u3053\u308c\u3089\u306e\u30c9\u30e1\u30a4\u30f3\u304c\u5916\u529b\u306e\u7bc4\u56f2\u5185\u3067\u4f38\u5c55\u3057\u518d\u5ea6\u6298\u308a\u305f\u305f\u307e\u308c\u3066\u52d5\u4f5c\u3059\u308b\u3053\u3068\u306f\uff0c\u4eca\u3084\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u3088\u3046\u306b\u898b\u3048\u308b\uff0e\u7b4b\u8089\u306e\u77ed\u7e2e\u6642\u306b\u3042\u305f\u308a\uff0c\u3053\u308c\u306f\u53ce\u7e2e\u306e\u4ed5\u4e8b\u3092\u767a\u751f\u3055\u305b\u308b\u30bf\u30a4\u30c1\u30f3\u306e\u6d3b\u52d5\u6a5f\u80fd\u3092\u4f34\u3046\uff0e\u3057\u304b\u3057\uff0c\u7b4b\u53ce\u7e2e\u306b\u304a\u3051\u308b\u30bf\u30a4\u30c1\u30f3\u306e\u7cbe\u5bc6\u306a\u5f79\u5272\u304a\u3088\u3073\uff0c\u3044\u304b\u306b\u3057\u3066\u30c9\u30e1\u30a4\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u51fa\u529b\u304c\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306e\u51fa\u529b\u3068\u76f8\u4e92\u4f5c\u7528\u3059\u308b\u304b\u306e\u8a73\u7d30\u306a\u30d3\u30b8\u30e7\u30f3\u3092\u63d0\u4f9b\u3059\u308b\u305f\u3081\u306b\uff0c\u591a\u304f\u306e\u5b9f\u9a13\u306e\u4ed5\u4e8b\u304c\u6b8b\u3055\u308c\u3066\u3044\u308b\uff0e\u5168\u4f53\u3092\u901a\u3058\u3066\u8b70\u8ad6\u3055\u308c\u305f\u4eee\u8aac\u306f\u691c\u8a3c\u53ef\u80fd\u306a\u4e88\u6e2c\u306e\u9375\u3092\u4f5c\u308b\uff0e\u3064\u307e\u308a\uff0c\u7b4b\u8089\u306e\u6d3b\u6027\u5316\u306f\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306e\u53c2\u52a0\u3078\u3068\u7e4b\u304c\u308a\uff0c\u30bf\u30a4\u30c1\u30f3\u3078\u306e\u8ca0\u8377\u3092\u6e1b\u5c11\u3055\u305b\u3066\uff0c\u4ee5\u524d\u306b\u4f38\u5c55\u72b6\u614b\u306b\u52d5\u54e1\u3055\u308c\u305f Ig \u30c9\u30e1\u30a4\u30f3\u3092\u6298\u308a\u305f\u305f\u3080\u30c8\u30ea\u30ac\u30fc\u3068\u306a\u308b (10<\/a>). \u6211\u3005\u306f\u81ea\u5206\u81ea\u8eab\u306b\u554f\u308f\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\uff0e\u30bf\u30a4\u30c1\u30f3\u306e\u3053\u306e\u30a8\u30cd\u30eb\u30ae\u30c3\u30b7\u30e5\u306a\u8ca2\u732e\u306f\u3044\u304b\u306b\u3057\u3066\u5206\u96e2\u3057\u305f\u306e\u304b\uff1f\u307e\u305f\uff0c\u73fe\u5728\u306e\u751f\u7269\u7269\u7406\u5b66\u7684\u30c4\u30fc\u30eb\u306f\u5341\u5206\u306a\u306e\u304b\uff1f\u3068\uff0e<\/p>\n

\u3000\u539f\u5b50\u9593\u529b\u5206\u5149\u5b66\u8005\u305f\u3061\u306f\uff0c\u5168\u7d44\u7e54\u30ec\u30d9\u30eb\u3067\u306e\u5358\u5206\u5b50\u6280\u8853\u306e\u7cbe\u5ea6\u3092\u3082\u305f\u3089\u3057\u3066\u304f\u308c\u308b\u30c4\u30fc\u30eb\u3092\u6301\u3064\u3053\u3068\u3092\u5fc3\u304b\u3089\u671b\u3093\u3067\u3044\u308b\uff0e\u3042\u308b\u7a0b\u5ea6\uff0c\u3053\u308c\u306f\u4e00\u822c\u306b\u306f\u30b3\u30fc\u30c9\u5316\u3055\u308c\u305f\u529b\u5b66\u30bb\u30f3\u30b5\u30fc\u3068\u5171\u306b\u8a8d\u8b58\u3055\u308c\u3066\u304d\u3066\u304a\u308a\uff0c\u57f9\u990a\u7d30\u80de\u306e\u529b\u3092\u652f\u3048\u308b\u7d30\u80de\u9aa8\u683c\u6210\u5206\u5185\u90e8\u3067\u767a\u73fe\u3057\u3066\u3044\u308b (114<\/a>). \u3053\u308c\u3089\u306e\u529b\u5b66\u30bb\u30f3\u30b5\u30fc\u306f\u4e8c\u7a2e\u306e\u86cd\u5149\u86cb\u767d\u8cea\u3092\u7528\u3044\uff0c\u975e\u653e\u5c04\u6027\u79fb\u52d5\u3092\u901a\u3058\u3066\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u5909\u63db\u3059\u308b\u3053\u3068\u304c\u3067\u304d (FRET), \u77ed\u3044\u975e\u69cb\u9020\u5316\u307e\u305f\u306f\u69cb\u9020\u5316\u30da\u30d7\u30c1\u30c9\u306b\u5206\u96e2\u3057\u3066\u3044\u308b\uff0e\u3053\u308c\u3089\u306e\u4e00\u822c\u306b\u30b3\u30fc\u30c9\u5316\u3055\u308c\u305f\u529b\u5b66\u30bb\u30f3\u30b5\u30fc\u306f\u30b9\u30da\u30af\u30c8\u30ea\u30f3\u86cb\u767d (115<\/a>), \u30d3\u30f3\u30ad\u30e5\u30ea\u30f3\u304a\u3088\u3073\u30bf\u30ea\u30f3 (116<\/a>,117<\/a>), \u305d\u3057\u3066\u6838\u30cd\u30b9\u30d7\u30ea\u30f3 (118<\/a>) \u306b\u7d44\u307f\u8fbc\u307e\u308c\uff0c\u3053\u308c\u3089\u306f\u5168\u3066\u7d30\u80de\u5185\u30b9\u30c8\u30ec\u30b9\u7dda\u7dad\u304b\u3089\u306e\u5f35\u529b\u306e\u6750\u6599\u3067\u3042\u308b\uff0e\u529b\u5b66\u30bb\u30f3\u30b5\u30fc\u3092\u6709\u3059\u308b\u86cb\u767d\u8cea\u306b\u304b\u304b\u308b\u5f35\u529b\u304c\u5897\u52a0\u3059\u308b\u306b\u3064\u308c, FRET \u30c9\u30ca\u30fc\u304a\u3088\u3073\u30a2\u30af\u30bb\u30d7\u30bf\u30fc\u306f\u5206\u96e2\u3057\u3066 FRET \u7387\u304c\u4f4e\u4e0b\u3059\u308b\uff0e\u3053\u308c\u3089\u306e\u529b\u5b66\u30bb\u30f3\u30b5\u30fc\u306f FRET \u7387\u3092\u8a08\u6e2c\u3059\u308b\u3053\u3068\u3067\u30ad\u30e3\u30ea\u30d6\u30ec\u30fc\u30b7\u30e7\u30f3\u3055\u308c\uff0c\u305d\u308c\u306f 0-10 pN \u306e\u7bc4\u56f2\u3092\u8d85\u3048\u308b\u5358\u5206\u5b50\u539f\u5b50\u9593\u529b\u5206\u5149\u6cd5\u3092\u7528\u3044\u3066\u9069\u7528\u3055\u308c\u305f\u5916\u529b\u306e\u4e0b\u3067\u306e FRET \u7387\u3067\u3042\u308b (119<\/a>). \u30bf\u30a4\u30c1\u30f3 I-band \u306f\u6280\u8853\u8005\u306b\u3068\u3063\u3066 Figure 5<\/strong> \u306b\u63cf\u304b\u308c\u305f\u3088\u3046\u306b\u529b\u5b66\u30bb\u30f3\u30b5\u30fc\u306e\u7406\u60f3\u7684\u306a\u90e8\u4f4d\u306b\u306a\u308a\u3046\u308b\uff0e\u7d30\u80de\u57f9\u990a\u5b9f\u9a13\u3068\u7570\u306a\u308a\uff0c\u5236\u5fa1\u3055\u308c\u3066\u3044\u306a\u3044\u65b9\u6cd5\u3067\u7d30\u80de\u63a5\u7740\u5f8c\u306e\u7d30\u80de\u9aa8\u683c\u5f35\u529b\u304c\u5897\u52a0\u3059\u308b\u3068\u3053\u308d\u3067\u306f\uff0c\u30bf\u30a4\u30c1\u30f3\u306b\u304b\u304b\u308b\u305d\u306e\u5f35\u529b\u306f\u7b4b\u7dda\u7dad\u727d\u5f15\u88c5\u7f6e\u306b\u3088\u308a\u7d76\u5999\u306b\u8abf\u7bc0\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b (9<\/a>,108<\/a>,120<\/a>). \u30bf\u30a4\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306e\u30b5\u30eb\u30b3\u30e1\u30a2\u5185\u90e8\u3067\u306e\u7d71\u4e00\u3055\u308c\u305f\u914d\u7f6e\u306f\uff0c\u767a\u8272\u56e3\u304c\u9ad8\u5ea6\u306b\u6574\u5217\u3059\u308b\u3053\u3068\u3092\u78ba\u4fdd\u3057\u3066\u304a\u308a\uff0c\u7121\u50b7\u306e\u7b4b\u8089\u5185\u306e\u86cd\u5149\u8aad\u307f\u53d6\u308a\u306e\u512a\u308c\u305f\u30b7\u30b0\u30ca\u30eb\u30ce\u30a4\u30ba\u6bd4\u3092\u63d0\u4f9b\u3057\u3066\u304f\u308c\u308b\uff0e\u6700\u3082\u91cd\u8981\u306a\u3053\u3068\u3060\u304c\uff0c\u30bf\u30a4\u30c1\u30f3\u306b\u304b\u304b\u308b\u5916\u529b\u306f\u30d0\u30b9\u5185\u306e\u30ab\u30eb\u30b7\u30a6\u30e0\u6fc3\u5ea6\u4e0a\u6607\u306b\u3088\u308a\u8abf\u7bc0\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\uff0c\u305d\u306e\u7d50\u679c\u67b6\u6a4b\u5f62\u6210\u304c\u30bf\u30a4\u30c1\u30f3\u304b\u3089\u529b\u3092\u77ed\u7d61\u3055\u305b\u308b\u7d50\u679c\u3068\u306a\u308b\u3053\u3068\u3092\u6211\u3005\u306f\u793a\u5506\u3057\u305f\uff0e\u7b4b\u53ce\u7e2e\u306b\u304a\u3051\u308b\u6211\u3005\u306e 3 \u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u30e2\u30c7\u30eb\u306b\u5f93\u3046\u3068\uff0c\u751f\u7406\u7684\u30b9\u30c8\u30ec\u30c3\u30c1\u306e\u4e0b\u3067\u306e\u7b4b\u8089\u5185\u306e\u67b6\u6a4b\u5f62\u6210\u306f\u5404\u30bf\u30a4\u30c1\u30f3\u306b\u304b\u304b\u308b\u529b\u3092 10 pN \u4ee5\u4e0b\u306b\u6e1b\u5c11\u3055\u305b\uff0c\u305d\u3053\u3067\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u304c\u30d4\u30fc\u30af\u51fa\u529b\u3092\u653e\u51fa\u3059\u308b\uff0e\u4e00\u822c\u7684\u306b\u30b3\u30fc\u30c9\u5316\u3055\u308c\u305f\u529b\u5b66\u30bb\u30f3\u30b5\u30fc\u304c\u30bf\u30a4\u30c1\u30f3\u3054\u3068\u306e\u529b\u3092\u6c7a\u5b9a\u3059\u308b\u3053\u3068\u3092\u53ef\u80fd\u3068\u3059\u308b\u3060\u3051\u3067\u306f\u306a\u304f\uff0c\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ce\u7e2e\u3067\u306e\u30b5\u30eb\u30b3\u30e1\u30a2\u9577\u3082\u307e\u305f\u30c8\u30ea\u30ac\u30fc\u3055\u308c\u308b\uff0e\u5404\u7b4b\u7fa4\u306f\u7570\u306a\u308b\u9577\u3055\u306e\u30bf\u30a4\u30c1\u30f3\u30a2\u30a4\u30bd\u30d5\u30a9\u30fc\u30e0\u3092\u6709\u3057\u3066\u3044\u308b\uff0e\u4f8b\u3048\u3070\u7570\u306a\u308b 40 \u30bb\u30c3\u30c8\u306e\u30a6\u30b5\u30ae\u306e\u7b4b\u8089\u306e\u3088\u3046\u306b (87<\/a>). \u6211\u3005\u306e\u793a\u5506\u3059\u308b\u3068\u3053\u308d\u3067\u306f\uff0c\u3053\u306e\u9577\u3055\u306f\u6b21\u306e\u3088\u3046\u306b\u30d7\u30ed\u30b0\u30e9\u30e0\u3055\u308c\u3066\u3044\u308b\uff0e\u3064\u307e\u308a\uff0c\u30bf\u30a4\u30c1\u30f3\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ce\u7e2e\u306f\uff0c\u7279\u5b9a\u306e\u7b4b\u8089\u7fa4\u306e\u6700\u9069\u306a\u4ed5\u4e8b\u3092\u3059\u308b\u30b5\u30eb\u30b3\u30e1\u30a2\u9577\u306e\u4e2d\u3067\u767a\u751f\u3059\u308b\u3068\uff0e\u305d\u306e\u3088\u3046\u306a\u30c4\u30fc\u30eb\u306f\u7b4b\u8089\u306e\u52d5\u4f5c\u306e\u7814\u7a76\u306b\u3068\u3063\u3066\uff0c\u751f\u4f53\u5185\u3084\u8a66\u9a13\u7ba1\u5185\u3044\u305a\u308c\u306b\u304a\u3044\u3066\u3082\u975e\u5e38\u306b\u8cb4\u91cd\u3067\u3042\u308a\uff0c\u30bf\u30a4\u30c1\u30f3\u304a\u3088\u3073\u30bf\u30a4\u30c1\u30f3\u306b\u304b\u304b\u308b\u8ca0\u8377\u3092\u6e1b\u5c11\u3055\u305b\u308b\u306e\u306b\u5fc5\u8981\u306a\u67b6\u6a4b\u306e\u6570\u306b\u3088\u308a\u7d4c\u9a13\u3059\u308b\u751f\u7406\u5b66\u7684\u306a\u529b\u3092\u3088\u308a\u826f\u304f\u7406\u89e3\u3059\u308b\u305f\u3081\u306e\u5206\u91ce\u3078\u306e\u79fb\u52d5\u306e\u52a9\u3051\u3068\u306a\u308b\u3060\u308d\u3046\uff0e\u3053\u308c\u3089\u306e\u4e8c\u3064\u306e\u529b\u306f\uff0c\u30bf\u30a4\u30c1\u30f3\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u901f\u5ea6\u3068\u4f75\u305b\u3066\uff0c\u7b4b\u53ce\u7e2e\u6642\u306e\u30bf\u30a4\u30c1\u30f3\u3068\u30df\u30aa\u30b7\u30f3\u306e\u76f8\u5bfe\u7684\u306a\u51fa\u529b\u3092\u6c7a\u5b9a\u3059\u308b\u306e\u306b\u91cd\u8981\u3067\u3042\u308b\uff0e<\/p>\n

\u3000\u305d\u306e\u3088\u3046\u306a\u751f\u4f53\u5185\u30c4\u30fc\u30eb\u306e\u767a\u5c55\u3078\u306e\u6700\u521d\u306e\u6bb5\u968e\u306f\u9054\u6210\u3055\u308c\u3066\u3044\u308b. I-band \u9060\u4f4d\u90e8\u5206\u3078\u306e HaloTag \u9175\u7d20\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u3092\u6709\u3059\u308b\u30e2\u30c7\u30eb\u30de\u30a6\u30b9\u306f\u30bf\u30a4\u30c1\u30f3\u306e\u751f\u7269\u7269\u7406\u5b66\u7684\u7279\u6027\u306e\u305f\u3081\u306e\u591a\u76ee\u7684\u30c4\u30fc\u30eb\u3068\u3057\u3066\u5949\u4ed5\u3057\u305f (121<\/a>). HaloTag \u9175\u7d20\u306e\u4f7f\u7528\u306f\uff0c\u30ac\u30e9\u30b9\u88fd\u30ab\u30d0\u30fc\u30b9\u30ea\u30c3\u30d7\u306b\u5358\u5206\u5b50\u3092\u727d\u5f15\u3059\u308b\u5b9f\u9a13\u306e\u305f\u3081\u306e\u5171\u6709\u7d50\u5408\u30a2\u30f3\u30ab\u30fc\u86cb\u767d\u8cea\u3068\u3057\u3066\uff0c\u6211\u3005\u306e\u30e9\u30dc\u3067\u958b\u767a\u3055\u308c\u305f\u3082\u306e\u3067\u3042\u308a (41<\/a>,49<\/a>), \u86cd\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306e\u5e83\u7bc4\u56f2\u306e\u53d6\u5f97\u3092\u53ef\u80fd\u3068\u3057\u305f (122<\/a>,123<\/a>). HaloTag \u306f\u30af\u30ed\u30ed\u30a2\u30eb\u30ab\u30f3\u57fa\u8cea\u3092\u8a8d\u8b58\u3057\u3066\u5171\u6709\u7d50\u5408\u3057\uff0c\u69d8\u3005\u306a\u6709\u6a5f\u767a\u8272\u56e3\u3068\u4ed8\u7740\u3057\u3066\u512a\u308c\u305f\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u7279\u6027\u3092\u6301\u3064\uff0e\u6297\u4f53\u3068\u9055\u3063\u3066\uff0c\u30af\u30ed\u30ed\u30a2\u30eb\u30ab\u30f3\u30ea\u30ac\u30f3\u30c9\u306f\u975e\u5e38\u306b\u5c0f\u3055\u304f\uff0c\u5bb9\u6613\u306b\u30b5\u30eb\u30b3\u30e1\u30a2\u306b\u6d78\u900f\u3057\u3066\u9ad8\u6fc3\u5ea6\u306e\u6a19\u8b58\u306b\u9054\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b. SNAP-Tag, CLIP-Tag \u3084 TMP-tag (122) \u306a\u3069\u306e\u76f4\u4ea4\u6a19\u8b58\u90e8\u4f4d\u3092 HaloTag \u306b\u7d9a\u3051\u3066\u914d\u7f6e\u3059\u308b\u3053\u3068\u3067\uff0c\u4e0a\u8ff0\u3057\u305f\u30bb\u30f3\u30b5\u30fc\u306b\u8fd1\u4f3c\u3057\u305f\u4e00\u822c\u7684\u306b\u30b3\u30fc\u30c9\u5316\u3055\u308c\u305f\u30bb\u30f3\u30b5\u30fc\u304c\u9054\u6210\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b (Figure 5C<\/strong>). \u3053\u308c\u306f\u5148\u8ff0\u3057\u305f\u6709\u6a5f\u67d3\u6599\u3092\u7528\u3044\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u70b9\u3067\u4e00\u822c\u7684\u306b\u30b3\u30fc\u30c9\u5316\u3055\u308c\u305f\u529b\u5b66\u30bb\u30f3\u30b5\u30fc\u306b\u5bfe\u3057\u3066\u512a\u4f4d\u6027\u304c\u3042\u308a\uff0c\u86cd\u5149\u86cb\u767d\u8cea\u306e\u30bb\u30f3\u30b5\u30fc\u3088\u308a\u512a\u308c\u305f\u7279\u6027\u3092\u6301\u3064\uff0e\u4f7f\u7528\u3057\u305f\u86cd\u5149\u767a\u8272\u56e3\u306e\u30bb\u30c3\u30c8\u306f\u624b\u5143\u306e\u5b9f\u9a13\u3067\u3082\u9069\u5fdc\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\uff0c\u5358\u4e00\u7b4b\u7dda\u7dad\u4f38\u5c55\u5b9f\u9a13\u3084\u751f\u4f53\u5185\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306b\u3082\u9069\u5fdc\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b. HaloTag \u306b\u52a0\u3048\u3066\uff0c\u73fe\u5728\u306e\u30de\u30a6\u30b9\u30e2\u30c7\u30eb\u306f TEV \uff08\u30a6\u30a4\u30eb\u30b9\uff09\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u90e8\u4f4d\u3092\u3082\u6709\u3057\u3066\u3044\u308b\uff0e\u3053\u308c\u307e\u3067\u306e\u30bf\u30a4\u30c1\u30f3\u5206\u5b50\u3092\u5207\u65ad\u3057\u3088\u3046\u3068\u3059\u308b\u8a66\u307f (107<\/a>,124<\/a>,125<\/a>) \u3068\u9055\u3063\u3066\uff0c\u3053\u308c\u306f\u30bf\u30a4\u30c1\u30f3\u5206\u5b50\u306e\u534a\u5206\uff08\u30ce\u30c3\u30af\u30a4\u30f3\u306e\u30d8\u30c6\u30ed\u63a5\u5408\u4f53\u30de\u30a6\u30b9\uff09\u304b\uff0c\u5168\u30bf\u30a4\u30c1\u30f3\u5206\u5b50\uff08\u30db\u30e2\u63a5\u5408\u4f53\uff09\u3092\u9ad8\u5ea6\u306b\u7279\u7570\u7684\u306b\u5207\u65ad\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3042\u308a\uff0c\u305d\u308c\u306b\u3088\u308a\u7b4b\u5f3e\u6027\u306b\u304a\u3051\u308b\u30bf\u30a4\u30c1\u30f3\u306e\u5f79\u5272\u3068\u529b\u306e\u767a\u751f\u3092\u3088\u308a\u3088\u304f\u7406\u89e3\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\uff0e<\/p>\n

\u3000\u7b4b\u53ce\u7e2e\u306e\u8907\u96d1\u3055\u3092\u3088\u308a\u6df1\u304f\u63a2\u6c42\u3059\u308b\u3053\u3068\u306f\u53e4\u5178\u7684\u306a\u7b4b\u53ce\u7e2e\u5b9f\u9a13\u306e\u518d\u8a55\u4fa1\u3092\u8981\u3059\u308b\uff0e\u305d\u308c\u306b\u306f\u7b4b\u8089\u306e\u53d7\u52d5\u7684\u304a\u3088\u3073\u80fd\u52d5\u7684\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u4e21\u8005\u306b\u304a\u3044\u3066\u30bf\u30a4\u30c1\u30f3\u304c\u4e3b\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3059\u3053\u3068\u3092\u5ff5\u982d\u306b\u7f6e\u304f\u5fc5\u8981\u304c\u3042\u308b\uff0e\u30df\u30aa\u30b7\u30f3\u981a\u90e8\u306e\u8ffd\u5f93\u306b\u304a\u3051\u308b\u4e0b\u65b9\u50be\u5411\u3092\u9451\u307f\u308b\u306b (89<\/a>,126<\/a>,127<\/a>,128<\/a>), \u53ce\u7e2e\u3059\u308b\u30b5\u30eb\u30b3\u30e1\u30a2\u306e\u4e3b\u8981\u306a\u30a8\u30cd\u30eb\u30ae\u30fc\u84c4\u7a4d\u6e90\u306f\u518d\u8003\u3055\u308c\u306a\u304f\u3066\u306f\u306a\u3089\u306a\u3044\uff0e\u304a\u305d\u3089\u304f\u4e00\u822c\u306b\u30b3\u30fc\u30c9\u5316\u3055\u308c\u305f\u529b\u5b66\u30bb\u30f3\u30b5\u30fc\u306f, Huxley \u3068 Simmons \u306e\u53e4\u5178\u7684\u306a\u30af\u30a4\u30c3\u30af\u30ea\u30ea\u30fc\u30b9\u5b9f\u9a13 (9<\/a>) \u306e\u30d0\u30fc\u30b8\u30e7\u30f3\u3092\u518d\u8003\u3059\u308b\u3053\u3068\u3067\uff0c\u30bf\u30a4\u30c1\u30f3\u304c\u4e3b\u8981\u306a\u30a8\u30cd\u30eb\u30ae\u30fc\u84c4\u7a4d\u6e90\u3067\u3042\u308b\u3053\u3068\u3092\u8a3c\u660e\u3059\u308b\u3053\u3068\u306b\u306a\u308b\u3060\u308d\u3046\uff0e\u305d\u3057\u3066\u518d\u69cb\u7bc9\u3055\u308c\u305f\u30a2\u30af\u30c1\u30f3\u30df\u30aa\u30b7\u30f3\u8907\u5408\u4f53\u306e\u5358\u5206\u5b50\u5b9f\u9a13\u304c\u6539\u5584\u3057\u3064\u3064\u3042\u308a (26<\/a>,28<\/a>,29<\/a>), \u3055\u3089\u306b\u30df\u30aa\u30b7\u30f3\u306b\u3088\u308b\u529b\u306e\u767a\u751f\u30e1\u30ab\u30cb\u30ba\u30e0\u3078\u306e\u6d1e\u5bdf\u3092\u3082\u305f\u3089\u3057\u3064\u3064\u3042\u308b\u306b\u3082\u304b\u304b\u308f\u3089\u305a\uff0c\u7121\u50b7\u306e\u30b5\u30eb\u30b3\u30e1\u30a2\u5185\u90e8\u3067\u5358\u4e00\u306e\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306e\u901f\u5ea6\u3084\u30b9\u30c8\u30ed\u30fc\u30af\u30b5\u30a4\u30ba\u3092\u76f4\u63a5\u89b3\u5bdf\u3057\u305f\u5b9f\u9a13\u306f\u307e\u3060\u5b58\u5728\u3057\u306a\u3044\uff0e\u3053\u306e\u89b3\u70b9\u304b\u3089\u306f\uff0c\u305d\u306e\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u306e\u5206\u91ce\u306f\u30bf\u30a4\u30c1\u30f3\u3088\u308a\u5f8c\u306e\u6bb5\u968e\u3067\u3042\u308b\uff0e\u3055\u3089\u306b\uff0c\u71b1\u529b\u5b66\u30a8\u30f3\u30b8\u30f3\u306e\u3088\u3046\u306b\u7b4b\u8089\u3092\u6271\u3046\u3053\u3068\u306f, Fenn \u3084 Wilkie \u305d\u306e\u4ed6\u306e\u7814\u7a76\u8005\u3089\u304c\u71b1\u96fb\u5806\u3068\u71b1\u91cf\u8a08\u3092\u7528\u3044\u3066\u7b4b\u8089\u30a8\u30f3\u30bf\u30eb\u30d4\u30fc\u3092\u8a08\u6e2c\u3057\u305f\u7d50\u679c\uff0c\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u5358\u72ec\u306b\u71b1\u7523\u751f\u3092\u5272\u308a\u5f53\u3066\u3066\u3057\u307e\u3063\u305f\u3088\u3046\u306b\uff0c\u554f\u984c\u3092\u904e\u5ea6\u306b\u5358\u7d14\u5316\u3057\u3066\u3057\u307e\u3046 (27<\/a>,129<\/a>). \u5358\u7d14\u306a\u7c98\u5f3e\u6027\u30dd\u30ea\u30de\u30fc\u306f\u529b\u306e\u6e1b\u5c11\u306b\u4e57\u3058\u3066\u5de8\u5927\u306a\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u5897\u5927\u3092\u7d4c\u3066\uff0c\u5468\u8fba\u74b0\u5883\u304b\u3089\u71b1\u3092\u5438\u53ce\u3059\u308b\uff0e\u6298\u308a\u305f\u305f\u307e\u308c\u305f Ig \u30c9\u30e1\u30a4\u30f3\u306f\u5358\u7d14\u306a\u7c98\u5f3e\u6027\u30dd\u30ea\u30de\u30fc\u3067\u306f\u306a\u3044\uff0e\u30df\u30aa\u30b7\u30f3\u30e2\u30fc\u30bf\u30fc\u304c\u53c2\u52a0\u3057\u3066\u30bf\u30a4\u30c1\u30f3\u306b\u304b\u304b\u308b\u5916\u529b\u304c\u6e1b\u5c11\u3059\u308b\u6642\uff0c\u307e\u305a\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u5897\u52a0\u304c\u8d77\u304d\u3066\u304b\u3089\u30bf\u30a4\u30c1\u30f3\u304c\u71b1\u3092\u5438\u53ce\u3059\u308b\u306b\u9055\u3044\u306a\u3044\uff0e\u3057\u304b\u3057\uff0c\u30e2\u30eb\u30c6\u30f3\u30fb\u30b0\u30ed\u30d3\u30e5\u30fc\u30eb\u9077\u79fb\u306e\u5d29\u58ca\u306f\uff0c\u305d\u308c\u81ea\u4f53\u304c\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ce\u7e2e\u306e\u4e3b\u8981\u306a\u4e00\u90e8\u306a\u306e\u3060\u304c\uff0c\u9ad8\u5ea6\u306b\u30b3\u30f3\u30d1\u30af\u30c8\u306a\u5f62\u614b\u3092\u53d6\u308a\uff0c\u4f4e\u3044\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u3067\u306e\u9ad8\u6b21\u86cb\u767d\u8cea\u306b\u6298\u308a\u305f\u305f\u307e\u308c\u308b\u7d50\u679c\u3068\u306a\u308b\uff0e\u3086\u3048\u306b\uff0c\u86cb\u767d\u8cea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306f\u30a8\u30f3\u30c8\u30ed\u30d4\u30fc\u306e\u7d14\u640d\u5931\u3068\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\uff0c\u5468\u8fba\u306e\u30d0\u30b9\u3078\u306e\u71b1\u55aa\u5931\u306e\u7d50\u679c\u3068\u306a\u308b. Fenn \u3089\u306e\u7d50\u679c\u306f\u4eca\u3084\u518d\u8003\u3055\u308c\u308b\u5fc5\u8981\u304c\u3042\u308a\uff0c\u6211\u3005\u306f\u30bf\u30a4\u30c1\u30f3\u86cb\u767d\u8cea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u71b1\u529b\u5b66\u304c\u7c98\u5f3e\u6027\u30dd\u30ea\u30de\u30fc\u307b\u3069\u5358\u7d14\u3067\u306f\u306a\u3044\u3053\u3068\u3092\u77e5\u3063\u3066\u3044\u308b\uff0e<\/p>\n

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\u3000\u30bf\u30a4\u30c1\u30f3\u306e PEVK \u30c9\u30e1\u30a4\u30f3\u304c\u30a2\u30af\u30c1\u30f3\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u306b\u5dfb\u304d\u3064\u3044\u3066\u5f3e\u6027\u529b\u3092\u767a\u63ee\u3059\u308b\u3068\u3044\u3046 ‘winding filament’ \u4eee\u8aac\uff08\u30bf\u30a4\u30c1\u30f3\u306f’winding filament&#8 … <\/p>\n

“\u7b4b\u53ce\u7e2e\u306b\u304a\u3051\u308b\u4e3b\u8981\u306a\u99c6\u52d5\u529b\u3068\u3057\u3066\u306e\u30bf\u30a4\u30c1\u30f3\u86cb\u767d\u306e\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u50cd\u304d” \u306e<\/span>\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[2330,2073,2303,2213,2208,2244,2352,2214,2309,2168,2215,2267,2221,2343,2335,2232,2226,2292,2314,2072,2266,2258,2211,2231,102,2313,2264,2066,2209,2216,2227,2217,2212,2236,2068,2237,2274,2219,2218,2276,2305,2220,2351,2203,2355,2210,2170,2353,2169,2295,2200,100,2329,2344,70,2223,2037,2299,2323,2229,2206,2027,2256,2268,2224,2269,2300,2347,2246,2294,2356,2349,2239,2310,97,2288,2286,2289,2162,2339,101,2243,2341,2316,2296,2202,2332,2240,2291,2308,2233,2253,2311,2340,2116,2198,2301,2287,2284,2324,2238,71,2222,2328,2279,2270,2278,2262,2241,2321,2250,2076,2312,2275,2205,2325,2249,2062,1638,2346,2293,2298,2336,2263,2167,2364,2077,2074,2297,2245,2207,2199,2036,2204,2283,2282,2085,2304,2350,2307,2342,2281,2319,2225,2306,2318,2277,2260,2280,2235,2357,2359,2358,2354,2327,2302,2271,2261,2345,2248,2201,2247,1689,2360,2361,2363,2075,2317,2333,2265,2331,2285,2290,2272,2348,2337,2251,2334,2234,2228,2257,2242,2273,2322,2320,2254,2255,2326,2259,2338,2315,2230,2362,2252],"class_list":{"0":"post-3488","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"hentry","6":"category-resistance-training","7":"tag-2330","8":"tag-a-band","9":"tag-active-force","10":"tag-actomyosin-crossbridges","11":"tag-atomic-force-microscopy","12":"tag-atp","13":"tag-clip-tag","14":"tag-entropic-recoi","15":"tag-f-","16":"tag-folding","17":"tag-folding-contraction","18":"tag-force-clamp","19":"tag-force-spectroscopy","20":"tag-fret","22":"tag-halotag","23":"tag-henry-eryin","24":"tag-hill","25":"tag-hookean","26":"tag-i-band","27":"tag-i10","28":"tag-i91","29":"tag-ig-domains","30":"tag-iii","31":"tag-ii","32":"tag-i","33":"tag-kuhn","34":"tag-m-line","35":"tag-magnetic-tweezers","36":"tag-mechanical-battery","37":"tag-milton-polissar","38":"tag-muscle-contraction","39":"tag-myosin-motor","40":"tag-n2b","41":"tag-pevk","43":"tag-phi-value-analysis","44":"tag-polymer-physics","45":"tag-protein-folding","46":"tag-residual-force","47":"tag-s-","48":"tag-single-molecule","49":"tag-snap-tag","50":"tag-technology-will-always-win","51":"tag-tev","52":"tag-titin","53":"tag-titin-folding","54":"tag-tmp-tag","55":"tag-unfolding","56":"tag-v","57":"tag-winding-filament","58":"tag-z","59":"tag-b-","60":"tag-2344","61":"tag-70","62":"tag-2223","63":"tag-2037","64":"tag-2299","65":"tag-2323","66":"tag-2229","67":"tag-2206","68":"tag-2027","69":"tag-2256","70":"tag-2268","71":"tag-2224","72":"tag-2269","73":"tag-2300","74":"tag-2347","75":"tag-2246","76":"tag-2294","77":"tag-2356","78":"tag-2349","79":"tag-2239","80":"tag-2310","81":"tag-97","82":"tag-2288","83":"tag-2286","84":"tag-2289","85":"tag-2162","86":"tag-2339","87":"tag-101","88":"tag-2243","89":"tag-2341","90":"tag-2316","91":"tag-2296","92":"tag-2202","93":"tag-2332","94":"tag-2240","95":"tag-2291","96":"tag-2308","97":"tag-2233","98":"tag-2253","99":"tag-2311","100":"tag-2340","101":"tag-2116","102":"tag-2198","103":"tag-2301","104":"tag-2287","105":"tag-2284","106":"tag-2324","107":"tag-2238","108":"tag-71","109":"tag-2222","110":"tag-2328","111":"tag-2279","112":"tag-2270","113":"tag-2278","114":"tag-2262","115":"tag-2241","116":"tag-2321","117":"tag-2250","118":"tag-2076","119":"tag-2312","120":"tag-2275","121":"tag-2205","122":"tag-2325","123":"tag-2249","124":"tag-2062","125":"tag-1638","126":"tag-2346","127":"tag-2293","128":"tag-2298","129":"tag-2336","130":"tag-2263","131":"tag-2167","132":"tag-2364","133":"tag-2077","134":"tag-2074","135":"tag-2297","136":"tag-2245","137":"tag-2207","138":"tag-2199","139":"tag-2036","140":"tag-2204","141":"tag-2283","142":"tag-2282","143":"tag-2085","144":"tag-2304","145":"tag-2350","146":"tag-2307","147":"tag-2342","148":"tag-2281","149":"tag-2319","150":"tag-2225","151":"tag-2306","152":"tag-2318","153":"tag-2277","154":"tag-2260","155":"tag-2280","156":"tag-2235","157":"tag-2357","158":"tag-2359","159":"tag-2358","160":"tag-2354","161":"tag-2327","162":"tag-2302","163":"tag-2271","164":"tag-2261","165":"tag-2345","166":"tag-2248","167":"tag-2201","168":"tag-2247","169":"tag-1689","170":"tag-2360","171":"tag-2361","172":"tag-2363","173":"tag-2075","174":"tag-2317","175":"tag-2333","176":"tag-2265","177":"tag-2331","178":"tag-2285","179":"tag-2290","180":"tag-2272","181":"tag-2348","182":"tag-2337","183":"tag-2251","184":"tag-2334","185":"tag-2234","186":"tag-2228","187":"tag-2257","188":"tag-2242","189":"tag-2273","190":"tag-2322","191":"tag-2320","192":"tag-2254","193":"tag-2255","194":"tag-2326","195":"tag-2259","196":"tag-2338","197":"tag-2315","198":"tag-2230","199":"tag-2362","200":"tag-2252"},"_links":{"self":[{"href":"https:\/\/www.muscle-hypertrophy.com\/index.php?rest_route=\/wp\/v2\/posts\/3488","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.muscle-hypertrophy.com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